A5043688508- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Marine and coastal ecosystems
- Proteoglycans and glycosaminoglycans research
- Oceanographic and Atmospheric Processes
- Lysosomal Storage Disorders Research
- Invertebrate Immune Response Mechanisms
- Trypanosoma species research and implications
- Galectins and Cancer Biology
- Cellular transport and secretion
- Marine Biology and Ecology Research
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- Isotope Analysis in Ecology
- Polysaccharides and Plant Cell Walls
- Geology and Paleoclimatology Research
- Caveolin-1 and cellular processes
- Ocean Waves and Remote Sensing
- Sphingolipid Metabolism and Signaling
- Marine Toxins and Detection Methods
- Tropical and Extratropical Cyclones Research
- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
- Neurobiology and Insect Physiology Research
- Marine and fisheries research
University of Georgia
2015-2025
Tokyo Medical and Dental University
2017-2025
Medical College of Wisconsin
2018-2024
Cancer Research Center
2024
Japan Fisheries Research and Education Agency
2012-2023
Medical College of Wisconsin Cancer Center
2023
Piedmont Athens Regional
2021
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2012
National Institutes of Health
2012
Eisenhower Medical Center
2012
The structural diversity of glycoprotein N-linked oligosaccharides is determined by the expression and regulation glycosyltransferase activities availability appropriate acceptor/donor substrates. Cells in different tissues developmental stages utilize these control points to manifest unique glycan patterns response their surroundings. activity a Toll-like receptor, called Tollo/Toll-8, induces pattern incompletely defined, but neural specific, Drosophila embryo. Understanding full extent...
'Salt & Pepper' syndrome is an autosomal recessive condition characterized by severe intellectual disability, epilepsy, scoliosis, choreoathetosis, dysmorphic facial features and altered dermal pigmentation. High-density SNP array analysis performed on siblings first described with this detected four shared regions of loss heterozygosity (LOH). Whole-exome sequencing narrowed the candidate region to chromosome 2p11.2. Sanger confirmed a homozygous c.994G>A transition (p.E332K) in ST3GAL5...
Rapid and continued growth in the generation of glycomic data has revealed need for enhanced development basic infrastructure presenting interpreting these datasets a manner that engages broader biomedical research community. Early their growth, genomic proteomic fields implemented mechanisms assigning unique gene protein identifiers were essential organizing presentation enhancing bioinformatic approaches to extracting knowledge. Similar are currently absent from data. In order facilitate...
Gangliosides-sialylated glycosphingolipids-are the major glycoconjugates of nerve cells.The same four structures-GM1, GD1a, GD1b and GT1b-comprise great majority gangliosides in mammalian brains.They share a common tetrasaccharide core (Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-1′Cer) with one or two sialic acids on internal galactose zero (GM1 GD1b) (GD1a GT1b) α2-3-linked acid terminal galactose.Whereas genes responsible for sialylation are known, those have not been established vivo.We report that...
Abstract Human microbiome composition is closely tied to health, but how the host manages its microbial inhabitants remains unclear. One important, understudied, factor natural environment: mucus, which contains gel-forming glycoproteins (mucins) that display hundreds of glycan structures with potential regulatory function. Leveraging a tractable culture-based system study mucins influence oral communities, we found mucin glycans enable coexistence diverse microbes, while resisting...
Appropriate glycoprotein O-glycosylation is essential for normal development and tissue function in multicellular organisms. To comprehensively assess the developmental functional impact of altered O-glycosylation, we have extensively analyzed non-glycosaminoglycan, O-linked glycans expressed Drosophila embryos. Through multidimensional mass spectrometric analysis released from glycoproteins by beta-elimination, detected novel as well previously reported O-glycans that exhibit...
