A5081340453- Gene expression and cancer classification
- Genetics, Bioinformatics, and Biomedical Research
- Pharmacogenetics and Drug Metabolism
- Molecular Biology Techniques and Applications
- Ethics in Clinical Research
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- Bioinformatics and Genomic Networks
- Biomedical and Engineering Education
- Aquaculture Nutrition and Growth
- Alzheimer's disease research and treatments
- Marine Biology and Environmental Chemistry
- Effects and risks of endocrine disrupting chemicals
- Biomedical Ethics and Regulation
- Neuroscience and Neuropharmacology Research
- Reproductive biology and impacts on aquatic species
- Organometallic Compounds Synthesis and Characterization
- Biosensors and Analytical Detection
- Receptor Mechanisms and Signaling
- Genomics and Rare Diseases
- Law, AI, and Intellectual Property
- Distributed and Parallel Computing Systems
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
Rutgers Cancer Institute of New Jersey
2018
Rutgers, The State University of New Jersey
2018
Purdue University West Lafayette
1998-2014
Ohio Northern University
2011
Genomic Health (United States)
2001-2006
Johnson University
2005
Riverside Technology (United States)
2001
Pfizer (United States)
2000
To examine the utility and performance of 50mer oligonucleotide (oligonucleotide probe) microarrays, gene-specific probes were spotted along with PCR onto glass microarrays each probe type was evaluated. The specificity studied using target RNAs that shared various degrees sequence similarity. Sensitivity defined as ability to detect a 3-fold change in mRNA. No significant difference sensitivity between observed both had minimum reproducible detection limit ∼10 mRNA copies/cell. Specificity...
Summary Studies demonstrate that GRM , expressed by >60% of human melanomas, may be a therapeutic target. We performed phase II trial 100 mg PO bid riluzole, an inhibitor 1 signaling, in patients with advanced melanoma the primary endpoint response rate. Thirteen 1‐positive tumors were enrolled. No objective responses observed, and accrual was stopped. Stable disease noted six (46%) patients, one patient on study for 42 weeks. Riluzole well tolerated, fatigue (62%) as most common adverse...
Endocrine disrupting chemicals (EDCs) are known to contaminate aquatic environments and alter the growth reproduction of organisms. The objective this study was evaluate sensitivity utility fathead minnow (Pimephales promelas) early life-stages as a model measure effects estrogenic antiestrogenic EDCs on physiological gene expression endpoints relative reproduction. Embryos (<24-h postfertilization, hpf) were exposed potent estrogen (17α-ethinyl estradiol, EE(2) , 2, 10, 50 ng L(-1)); weak...
ABSTRACT Multiplex polymerase chain reaction (mPCR), the simultaneous amplification of two or more (PCR) products in same tube, can be applied to rapid detection pathogenic microorganisms food matrices. mPCR used test for multiple pathogens simultaneously virulence factors associated with a single pathogen. Heterogeneous such as Escherichia coli cannot only detected by but also differentiated pathotype serotype based on amplicon profiles. Sensitive ( ≤ 1 cfu/g) achieved if high concentration...
Abstract Recently, researchers have begun looking at changes in gene expression the fathead minnow ( Pimephales promelas ) after contaminant exposure as a way to develop biomarkers of and effects. However, bulk this research has been conducted on adults, with few studies focusing early life stages. Expression selected genes important growth, development, reproduction teleosts was quantified by quantitative polymerase chain reaction during different developmental time periods (from 0 28 d...
Abstract: The differentiated PC12 cell neuronal model was used to determine the effect of trimethyltin (TMT) on protein kinase C (PKC). Cells treated with 5–20 µ M TMT showed a partial and sustained PKC translocation within 30 min persisted over 24‐h period. treatment accompanied by low level down‐regulation 24 h, which small compared that produced phorbol esters. Confocal imaging cells translocates plasma membrane is blocked inhibitor chelerythrine (1 ). Phorbol myristate‐induced or...
The functional viability of cells can be evaluated using a number different assay determinants. One common involves exposing to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), which is converted intracellularly colored formazan precipitate and often used assess amyloid peptide-induced cytotoxic effects. MTT was employed evaluate the role endosomal uptake lysosomal acidification in peptide-treated differentiated PC12 cell cultures selective vacuolar-type (V-type) ATPase...
The fathead minnow Pimephales promelas serves as a model organism for assessing the effects of environmental contaminants on early life stage growth and development. Yet, utilization genomic tools has been hindered by lack genome sequence information known from this species. Utilizing published cDNA library sequences, authors used similarity to compare 4105 cDNAs isolated fry (<14 days old) with over 250 000 adult sequences derived whole body various tissue types. objectives computational...
Systems biology aims to understand biological systems on a comprehensive scale, such that the components make up whole are connected one another and work through dependent interactions. Molecular correlations comparative studies of molecular expression crucial establishing interdependent connections in biology. The existing software packages provide limited data mining capability. user must first generate visualization with preferred algorithm then upload resulting into package for graphic...
Abstract Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental DNA‐damaging agents regarded as risk factors for human disease, including lung and breast cancer. The biotransformation of PAHs to carcinogenic metabolites is mediated by the hydrocarbon receptor (AhR), which activates transcription at xenobiotic responsive elements (XREs = 5′‐GCGTG‐3′) found in promoter regions genes encoding detoxifying enzymes, CYP1A1 CYP1B1 . In this study, we wished identify novel biomarkers...
The rationale and overall system-wide behavior of a clinical genotyping information system (both DNA analysis data management) requires near-term, scalable approach, which is emerging in the focused implementation pharmacogenomics drug safety assurance. challenges to implementing successful are described, as how benefits focused, near-term for assessment assurance overcome logistical operational that perpetually hinder development societal-scale system. This based on premise application...
In this article, we present the Laboratory Inventory Network Application (LINA), a software system that assists research laboratories in keeping track of their collections biologically relevant materials. This open source application uses relational Microsoft Access database technology as back end and .NET front end. Preconstructed table templates are provided contain standardized customizable data fields. As new samples added to inventory, each is with unique laboratory identifier, which...
Abstract: Trimethyltin (TMT) is a potent neurotoxic compound that initiates delayed neuronal cell death. Previously we have shown TMT‐induced cytotoxicity associated with protein kinase C (PKC) translocation and activation. The present study investigates the mechanism underlying TMT‐stimulated PKC in PC12 cells. TMT exposure led to rapid increase intracellular levels of inositol 1,4,5‐trisphosphate (IP 3 ), product phospholipase (PLC). This was significantly decreased by pretreating cells...
The utilization of parallel processing in bioinformatics is a relatively new development, and wide spread use this domain has been limited by the availability applications that exploit architectures, as well historic lack IT training life sciences. Presented an evaluation several computing clusters using two different gene sequence search capable utilizing architecture (ParAlign mpiBLAST). objective study to evaluate utility these dedicated searching (developed for architectures) on systems....
The utilization of multi-core and multi-node parallel high performance computing (HPC) systems is growing rapidly to meet computational demands in the scientific arena. For example, exponential growth genomic data has outpaced increases single CPU clock speeds by 15-fold over last 20 years, placing great value on use processing bioinformatics. Fortunately, increased demand for architectures resulted decreased costs distributed components making these more affordable organizations...
Recent events in both the health care and research community have increased opportunities available for information technology systems integration professionals. In care, mandatory performance specifications electronic records set forth by United States federal government placed essentially all aspects of center stage within healthcare. Similarly scientific research, completion human genome 2001 has forced researchers to become dependent upon capabilities sciences convert genomic data into...