A5062218482- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Structural Anomalies and Repair
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Cardiomyopathy and Myosin Studies
- Cardiac Fibrosis and Remodeling
- Cardiovascular Disease and Adiposity
- Extracellular vesicles in disease
- Cardiac electrophysiology and arrhythmias
- Cardiac Valve Diseases and Treatments
- Cardiovascular Effects of Exercise
- Cardiac Imaging and Diagnostics
- Congenital heart defects research
- Chemotherapy-induced cardiotoxicity and mitigation
- Innovation and Socioeconomic Development
- Advanced Machining and Optimization Techniques
- Signaling Pathways in Disease
- Pancreatic function and diabetes
- Lipoproteins and Cardiovascular Health
- Cancer-related Molecular Pathways
- Cardiovascular Function and Risk Factors
- Viral Infections and Immunology Research
- Diabetes Treatment and Management
Stanford University
2018-2024
Cardiovascular Institute of the South
2018-2024
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2014-2018
Badalona Serveis Assistencials
2015
Hospital Universitari Germans Trias i Pujol
2014-2015
Sacubitril/Valsartan, proved superiority over other conventional heart failure management treatments, but its mechanisms of action remains obscure. In this study, we sought to explore the mechanistic details for Sacubitril/Valsartan in and post-myocardial infarction remodeling, using an silico, systems biology approach. Myocardial transcriptome obtained response myocardial swine was analyzed address post-infarction ventricular remodeling. Swine hits were mapped their human equivalents...
Abstract Aims Genetic dilated cardiomyopathy (DCM) is a leading cause of heart failure. Despite significant progress in understanding the genetic aetiologies DCM, molecular mechanisms underlying pathogenesis familial DCM remain unknown, translating to lack disease-specific therapies. The discovery novel targets for treatment was sought using phenotypic sceening assays induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) that recapitulate disease phenotypes vitro. Methods and...
Background: Phospholamban (PLN) is a critical regulator of calcium cycling and contractility in the heart. The loss arginine at position 14 PLN (R14del) associated with dilated cardiomyopathy high prevalence ventricular arrhythmias. How R14 deletion causes poorly understood, there are no disease-specific therapies. Methods: We used single-cell RNA sequencing to uncover R14del disease mechanisms human induced pluripotent stem cells (hiPSC-CMs). both 2-dimensional 3-dimensional functional...
Abstract Cardiac tissue engineering, which combines cells and supportive scaffolds, is an emerging treatment for restoring cardiac function after myocardial infarction (MI), although, the optimal construct remains a challenge. We developed two engineered grafts, based on decellularized scaffolds from pericardial tissues repopulated them with adipose mesenchymal stem (ATMSCs). The structure, macromechanical micromechanical scaffold properties were preserved upon decellularization...
Engineered bioimplants for cardiac repair require functional vascularization and innervation proper integration with the surrounding myocardium. The aim of this work was to study nerve sprouting neovascularization in an acellular pericardial-derived scaffold used as a myocardial bioimplant. To end, 17 swine were submitted infarction followed by implantation decellularized human scaffold. After 30 days, animals sacrificed hearts analyzed hematoxylin/eosin Masson's Gallego's modified trichrome...
Abstract Considerable research has been dedicated to restoring myocardial cell slippage and limiting ventricular remodeling after infarction (MI). We examined the ability of a three-dimensional (3D) engineered fibrin patch filled with human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) induce recovery cardiac function MI. The UCBMSCs were modified coexpress luciferase fluorescent protein reporters, mixed fibrin, applied as an adhesive, viable construct (fibrin-cell patch)...
Dilated cardiomyopathy (DCM) is a common cause of heart failure and sudden cardiac death. It has been estimated that up to half DCM cases are hereditary. Mutations in more than 50 genes, primarily autosomal dominant, have reported. Although rare, recessive mutations thought contribute considerably DCM, especially young children. Here we identified novel mutation the striated muscle enriched protein kinase (SPEG, p. E1680K) gene family with nonsyndromic, early onset DCM. To ascertain...
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a familial cardiac disease associated with arrhythmias and an increased risk of sudden death. Mutations in the desmosome gene Plakophilin-2, PKP2, lead to reduction PKP2 protein collapse desmosomes that known compromise contractility electrical stability cardiomyocytes. Our previous studies demonstrated efficacy adeno-associated virus 9 (AAV9)-mediated restoration expression specific knock-out mouse model Pkp2 revealed profound...
