A5102801597- Zebrafish Biomedical Research Applications
- Anesthesia and Sedative Agents
- Anesthesia and Neurotoxicity Research
- Advanced Neuroimaging Techniques and Applications
- Hematopoietic Stem Cell Transplantation
- Intensive Care Unit Cognitive Disorders
- Dementia and Cognitive Impairment Research
- Cardiac, Anesthesia and Surgical Outcomes
- Biofuel production and bioconversion
- Immune cells in cancer
- Folate and B Vitamins Research
- Anesthesia and Pain Management
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Ophthalmology and Visual Impairment Studies
- Telomeres, Telomerase, and Senescence
- MRI in cancer diagnosis
- Barrier Structure and Function Studies
- Microbial Inactivation Methods
- Advanced Glycation End Products research
- Eosinophilic Esophagitis
- Angiogenesis and VEGF in Cancer
- Ultrasound and Hyperthermia Applications
- Nausea and vomiting management
- Heme Oxygenase-1 and Carbon Monoxide
- Trigeminal Neuralgia and Treatments
Harvard Stem Cell Institute
2021-2024
Harvard University
2021-2024
Howard Hughes Medical Institute
2021-2024
Boston Children's Hospital
2021-2024
Dana-Farber Cancer Institute
2021-2023
Kosin University Gospel Hospital
2021-2022
Kosin University
2013-2021
Pain and Rehabilitation Medicine
2020
Chungnam National University
2019
Seoul National University
2017
Tissue-specific stem cells persist for a lifetime and can differentiate to maintain homeostasis or transform initiate cancer. Despite their importance, there are no described quality assurance mechanisms newly formed cells. We observed intimate specific interactions between macrophages nascent blood in zebrafish embryos. Macrophage frequently led either removal of cytoplasmic material cell division complete engulfment death. Stressed were marked by surface Calreticulin, which stimulated...
Macrophages maintain hematopoietic stem cell (HSC) quality by assessing surface Calreticulin (Calr), an “eat-me” signal induced reactive oxygen species (ROS). Using zebrafish genetics, we identified Beta-2-microglobulin (B2m) as a crucial “don’t eat-me” on blood cells. A chemical screen revealed inducers of Calr that promoted HSC proliferation without triggering ROS or macrophage clearance. Whole-genome CRISPR-Cas9 screening showed Toll-like receptor 3 (Tlr3) signaling regulated b2m...
The hematopoietic niche is a supportive microenvironment composed of distinct cell types, including specialized vascular endothelial cells that directly interact with stem and progenitor (HSPCs). molecular factors specify orchestrate HSPC homeostasis remain largely unknown. Using multi-dimensional gene expression chromatin accessibility analyses in zebrafish, we define conserved signature cis-regulatory landscape are unique to sinusoidal the niche. enhancer mutagenesis transcription factor...
Sang-Su Kim, Cheol Hong Ji Wook Hsi Chiang Kung, Tae Woo Park, Yu Som Shin, Ju Deok Siejeong Ryu, Wang-Joon Yung Hyun Choi, and Kyoung Seob Song. BMB Reports 2017;50:516-21. https://doi.org/10.5483/BMBRep.2017.50.10.100
Background Inhalation anesthetics are an important factor for postoperative hepatic and renal dysfunction. In this regard, TIVA can reduce the risk of dysfunction inherited to inhalation anesthetics. The present study was conducted determine whether functions differ after anesthesia with sevoflurane propofol. Methods Two hundred patients, ASA physical status class I, II, scheduled elective thyroidectomy were randomly divided into two groups. Anesthesia maintained 1-2% remifentanil in group...
Abstract Although diesel airborne particulate matter (PM2.5) has been known to play a role in many human diseases, there is no direct evidence that therapeutic drugs or proteins can diminish PM2.5‐induced diseases. Nevertheless, studies examining the negative control mechanisms of diseases are critical develop novel medications. In this study, consensus PDZ peptide ZO‐1 inhibited inflammatory cell infiltration, pro‐inflammatory cytokine gene expression, and TEER bronchoalveolar lavage (BAL)...
초음파는 세포사멸을 포함하여 의학 및 생물학분야에 널리 응용되고 있으나 그 정확한 기작에 대해선 논쟁의 여지가 있다. 본 연구에서는 40 kH 초음파 조사시스템을 단세포 효모에 적합하게 개발하고 세포사멸 유도시 초음파의 생물학적 현상을 살펴보았다. 아이오딘화 칼륨 선량 측정법을 이용하여 1.5 ml 실험튜브에 조사 시스템의 최적 조건을 맞추어 시간 의존적 방식으로 연구하였고 조사과정동안 온열효과와는 별개로 세포 사멸이 관찰되었다. 초음파와 과산화수소의 동시 처리는 세포사멸에 상조적인 효과가 관찰되어 활성산소가 초음파사멸에 관련이 있었다. 그러나 활성산소 저해제, NAC(N-acetyl-Lcysteine)는 초음파에 의한 약한 영향만을 미쳤고 다른 세포사멸, 괴사억제제[글리실리진(glycyrrhizin) 또는 zVAD-fmk] 역시도 완전히 억제하진 못하였다. 연구를 통하여 세포사멸에는 온열효과나 활성산소만으로 유도되지는 않는 것으로 보인다. Ultrasound has been...