Robust quantification is an essential component of comparative -omic strategies. In this regard, glycomics lags behind proteomics. Although various isotope-tagging and direct methods have recently enhanced glycan analysis, a cell culture labeling strategy, that could provide for the advantages SILAC provides proteomics, has not been described. Here, we report development IDAWG, Isotopic Detection Aminosugars With Glutamine, incorporation differential mass tags into glycans cultured cells....
Glycosylation is a dynamic post-translational modification that changes during the development and progression of various malignancies. During oncogenesis breast carcinoma, glycosyltransferase known as N-acetylglucosaminyltransferase Va (GnT-Va) transcript levels activity are increased due to activated oncogenic signaling pathways. Elevated GnT-V leads β(1,6)-branched N-linked glycan structures on glycoproteins can be measured using specific carbohydrate binding protein or lectin L-PHA....
A game of tag: N-Glycans on the surface living cells were selectively tagged by exogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed a strain-promoted cycloaddition using biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect mechanisms that underlie altered glycoconjugate recycling storage in disease.
The main extracellular matrix binding component of the dystrophin-glycoprotein complex, α-dystroglycan (α-DG), which was originally isolated from rabbit skeletal muscle, is an extensively O-glycosylated protein. Previous studies have shown α-DG to be modified by both O-GalNAc- and O-mannose-initiated glycan structures. O-Mannosylation, accounts for up 30% reported O-linked structures in certain tissues, has been rarely observed on mammalian proteins. Mutations multiple genes encoding defined...
To address various fisheries science problems around Japan, the Japan Fisheries Research and Education Agency (FRA) has developed an ocean forecast system by combining circulation model based on Regional Ocean Modeling System (ROMS) with three-dimensional variational analysis schemes. This system, which is called FRA-ROMS, a basic essential tool for systematic conduct of science. The main aim FRA-ROMS to realistically simulate mesoscale variations over Kuroshio-Oyashio region. Here, in situ...
Fabry disease, the most common lysosomal storage affects multiple organs and results in a shortened life span. This disease is caused by deficiency of enzyme α-galactosidase A, which leads to glycosphingolipid accumulation many cell types. Neuropathic pain an early severely debilitating symptom patients with but cellular molecular mechanisms that cause are unknown. We generated rat model first nonmouse our knowledge. rats had substantial serum tissue α-galactosyl glycosphingolipids...
Alzheimer's disease (AD) is characterized by accumulation of misfolded proteins. Genetic studies implicate microglia, brain-resident phagocytic immune cells, in AD pathogenesis. As positive effectors, microglia clear toxic proteins, whereas as negative they release proinflammatory mediators. An imbalance these functions contributes to progression. Polymorphisms human CD33, an inhibitory microglial receptor, are linked susceptibility; higher CD33 expression correlates with increased risk....
Although the function of many glycoproteins in nervous system fruit flies is well understood, information about glycosylation profile and glycan attachment sites for such proteins scarce. In order to fill this gap facilitate analysis N-linked system, we have performed an extensive survey membrane-associated their N-glycosylation isolated from adult Drosophila brain. Following subcellular fractionation trypsin digestion, used different lectin affinity chromatography steps isolate...
After mating, many female mammals store a subpopulation of sperm in the lower portion oviduct, forming reservoir. The reservoir lengthens lifespan, regulates capacitation, controls polyspermy, and selects normal sperm. It is believed that bind to glycans on oviduct epithelium form reservoir, but specific adhesion molecules retain are unclear. Herein, using glycan array test 377 for their ability porcine sperm, we found two motifs common among all with sperm-binding ability: Lewis X...
Human siglecs are a family of 14 sialic acid-binding proteins, most which expressed on subsets immune cells where they regulate responses. Siglec-8 is selectively human allergic inflammatory cells-primarily eosinophils and mast cells-where engagement causes eosinophil apoptosis inhibits cell mediator release. Evidence supports model in bind to sialoglycan ligands target tissues resolve inflammation limit tissue damage. To identify Siglec-8-binding sialoglycans from airways, proteins...