A5077049090- Malaria Research and Control
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- vaccines and immunoinformatics approaches
- Travel-related health issues
- Hepatitis B Virus Studies
- Invertebrate Immune Response Mechanisms
- Vector-borne infectious diseases
- Digital Imaging for Blood Diseases
- Vibrio bacteria research studies
- Chemical Synthesis and Analysis
- Phagocytosis and Immune Regulation
- Influenza Virus Research Studies
- Complement system in diseases
- Biological Research and Disease Studies
- Genetics, Bioinformatics, and Biomedical Research
- Hepatitis Viruses Studies and Epidemiology
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Evolution and Genetic Dynamics
Institut Pasteur de Dakar
2016-2025
Institut Pasteur
2015
The level of spontaneous apoptosis in short-term lymphocyte cultures was evaluated different human immunodeficiency virus-negative groups either healthy control individuals or patients with clinical malaria. mean percentage found during a malaria attack significantly higher than sex- and age-matched controls. asymptomatic controls were degrees exposure to Plasmodium falciparum as reflected by their various levels specific anti-P. (immunoglobulin G M) antibodies. percentages apoptotic nuclei...
Abstract Background Assessment exposure and immunity to malaria is an important step in the fight against disease. Increased infection non-immune travellers under anti-malarial chemoprophylaxis, as well implementation of elimination programmes endemic countries, raises new issues that pertain these processes. Notably, monitoring has become more difficult individuals showing low antibody (Ab) responses or taking medications Plasmodium falciparum blood stages. Commonly available techniques...
In the progress towards malaria elimination, accurate diagnosis of low-density asymptomatic infections is critical. Low-density submicroscopic may act as silent reservoirs that maintain low-level residual transmission in community. Light microscopy, gold standard lacks sensitivity to detect parasitaemia. this study, presence and prevalence Plasmodium carriage were investigated estimate parasites reservoir among individuals living low areas Dielmo Ndiop, Senegal during dry season.A total...
Control efforts towards malaria due to Plasmodium falciparum significantly decreased the incidence of disease in many endemic countries including Senegal. Surprisingly, Kedougou (southeastern Senegal) P. remains highly prevalent and relative contribution other species global burden is very poorly documented, partly low sensitivity routine diagnostic tools. Molecular methods offer better estimate circulating a given area. A molecular survey was carried out document parasites region.A total...
Dramatic changes in transmission intensity can impact Plasmodium population diversity. Using samples from 2 distant time-points the Dielmo/Ndiop longitudinal cohorts Senegal, we applied a molecular barcode tool to detect parasite genotypes and complexity of infection that corresponded intensity. We observed striking statistically significant difference genetic diversity between populations. Furthermore, identified genotype Dielmo Ndiop previously Thiès, potentially implicating imported...
Malaria in Senegal is due essentially to infections by Plasmodium falciparum and, a lesser extent malariae and ovale. By the use of molecular methods, detection vivax has been recently reported region Kedougou, raising question appraisal its potential prevalence this setting. A retrospective serological study was carried out using 188 samples taken from 2010 2011 longitudinal school survey during which 48 asymptomatic children (9–11 years) were recruited. Four collections collected two...
Concurrent malaria and arbovirus infections are common represent an important public health concern in regions where both diseases endemic. The present study investigates the genetic diversity complexity of Plasmodium falciparum infection concurrent malaria-arbovirus Kedougou region, southeastern Senegal. Parasite DNA was extracted from 60 to 27 sera samples collected P. isolates infected patients, respectively, followed by PCR-genotyping targeting msp-1 (block2) msp-2 (block3) allelic...
Despite significant progress in malaria control over the past twenty years, remains a leading cause of child morbidity and mortality Tropical Africa. As most patients do not consult any health facility much uncertainty persists about true burden disease range individual differences susceptibility to malaria.
Although the burden of malaria has significantly declined in recent years sub-Saharan Africa through widespread use long-lasting insecticide treated bed-nets (LLINs) and artemisinin-based combination therapy, resurgence is observed some settings after several LLINs use. This study aimed to assess if remains protective against during a period morbidity Dielmo, rural village Senegal.In July 2008, were offered all villagers lately 2011, renewed. A longitudinal was conducted between July, 2010...