While sevoflurane and desflurane have been regarded as inhalation agents providing rapid induction emergence, previous studies demonstrated the superiority of desflurane-anesthesia compared to sevoflurane-anesthesia in postoperative recovery obese geriatric patients. We investigated whether a short-term switch at end enhances patient profile non-obese randomly divide patients undergoing elective surgery (n = 60) into two groups: group (Group-S, <math...
Abstract The hematopoietic niche is a supportive microenvironment comprised of distinct cell types, including specialized vascular endothelial cells that directly interact with stem and progenitor (HSPCs). molecular factors specify orchestrate HSPC homeostasis remain largely unknown. Using multi-dimensional gene expression chromatin accessibility analyses, we define conserved signature cis -regulatory landscape unique to sinusoidal in the niche. enhancer mutagenesis transcription factor...
<h2>Abstract</h2> Hematopoietic stem cell transplantation requires a collection of hematopoietic cells from patients or donors. Granulocyte colony-stimulating factor (G-CSF) is widely used in the clinic to mobilize and progenitor (HSPCs) adult bone marrow niche into circulation, allowing HSPCs blood. The mechanism by which G-CSF acts unclear, with some studies showing direct stimulation others suggesting that myeloid are required. In this study, we developed heat-inducible transgenic...
서 론 부위마취 시행 시의 단점은 환자의 수술 중 불안 감과 각성이 환자에게 불편감과 스트레스를 줄 수 있다는 것인데, midazolam이나 propofol 같 은 진정제를 단독 투여하거나 alfentanil이나 remi-fentanil과 같은 아편양제제를 병용 투여함으로써 환 자의 긴장완화와 협조적 상태를 유도할 있다. 1) 결장내시경(colonoscopy) 환자에서 실시한 propofol과 alfentanil을 이용한 진정 자가조절법(patient-controlled sedation)은 diazepam과 meperidine 정주법에 비하여
Apolipoprotein E (APOE) ϵ4 allele is a very well-known genetic risk factor of Alzheimer's disease (AD). Recent histopatholgical and MRI studies in patients with AD demonstrated alterations white matter, as well gray matter. This study aimed to investigate possible associations between cerebral matter changes APOE genotype early stage AD. The authors used Diffusion Tensor Imaging (DTI) explore detect pathological processes. 26 participants were included the which 13 carriers age-, gender-,...
This study aimed to investigate the atrophy of posterior cingulate cortex (PCC) and medical temporal lobe (MTL) structures (i.e., entorhinal (ERC) hippocampus) regional disruption cingulum bundle in mild cognitive impairment (MCI) Alzheimer's disease (AD) patients. The relationships between these structure were also explored. Three-dimensional MRI diffusion tensor imaging applied 19 MCI, probable AD patients, 18 normal controls (NC). Fractional anisotropy (FA) values obtained from three...
Apolipoprotein E (APOE) ϵ4 allele is a very well-known genetic risk factor of Alzheimer's disease (AD). Recent histopatholgical and MRI studies in patients with AD demonstrated alterations white matter, as well gray matter. This study aimed to investigate possible associations between cerebral matter changes APOE genotype early stage AD. The authors used Diffusion Tensor Imaging (DTI) explore detect pathological processes. 26 participants were included the which 13 carriers age-, gender-,...
서 론 자가조절진정(patient-controlled sedation)은 환자 스스로 진정 제를 만족할만한 진정상태에 이를 때까지 투여하면서 가 원하는 정도의 진정상태를 유지하는 방법으로 개개인의 약동학적 차이를 극복할 수 있고 불안 및 의 식상태를 조절할 있는 장점이 있으며 과량투여로 인한 의식소실을 예방할 진정제 투여량 감소효과가 있 다. 1) 그러나 진정도입시간이 늦고, 적절한 진정상태 도달이 어려운 경우가 있으며, 수술에 대한 망각 효과가 적다.따 라서 자가조절진정 시 일회주입량의 선택과 대상환 자의 선별이 중요하며, 일회주입법과 더불어 지속주입법을 병용하는 방법도 고려해 보아야 한다. 2) 또한 진정깊이를 조절하여 비교적 안전하기는 하나 간혹 과진정 상태로 도입되어 눈꺼풀반사도 소실되는 있으므로, 수술 중 진정정도의 측정이 필수적이다. 3)청각유발전위(auditory evoked potential
Abstract BACKGROUND: Although fine airborne particulate matter (PM) has been known to play a role in many human diseases, there is no direct evidence that therapeutic drugs or proteins can diminish PM-induced diseases. Nevertheless, studies examining the negative control mechanisms of diseases are critical develop novel medications. RESULTS: We found CLB2.0, surrogate for PM, induced production multiple cytokines alter airway inflammation. Interestingly, deletion each PDZ domain ZO-1 protein...