Abstract Cardiac cells are subjected to mechanical and electrical forces, which regulate gene expression cellular function. Therefore, in vitro electromechanical stimuli could benefit further integration of therapeutic into the myocardium. Our goals were (a) study viability a tissue-engineered construct with cardiac adipose tissue-derived progenitor (cardiac ATDPCs) (b) examine effect electromechanically stimulated ATDPCs within myocardial infarction (MI) model mice for first time. at...
Abstract Cardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed function scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises scaffold decellularized human pericardium, green fluorescent protein-labeled porcine adipose tissue-derived progenitor (pATPCs), customized-design electrical spectroscopy (EIS) monitoring system. was evaluated...
Despite recent advances, myocardial infarction (MI) remains the leading cause of death worldwide. Pre-clinical animal models that closely mimic human MI are pivotal for a quick translation research and swine have similarities in anatomy physiology. Here, we compared coronary surgical ligation versus coil embolization swine.Fifteen animals were randomly distributed to undergo (n=7) or (n=8). We evaluated infarct size, scar fibrosis, inflammation, vascularization, cardiac function by magnetic...
The optimal cell lineage for cardiac-regeneration approaches remains mysterious. Additionally, electrical stimulation promotes cardiomyogenic differentiation of stimulated cells. Therefore, we hypothesized that conditioning cardiomyocyte progenitor cells (CMPCs) might enrich their cardiovascular potential. CMPCs were isolated from human adult atrial appendages, characterized, and electrically 7 14 days. Electrical modulated gene protein expression, increasing all cardiac markers....
Myocardial infarction (MI) remains a dreadful disease around the world, causing irreversible sequelae that shorten life expectancy and reduce quality of despite current treatment. Here, authors engineered cell-enriched myocardial graft, composed decellularized matrix refilled with adipose tissue-derived progenitor cells (EMG-ATDPC). Once applied over infarcted area in swine MI model, EMG-ATDPC improved cardiac function, reduced infarct size, attenuated fibrosis progression, promoted...
MicroRNAs are small, noncoding RNAs that play a key role in gene expression. Accumulating evidence suggests aberrant microRNA expression contributes to the heart failure (HF) phenotype; however, underlying molecular mechanisms not well understood. A better understanding of action microRNAs could potentially lead targeted therapies halt progression or even reverse HF.We found microRNA-152 (miR-152) was upregulated failing human and experimental animal models HF. Transgenic mice with...
Cell-based strategies to regenerate injured myocardial tissue have emerged over the past decade, but optimum cell type is still under scrutiny. In this context, human adult epicardial fat surrounding heart has been characterized as a reservoir of mesenchymal-like progenitor cells (cardiac ATDPCs) with potential clinical benefits. However, additional data on possibility that these could trigger deleterious immune response following implantation are needed. Thus, in presented study, we took...
Abstract BACKGROUND There is growing evidence that pathogenic mutations do not fully explain hypertrophic (HCM) or dilated (DCM) cardiomyopathy phenotypes. We hypothesized if a patient’s genetic background was influencing this should be detectable as signatures in gene expression. built biobank resource for interrogating personalized genotype phenotype relationships human cell lines. METHODS recruited 308 diseased and control patients our stem biobank. successfully reprogrammed PBMCs...
Abstract Idiopathic dilated cardiomyopathy ( IDCM ) is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low‐density lipoprotein receptor‐related protein 1 LRP 1) has been linked to the underlying molecular basis disease. This study compared circulating levels soluble sLRP in patients healthy controls elucidated whether exported out myocardium through extracellular vesicles EV s) gain better understanding pathogenesis α chain expression was...
The combination of biomatrices and induced pluripotent stem cell (iPSC) derivatives to aid repair myocardial scar formation may soon become a reality for cardiac regenerative medicine. However, the tumor risk associated with residual undifferentiated cells remains an important safety concern iPSC-based therapies. This is not satisfactorily addressed in xenotransplantation, which requires immune suppression transplanted animal. In this study, we assessed transplanting iPSCs allogeneic...
Mechanical conditioning is incompletely characterized for stimulating therapeutic cells within the physiological range. We sought to unravel mechanism of action underlying mechanical adipose tissue-derived progenitor (ATDPCs), both in vitro and silico. Cardiac ATDPCs, grown on 3 different patterned surfaces, were mechanically stretched 7 days at 1 Hz. A custom-designed, magnet-based, stimulator device was developed apply ~10% stretching monolayer cell cultures. Gene protein analyses...