Genetic analyses of the malaria parasite population and its temporal spatial dynamics could provide an assessment effectiveness disease control strategies. The genetic diversity Plasmodium falciparum has been poorly documented in Senegal, limited data are available from Kedougou Region. This study examines variation complexity P. infections acute febrile patients Kedougou, southeastern Senegal. A total 263 sera presenting with illness attending health facilities between July 2009 2013 were...
ABSTRACT Antibodies against three long synthetic peptides (LSPs) derived from the glutamate-rich protein (GLURP) of Plasmodium falciparum were analyzed in cohorts Liberia, Ghana, and Senegal. Two overlapping LSPs, LR67 LR68, are relatively conserved N-terminal nonrepeat region (R0), third, LR70, is R2 repeat region. A high prevalence antibody responses to each LSP was observed all areas endemic infection. Levels cytophilic immunoglobulin G (IgG) antibodies both GLURP regions significantly...
In the southeastern Senegal, report of Plasmodium vivax infections among febrile patients in Kedougou constitutes a new emerging health problem. Samples from 48 asymptomatic schoolchildren sampled twice year over 2 years were used to explore reservoir P. parasite this region. Both Duffy genotyping and species diagnostic assays performed. PCR detected genomic DNA 38.5% (74/192) samples. Pure falciparum identified 79.7% (59/74) 20.3% (15/74) samples, respectively. All classified as...
Malaria is a leading cause of mortality and morbidity in tropical countries, especially sub-Saharan Africa. In Senegal, control plan implemented the beginning 2000s has enabled substantial reduction due to malaria. However, eradication malaria requires vaccine that protects against Plasmodium falciparum deadliest species parasite causes this disease. characterized by an extensive genetic diversity makes development challenging. study, P. isolates was analysed from asymptomatic children...
Liver-stage antigen 3 (LSA-3) is a new vaccine candidate that can induce protection against Plasmodium falciparum sporozoite challenge. Using series of long synthetic peptides (LSP) encompassing most the 210-kDa LSA-3 protein, study antigenicity this protein was carried out in 203 inhabitants from villages Dielmo (n = 143) and Ndiop 60) Senegal (the level malaria transmission differs these two villages). Lymphocyte responses to each individual peptide were recorded, some at high prevalences...
Introduction Submicroscopic Plasmodium infections are common in malaria endemic countries, but very little studies have been done Senegal. This study investigates the genetic diversity and complexity of submicroscopic P. falciparum among febrile patients low transmission areas Materials methods Hundred fifty blood samples were collected from individuals living Dielmo Ndiop (Senegal) between August 2014 January 2015, tested for microscopic sub-microscopic characterized their using msp-1 msp-2...
Coordinated scaled-up malaria control interventions have substantially contributed to the dramatic decrease of malaria-related morbidity and mortality in several endemic countries, including Senegal. However, impacts a given intervention on vector parasite populations, acquired immunity, disease burden remain very poorly documented largely due lack continuous surveys. This study took advantage sera bank established as part Dielmo longitudinal project investigate dynamics IgG antibody...
A detailed understanding of the contribution asymptomatic Plasmodium reservoir to occurrence clinical malaria at individual and community levels is needed guide effective elimination interventions. This study investigated relationship between falciparum carriage subsequent episodes in Dielmo Ndiop villages Senegal.The used a total 2792 venous capillary blood samples obtained from individuals datasets collected 2013 2016. Mapping, spatial clustering infections, risk analysis were performed...
The antibody-dependent respiratory burst (ADRB) assay is a sensitive isoluminol-based chemiluminescence (CL) functional designed to assess the capacity of opsonizing antibodies against merozoites induce neutrophil burst. ADRB was shown measure protective immunity malaria in endemic areas, but needed further improvement ensure better sensitivity and reproducibility. Here, we adjusted parameters such as freezing–thawing procedure merozoites, merozoites's concentration buffer solution's pH,...
In low malaria transmission areas, the elimination of disease has been hampered partly by existence a reservoir subpatent Plasmodium falciparum infections within communities. This reservoir, often undetected, serves as source parasites and contributes to ongoing clinical cases.
Abstract Background Combined malaria control interventions have reduced mortality rate, number of clinical cases and parasite prevalence across Africa between 2000 2015. As a consequence, Plasmodium infections become mostly asymptomatic often sub-microscopic in many endemic areas. The present study aims to evaluate the contribution P. falciparum carriage on antibody responses against antigens. Methods A total 353 samples from cross-sectional sampling conducted Ndiop (Senegal) 2016 were...