A5103016997- Cell Adhesion Molecules Research
- RNA Research and Splicing
- RNA modifications and cancer
- Advanced Proteomics Techniques and Applications
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- vaccines and immunoinformatics approaches
- Endoplasmic Reticulum Stress and Disease
- Bacteriophages and microbial interactions
- Chronic Myeloid Leukemia Treatments
- Chronic Lymphocytic Leukemia Research
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- PI3K/AKT/mTOR signaling in cancer
- Cervical and Thoracic Myelopathy
- Hemoglobinopathies and Related Disorders
- Cell Image Analysis Techniques
- Histone Deacetylase Inhibitors Research
- Science, Research, and Medicine
- Dental Education, Practice, Research
- Autopsy Techniques and Outcomes
- Autophagy in Disease and Therapy
- Voice and Speech Disorders
Norwegian University of Life Sciences
2024
Oslo University Hospital
2017-2023
University of Oslo
2017-2023
Norwegian Cancer Society
2018-2020
Wellcome Centre for Cell-Matrix Research
2015-2017
University of Manchester
2015-2017
University of Georgia
2008
University of Cincinnati
1980
Cell-extracellular matrix (ECM) adhesion is a fundamental requirement for multicellular existence due to roles in positioning, proliferation and differentiation. Phosphorylation plays major role signalling; however, full understanding of the phosphorylation events that occur at sites lacking. Here we report proteomic phosphoproteomic analysis complexes isolated from cells spread on fibronectin. We identify 1,174 proteins, 499 which are phosphorylated (1,109 sites), including both...
Genomics is increasingly applied in breeding programmes for farmed fish and shellfish species around the world. However, current applications do not include information on genome functional activity, which can enhance opportunities to predict relationships between genotypes phenotypes hence increase accuracy of selection. Here, we review prospects improving aquaculture practises through uptake genomics data light EU Horizon 2020 project AQUA-FAANG: ‘Advancing European Aquaculture by Genome...
Abstract The integration of cells with their extracellular environment is facilitated by cell surface adhesion receptors, such as integrins, which play important roles in both normal development and the onset pathologies. Engagement integrins ligands matrix, or counter‐receptors on other cells, initiates intracellular assembly a wide variety proteins into complexes focal contacts, adhesions, fibrillar adhesions. recruited to these mediate bidirectional signaling across plasma membrane, and,...
Significance How organisms maintain homeostasis when confronted with environmental stress is an important question in biology. The master nutrient response regulator target of rapamycin complex 1 (TORC1) regulates many progrowth cellular processes, including transcription genes required for protein synthesis. RNA polymerase III (RNAPIII) plays a crucial role regulation synthesis by transcribing tRNA genes. RNAPIII activity known to be dependent on TORC1, but the underlying molecular...
tRNA genes are transcribed by RNA polymerase III (RNAPIII). During recent years it has become clear that RNAPIII activity is strictly regulated the cell in response to environmental cues and homeostatic status of cell. However, molecular mechanisms control regulate amplitude tDNA transcription normally cycling cells not well understood. Here, we show levels fluctuate during cycle reveal an underlying mechanism. The cyclin Clb5 recruits dependent kinase Cdk1 boost late S phase. At genes,...
Abstract Motivation: Searching genomes for non-coding RNAs (ncRNAs) by their secondary structure has become an important goal bioinformatics. For pseudoknot-free structures, ncRNA search can be effective based on the covariance model and CYK-type dynamic programming. However, computational difficulty in aligning RNA sequence to a pseudoknot prohibited fast accurate of arbitrary structures. Our previous work introduced graph pseudoknots proposed solve structure–sequence alignment...
Post-translational modification by small ubiquitin-like modifier (Sumo) regulates many cellular processes, including the adaptive response to various types of stress, referred as Sumo stress (SSR). However, it remains unclear whether SSR involves a common set core proteins regardless type or each particular induces stress-specific that targets unique, largely nonoverlapping substrates. In this study, we used MS and Gene Ontology approach identify differentially sumoylated during heat...
Internal tandem duplications in the tyrosine kinase receptor FLT3 (FLT3-ITD) are among most common lesions acute myeloid leukemia and there exists a need for new forms of treatment.Using ex vivo drug sensitivity screening, we found that FLT3-ITD+ patient cells particularly sensitive to HSP90 inhibitors.While it is well known important FLT3-ITD stability, family members play much more complex role signaling than previously appreciated.First, activates unfolded protein response, leading...
Breakpoint Cluster Region-Abelson kinase (BCR-Abl) is a driver oncogene that causes chronic myeloid leukemia and subset of acute lymphoid leukemias. Although tyrosine inhibitors provide an effective treatment for these diseases, they generally do not kill leukemic stem cells (LSCs), the cancer-initiating compete with normal hematopoietic bone marrow niche. New strategies to target cancers driven by BCR-Abl are therefore urgently needed. We performed small molecule screen based on competition...
The antibiotic-tolerant biofilms present in tuberculous granulomas add an additional layer of complexity when treating mycobacterial infections, including tuberculosis (TB). For a more efficient treatment TB, the biofilm forms mycobacteria warrant specific attention. Here, we used Mycobacterium marinum (Mmr) as biofilm-forming model to identify abundant proteins covering surface. We biotinylation/streptavidin-based proteomics on exposed at Mmr matrices vitro 448 and ex vivo detect 91 from...
Abstract BCR-Abl is a driver oncogene that causes chronic myeloid leukemia and subset of acute lymphoid leukemias. Although tyrosine kinase inhibitors provide an effective treatment for these diseases, they generally do not kill leukemic stem cells. Leukemic cells are cancer-initiating compete with normal hematopoietic the bone marrow niche. Using as model oncogene, we performed drug screen based on competition between isogenic untransformed BCR-Abl-transformed cells, identified several...
Abstract Tissue-penetrating nanobodies as drug-delivery vehicles have shown efficacy in treating hard-to-reach and -treat diseases. The present study reports with application potential the treatment of tuberculosis (TB), especially antibiotic tolerant biofilms that form TB granulomas. Using Mycobacterium marinum (Mmr) a model pathogen, we identified most abundant matrix proteins cultured granuloma from infected zebrafish, used them targets to produce synthetic (sybodies). Surface-exposed on...
Human polymorphonuclear leukocytes (PMN) die, apparently nonspecifically, in the standard tissue typing assay. When cell preparation contains more than 20% PMN, proper evaluation of histocompatibility assay is difficult. PMN were noted to aggregate when normal rabbit serum was added as complement source. Based on latter observation, a simple technique developed which reduces contamination suspension test cells and returns high yields lymphocytes. Removal lowers background dead permits...