A5074556185- Hormonal Regulation and Hypertension
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal Imaging and Analysis
- Ion Transport and Channel Regulation
- Catalytic Processes in Materials Science
- Pancreatic function and diabetes
- Advanced Chemical Physics Studies
- Sodium Intake and Health
- Epigenetics and DNA Methylation
- Cardiac Valve Diseases and Treatments
- Catalysis and Oxidation Reactions
- Retinal and Optic Conditions
- Advanced Fiber Optic Sensors
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Metal Forming Simulation Techniques
- Chronic Kidney Disease and Diabetes
- Electrolyte and hormonal disorders
- Electronic and Structural Properties of Oxides
- Birth, Development, and Health
- Optical Coherence Tomography Applications
- Blood Pressure and Hypertension Studies
- Mechanical Circulatory Support Devices
- Endoplasmic Reticulum Stress and Disease
International University of Health and Welfare
2017-2025
University of Fukui Hospital
2024-2025
Osaka University
2003-2024
The University of Tokyo
2015-2024
University of Tokyo Hospital
2014-2024
Nagoya University Hospital
2020-2024
Yokohama City University
2021-2023
Nippon Steel (Japan)
2001-2023
Inserm
2023
Sorbonne Université
2023
Epigenetic mechanisms may underlie the progression of diabetic kidney disease. Because is a heterogeneous organ with different cell types, we investigated DNA methylation status in type-specific manner. We first identified genes specifically demethylated normal proximal tubules obtained from control db/m mice, and next delineated candidate disease-modifying bearing aberrant induced by diabetes using db/db mice. Genes involved glucose metabolism, including Sglt2, Pck1, G6pc, were selectively...
Experiments with hyperaldosteronemic animals suggest that, despite lowering plasma aldosterone, salt worsens renal injury by paradoxical activation of the mineralocorticoid receptor (MR). Salt and aldosterone synergistically contribute to impairment through Rac1-mediated MR, but whether angiotensin II also promotes this mechanism is unknown. Here, we placed II-overproducing double transgenic Tsukuba hypertensive mice on a low- or high-salt intake for 6 weeks treated some adrenalectomy, MR...
Overactivation of the mineralocorticoid receptor signaling is implicated in cardiovascular disease, including hypertensive heart disease. Oxidative stress suggested to augment signal transduction, but precise mechanisms remain unclear. Mineralocorticoid activity regulated by multiple factors, addition plasma ligand levels. We previously identified Rac1 GTPase as a modulator activity. Here we show that oxidative induces activation ligand-independent, Rac1-depenent manner cardiomyocytes....
There is increasing evidence for a crucial role of aberrant mineralocorticoid receptor (MR) activation in heart failure, with clinical studies showing beneficial effects MR blockade. However, the mechanisms failure remain unclear. In this study, we observed that small GTPase Rac1 contributes to myocardial activation, whereas Rac1-MR pathway leads cardiac dysfunction. Mouse hearts subjected chronic pressure overload induced by transverse aortic constriction showed and increased nuclear...
Aging is associated with a high prevalence of hypertension due to elevated susceptibility BP dietary salt, but its mechanism unknown. Serum levels Klotho, an anti-aging factor, decline age. We found that salt (HS) increased in aged mice and young heterozygous Klotho-knockout was vascular expression Wnt5a p-MYPT1, which indicate RhoA activity. Not only the Wnt inhibitor LGK974 antagonist Box5 Klotho supplementation inhibits HS-induced elevation, similarly Rho kinase fasudil, reduced p-MYPT1...
Utilization of carbon dioxide (CO
Objective : The WFS1 gene encodes an endoplasmic reticulum (ER) membrane-embedded protein. Homozygous mutations cause Wolfram syndrome, characterized by insulin-deficient diabetes mellitus and optic atropy. Pancreatic β-cells are selectively lost from the patient’s islets. ER localization suggests that protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of calcium homeostasis. Disturbances or overloading these induces stress responses,...
Abstract A cytocompatible inkjet bioprinting approach that enables the use of a variety bioinks to produce hydrogels with wide range characteristics is developed. Stabilization caused by horseradish peroxidase (HRP)‐catalyzed cross‐linking consuming hydrogen peroxide (H 2 O ). 3D cell‐laden are fabricated sequential dropping bioink containing polymer(s) cross‐linkable through enzymatic reaction and H onto droplets another polymer, HRP, cells. The ≈95% viability enclosed mouse fibroblasts...
<b><i>Background/Aims:</i></b> Obesity and diabetes are intimately interrelated, independent risk factors for kidney disease. Overactivation of mineralocorticoid receptor (MR) is implicated in end organ damage both pathologies. But the underlying mechanism MR activation remains uncertain. We explored involvement Rac1, which we previously identified as a ligand-independent activator, renal vitro vivo. <b><i>Methods:</i></b> evaluated activity...
