Jack C. D. Lee
A5020904340- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- COVID-19 epidemiological studies
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Metabolism and Genetic Disorders
- Genomics and Rare Diseases
- Mycobacterium research and diagnosis
- Viral Infections and Immunology Research
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Pituitary Gland Disorders and Treatments
- Immune responses and vaccinations
- Epilepsy research and treatment
- Adrenal Hormones and Disorders
- vaccines and immunoinformatics approaches
- Plant Virus Research Studies
- Inflammasome and immune disorders
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Hormonal Regulation and Hypertension
Great Ormond Street Hospital for Children NHS Foundation Trust
2021-2023
University of Leicester
2023
Great Ormond Street Hospital
2023
University College London
2023
Imperial College London
2022
Quadram Institute
2022
UK Research and Innovation
2021
National Institute for Health Research
2021
MRC Biostatistics Unit
2021
Public Health England
2021
Abstract Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis children have been reported worldwide, including 278 the UK 1 . Here we report an investigation 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination genomic, transcriptomic, proteomic immunohistochemical methods. We detected high levels adeno-associated virus 2 (AAV2) DNA liver, blood, plasma or stool from 27 28 cases....
Summary Since the first reports of hepatitis unknown aetiology occurring in UK children, over 1000 cases have been reported worldwide, including 268 UK, with majority younger than 6 years old. Using genomic, proteomic and immunohistochemical methods, we undertook extensive investigation 28 136 control subjects. In five who underwent liver transplantation, detected high levels adeno-associated virus 2 (AAV2) explanted livers. AAV2 was also at blood from 10/11 non-transplanted cases. Low...
Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this routinely in clinical service, processing 178 over the past 2.5 years that adheres to ISO:15189 standards. have successfully used diagnose multiple cases encephalitis hepatitis.
Abstract Since the first reports of hepatitis unknown aetiology occurring in UK children, over 1000 cases have been reported worldwide, including 268 UK, with majority younger than 6 years old. Using genomic, proteomic and immunohistochemical methods, we undertook extensive investigation 28 136 control subjects. In five who underwent liver transplantation, detected high levels adeno-associated virus 2 (AAV2) explanted livers. AAV2 was also at blood from 10/11 non-transplanted cases. Low...
ABSTRACT Background Atypical or complex paediatric neurological presentations are common clinical conundrums and often remain undiagnosed despite extensive investigations. This is particularly pronounced in immunocompromised patients. Here we show that metagenomics (CMg) a valuable adjunct diagnostic tool to be used by neuro-infection multidisciplinary teams (MDTs). Methods We included patients referred the Great Ormond Street Hospital MDT whom uncertainty remained, standardised...
Infections by several DNA viruses can severely impact outcomes in paediatric immunocompromised patients. Current testing, which is generally limited to singleplex qPCR assays, miss both common and rarer if they are not targeted.To evaluate the performance of Galileo Viral Panel (Galileo), a sample-to-result shotgun metagenomics platform for detection quantification 12 viruses, compared standard care assays.A clinical evaluation was carried out using 43 prospectively collected EDTA plasma...
Background: Atypical or complex paediatric neurological presentations are common clinical conundrums and often remain undiagnosed despite extensive investigations. This is particularly pronounced in immunocompromised patients. Here we show that metagenomics (CMg) a valuable adjunct diagnostic tool to be used by neuro-infection multidisciplinary teams (MDTs).Methods: We included patients referred the Great Ormond Street Hospital MDT whom uncertainty remained, standardised comprehensive set of...