A5047472902- Neurogenesis and neuroplasticity mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Glioma Diagnosis and Treatment
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Mitochondrial Function and Pathology
- PARP inhibition in cancer therapy
- Urological Disorders and Treatments
- CRISPR and Genetic Engineering
- Renal and related cancers
- Cancer-related gene regulation
- Genetics, Aging, and Longevity in Model Organisms
- Anesthesia and Neurotoxicity Research
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Genomic variations and chromosomal abnormalities
- TGF-β signaling in diseases
- ATP Synthase and ATPases Research
- Carcinogens and Genotoxicity Assessment
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2007-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2002-2024
Inserm
2005-2024
Université Paris Cité
2007-2024
Université Paris-Saclay
2023-2024
CEA Paris-Saclay
2023-2024
Paracelsus Medical University
2023-2024
Austrian Cluster for Tissue Regeneration
2023-2024
Institut de Radiobiologie Cellulaire et Moléculaire
2011-2023
Institut de Biologie Paris-Seine
2023
Abstract Background Spermatogenesis in adult is a complex stepwise process leading to terminally differentiated spermatozoa. The cellular heterogeneity of testis renders the studies on molecular aspects this differentiation process. Analysis regulation spermatogenesis would undoubtedly benefit from development techniques characterize each germinal step. Methods Hoechst 33342 staining mouse testicular cells allows characterization an enriched population stem cell and spermatogonia, called...
Research Article25 March 2013Open Access Source Data Vascular-derived TGF-β increases in the stem cell niche and perturbs neurogenesis during aging following irradiation adult mouse brain Jose R. Pineda CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, Fontenay-aux-Roses, France INSERM, U967, Université Paris Diderot, Sorbonne Cité, UMR 967, Sud, Search for more papers by this author Mathieu Daynac Alexandra Chicheportiche Arantxa Cebrian-Silla Laboratorio Neurobiología Comparada, Instituto...
Besides the established central role of poly(ADP-ribose) polymerase-1 (Parp-1) and Parp-2 in maintenance genomic integrity, accumulating evidence indicates that poly(ADP-ribosyl)ation may modulate epigenetic modifications under physiological conditions. Here, we provide vivo for pleiotropic involvement both meiotic postmeiotic processes. We show Parp-2-deficient mice exhibit severely impaired spermatogenesis, with a defect prophase meiosis I characterized by massive apoptosis at pachytene...
Abstract Although neural stem cells (NSCs) sustain continuous neurogenesis throughout the adult lifespan of mammals, they progressively exhibit proliferation defects that contribute to a sharp reduction in subventricular during aging. However, little is known regarding early age-related events neurogenic niches. Using fluorescence-activated cell sorting technique allows for prospective purification main populations from zone (SVZ), we demonstrated an decline with dramatic loss progenitor 4...
Highlights•Transcriptome analysis reveals molecular hallmarks of activated and quiescent NSCs•Data resource putative markers and/or regulators NSC quiescence•Quiescent NSCs integrate various signals from the microenvironment•Syndecan-1 is involved in proliferation NSCsSummaryDeciphering mechanisms that regulate quiescence adult neural stem cells (NSCs) crucial for development therapeutic strategies based on stimulation their endogenous regenerative potential damaged brain. We show LeXbright...
Meiotic DNA double strand breaks (DSBs) are indicated at leptotene by the phosphorylated form of histone H2AX (γ-H2AX). In contrast to previous studies, we identified on both zygotene and pachytene chromosomes two distinct types γ-H2AX foci: multiple small (S) foci located along autosomal synaptonemal complexes (SCs) larger signals chromatin loops (L-foci). The S-foci number gradually declined throughout pachytene, in parallel with repair DSBs monitored proteins suggesting that mark DSB...
The cyclin‐dependent kinase inhibitor p21waf1/cip mediates the p53‐dependent G1/S checkpoint, which is generally considered to be a critical requirement maintain genomic stability after DNA damage. We used staggered 5‐ethynyl‐2′deoxyuridine/5‐bromo‐2′‐deoxyuridine double‐labeling in vivo investigate cell cycle progression and role of damage response neural stem progenitor cells (NSPCs) exposure developing mouse cortex ionizing radiation. observed radiation‐induced p21‐dependent apoptotic...
Neurogenesis decreases during aging causing a progressive cognitive decline but it is still controversial whether proliferation defects in neurogenic niches result from loss of neural stem cells or an impairment their progression through the cell cycle. Using accurate fluorescence-activated sorting technique, we show that pool maintained subventricular zone middle-aged mice while they have reduced proliferative potential eventually leading to subsequent decrease progeny. In addition,...
Neonatal gonocytes are the precursors of both spermatogonial stem cells and spermatogonia; thus, any persistant DNA damage in these may lead to heritable mutations. We investigated response male mouse neonatal germ ionizing radiation. Both spermatogonia died large numbers by apoptosis. However, we found that were significantly more sensitive than somatic radiation-induced double-strand breaks (DSBs), as assayed number gamma-H2AFX foci. In contrast, irradiated G2 phase seemed repair DSBs...
The lateral wall of the subventricular zone harbors neural stem cells (NSC, B cells) which generate proliferating transient-amplifying progenitors (TAP, C that ultimately give rise to neuroblasts (NB, A cells). Molecular profiling at single cell level struggles distinguish these different types. Here, we combined transcriptome analyses FACS-sorted and single-cell RNAseq demonstrate existence an abundant, clonogenic multipotent population immature (iNB transition between TAP migrating NB...
The lateral wall of the mouse subventricular zone harbors neural stem cells (NSC, B cells) which generate proliferating transient-amplifying progenitors (TAP, C that ultimately give rise to neuroblasts (NB, A cells). Molecular profiling at single-cell level struggles distinguish these different cell types. Here, we combined transcriptome analyses FACS-sorted and RNAseq demonstrate existence an abundant, clonogenic multipotent population immature (iNB transition between TAP migrating NB...
Summary The lateral wall of the subventricular zone harbors neural stem cells (NSC, B cells) which generate proliferating transient-amplifying progenitors (TAP, C that ultimately give rise to neuroblasts (NB, A cells). Molecular profiling at single cell level struggles distinguish these different types. Here, we combined transcriptome analyses FACS-sorted and single-cell RNAseq demonstrate existence an abundant, clonogenic multipotent population immature (iNB transition between TAP migrating...
Summary The lateral wall of the subventricular zone harbors neural stem cells (NSC, B cells) which generate proliferating transient-amplifying progenitors (TAP, C that ultimately give rise to neuroblasts (NB, A cells). Molecular profiling at single cell level struggles distinguish these different types. Here, we combined transcriptome analyses FACS-sorted and single-cell RNAseq demonstrate existence an abundant, clonogenic multipotent population immature (iNB or D transition between TAP...
The lateral wall of the mouse subventricular zone harbors neural stem cells (NSC, B cells) which generate proliferating transient-amplifying progenitors (TAP, C that ultimately give rise to neuroblasts (NB, A cells). Molecular profiling at single-cell level struggles distinguish these different cell types. Here, we combined transcriptome analyses FACS-sorted and RNAseq demonstrate existence an abundant, clonogenic multipotent population immature (iNB transition between TAP migrating NB...
The evolutionary conservation of the human chitotriosidase gene