A5049226671- Hematopoietic Stem Cell Transplantation
- Zebrafish Biomedical Research Applications
- Angiogenesis and VEGF in Cancer
- Single-cell and spatial transcriptomics
- Acute Myeloid Leukemia Research
- Immune cells in cancer
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Atherosclerosis and Cardiovascular Diseases
- Clusterin in disease pathology
- Cancer Genomics and Diagnostics
- Congenital heart defects research
- Pleural and Pulmonary Diseases
- Mesenchymal stem cell research
- Birth, Development, and Health
- 3D Printing in Biomedical Research
- Platelet Disorders and Treatments
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
- Cell Adhesion Molecules Research
- Renal and related cancers
- Epigenetics and DNA Methylation
- Pulmonary Hypertension Research and Treatments
- Cancer-related molecular mechanisms research
- Erythrocyte Function and Pathophysiology
Cornell University
2016-2023
Weill Cornell Medicine
2017-2023
Howard Hughes Medical Institute
2016
Abstract Although kidney parenchymal tissue can be generated in vitro, reconstructing the complex vasculature of remains a daunting task. The molecular pathways that specify and sustain functional, phenotypic structural heterogeneity are unknown. Here, we employ high-throughput bulk single-cell RNA sequencing non-lymphatic endothelial cells (ECs) to identify dictate vascular zonation from embryos adulthood. We show manifests vascular-specific signatures expressing defined transcription...
Chronic obstructive pulmonary disease (COPD) is marked by airway inflammation and airspace enlargement (emphysema) leading to airflow obstruction eventual respiratory failure. Microvasculature dysfunction associated with COPD/emphysema. However, it not known if abnormal endothelium drives COPD/emphysema pathology and/or correcting endothelial has therapeutic potential. Here, we show the centrality of cells pathogenesis in human tissue using an elastase-induced murine model emphysema....
Angiocrine factors, such as Notch ligands, supplied by the specialized endothelial cells (ECs) within bone marrow and splenic vascular niche play an essential role in modulating physiology of adult hematopoietic stem progenitor (HSPCs). However, relative contribution various specifically jagged-2, to homeostasis HSPCs is unknown. Here, we show that under steady state, jagged-2 differentially expressed tissue-specific beds, but its expression induced niches after myelosuppressive injury. We...
The hematopoietic niche is a supportive microenvironment composed of distinct cell types, including specialized vascular endothelial cells that directly interact with stem and progenitor (HSPCs). molecular factors specify orchestrate HSPC homeostasis remain largely unknown. Using multi-dimensional gene expression chromatin accessibility analyses in zebrafish, we define conserved signature cis-regulatory landscape are unique to sinusoidal the niche. enhancer mutagenesis transcription factor...
Abstract Successful expansion of bone marrow (BM) hematopoietic stem and progenitor cells (HSPCs) would benefit many HSPC transplantation gene therapy/editing applications. However, current technologies have been limited by a loss multipotency self-renewal properties ex vivo. We hypothesized that an vivo vascular niche provide prohematopoietic signals to expand HSPCs while maintaining self-renewal. To test this hypothesis, BM autologous CD34+ were expanded in endothelial cell (EC) coculture...
Transplanting vascular endothelial cells (ECs) to support metabolism and express regenerative paracrine factors is a strategy treat vasculopathies promote tissue regeneration. However, transplantation strategies have been challenging develop, because ECs are difficult culture little known about how direct them stably integrate into vasculature. Here we show that only amniotic could convert maintain EC gene expression. Even so, these converted perform sub-optimally in studies. Constitutive...
Abstract Objective Endothelial cells form vascular beds in all organs and are exposed to a range of mechanical forces that regulate cellular phenotype. We sought determine the role endothelial luminal surface stiffness tissue‐specific mechanotransduction laminar shear stress microvascular mouse arachidonic acid mediating this response. Methods Microvascular were subjected at 4 dynes/cm 2 for 12 hours parallel plate flow chambers enabled real‐time optical microscopy atomic force measurements...
Abstract The hematopoietic niche is a supportive microenvironment comprised of distinct cell types, including specialized vascular endothelial cells that directly interact with stem and progenitor (HSPCs). molecular factors specify orchestrate HSPC homeostasis remain largely unknown. Using multi-dimensional gene expression chromatin accessibility analyses, we define conserved signature cis -regulatory landscape unique to sinusoidal in the niche. enhancer mutagenesis transcription factor...