Maternal malnutrition, which causes prenatal exposure to excessive glucocorticoid, induces adverse metabolic programming, leading hypertension in offspring. In offspring of pregnant rats receiving a low-protein diet or dexamethasone, synthetic mRNA expression angiotensin receptor type 1a (Agtr1a) the paraventricular nucleus (PVN) hypothalamus was upregulated, concurrent with reduced DNA methyltransferase 3a (Dnmt3a), binding DNMT3a Agtr1a gene, and demethylation. Salt loading increased BP...
Pulmonary fibrosis is the result of abnormal processes repair that occur after lung injury. Transforming growth factor (TGF)-beta a key molecule in progression pulmonary fibrosis. Although clinical use interferon (IFN)-beta did not improve survival patients with idiopathic fibrosis, because some preclinical studies have suggested IFN-beta potent inhibitor fibrogenesis, beneficial effects been expected. We therefore attempted to determine and investigated mechanism action bleomycin-induced...
To elucidate the genetic etiology of persistent hyperinsulinemic hypoglycemia infancy (PHHI) in Japanese population, we conducted a polymerase chain reaction-single-strand conformation polymorphism analysis sulfonylurea receptor 1 (SUR1) and Kir6.2 genes 17 PHHI patients, including pair siblings from consanguineous family. We also analyzed glutamate dehydrogenase gene for exons encoding an allosteric regulatory domain enzyme. In SUR1 gene, identified one frameshift (I446fsdelT) two missense...
Genome-wide analysis of renal sodium-transporting system has identified specific variations Mendelian hypertensive disorders, including HSD11B2 gene variants in apparent mineralocorticoid excess. However, these genetic extrarenal tissue can be involved developing hypertension, as demonstrated former studies using global and brain-specific Hsd11b2 knockout rodents. To re-examine the importance dysfunction on we generated kidney-specific mice. The mice exhibited systemic which was abolished by...
The renin-angiotensin-aldosterone system has an important role in the control of fluid homeostasis and BP during volume depletion. Dietary salt restriction elevates circulating angiotensin II (AngII) aldosterone levels, increasing levels Cl − /HCO 3 exchanger pendrin β -intercalated cells Na + -Cl cotransporter (NCC) distal convoluted tubules. However, independent roles AngII regulating these remain unclear. In C57BL/6J mice receiving a low-salt diet or infusion, we evaluated membrane...
We have previously shown that podocyte injury increases the glomerular filtration of liver-derived Agt (angiotensinogen) and generation intrarenal Ang II (angiotensin II) filtered is reabsorbed by proximal tubules in a manner dependent on megalin. In present study, we aimed to study role megalin renal sodium handling during nephrotic syndrome. generated tubule-specific KO (knockout) mice crossed these animals with NEP25 mice, which podocyte-specific can be induced injection immunotoxin LMB2....
NO reduction by CO on Rh(111) was investigated near-ambient pressure X-ray photoelectron spectroscopy, mass spectrometry, and kinetic analysis. Under exposure to + mixed gases with heating the surface from room temperature 450 °C, dissociation reaction start simultaneously independent of gas ratio NO/CO, which indicates that triggers this reaction. From analyses based observed adsorbate coverages under conditions, following two points are suggested: (i) NOhollow is a reactive species for N2...
The expression of NGAL/lcn2 (neutrophil gelatinase-associated lipocalin) is directly modulated by mineralocorticoid receptor activation but its role in blood pressure control unclear.a potential relationship between NGAL plasma levels, systolic and urinary Na excretion was assessed the STANISLAS cohort. specific salt-sensitive hypertension studied using lcn2-knockout mice (lcn2 KO) fed with low-Na diet (0Na).we show that levels positively correlate pressure, whereas they negatively subjects...
Introduction: Prognostic factor investigations for candidemia have been conducted in large-scale facilities, leading to significant evidence, including early administration of echinocandin antifungal agents and removal central venous catheters (CVCs). In departments that provide aggressive chemotherapy or transplantation, candidiasis markers are regularly evaluated, preemptive treatments may be initiated. However, resource-limited detection largely relies on vital signs like fever blood...
Significance Statement Pendrin expressed in renal β -intercalated cells is involved sodium chloride reabsorption distal nephron, and plays an essential role fluid homeostasis BP control conjunction with cotransporter convoluted tubules. Using intercalated cell–specific mineralocorticoid receptor knockout mice, the authors found two distinct pathways of pendrin activation: by angiotensin II elevation, mediated cells, hypokalemic alkalosis, principal cells. Moreover, they demonstrated that...