A5047516752- Malaria Research and Control
- Mosquito-borne diseases and control
- Complement system in diseases
- Parasites and Host Interactions
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis B Virus Studies
- Travel-related health issues
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Immunodeficiency and Autoimmune Disorders
- Vector-borne infectious diseases
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Invertebrate Immune Response Mechanisms
- Viral Infections and Outbreaks Research
- Hepatitis Viruses Studies and Epidemiology
- vaccines and immunoinformatics approaches
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Leprosy Research and Treatment
- Viral Infections and Immunology Research
- Drug Transport and Resistance Mechanisms
Centre Pasteur du Cameroun
2022-2025
Institut Pasteur de Dakar
2010-2023
Modèles Insectes de l'Immunité Innée
2023
Cheikh Anta Diop University
2015
Institut Pasteur
1994-2014
Centre National de la Recherche Scientifique
2004
Institut de Recherche pour le Développement
2004
Centre Hospitalier Régional Universitaire de Brest
2003
Institut Pasteur de la Guyane
1989-1998
University of Guyana
1993
Effective vaccines to combat malaria are urgently needed, but have proved elusive in the absence of validated correlates natural immunity. Repeated blood stage infections induce antibodies considered be main arbiters protection from pathology, their essential functions remained speculative.This study evaluated antibody dependent respiratory burst (ADRB) activity polymorphonuclear neutrophils (PMN) induced by Plasmodium falciparum merozoites and sera two different African endemic populations,...
Abstract We recently developed an efficient strategy based on a fully synthetic dendrimeric carbohydrate display (multiple antigenic glycopeptide; MAG) to induce anticarbohydrate antibody responses for therapeutic vaccination against cancer. Here, we show the superior efficacy of MAG over traditional keyhole limpet hemocyanin glycoconjugate elicit IgG response tumor-associated Tn antigen. highlight influence aglyconic carrier elements such tumor antigen their recognition by immune system....
Antibodies to Plasmodium falciparum C-terminal merozoite surface protein 1 (PfMSP-1p19) have been correlated with protection against malaria, but this association may apply many antigens. To address question, we conducted a prospective serological study of 205 individuals in an active 5-month clinical survey Senegalese village where malaria is mesoendemic. Before the 2000 rainy season, antibody responses specific for recombinant baculovirus PfMSP-1p19 or extracts were compared 2 vitro...
Pro-inflammatory cytokines induced by glycosylphosphatidylinositols (GPIs) of Plasmodium falciparum contribute to malaria pathogenesis and hence, the naturally acquired anti-GPI antibody thought provide protection against severe (SM) neutralizing stimulatory activity GPIs. In previous studies, levels increased with age in parallel development immunity, high antibodies were associated mild (MM) cases. present study, relationship between pro-inflammatory IgG responses, parasitemia, clinical...
Control efforts towards malaria due to Plasmodium falciparum significantly decreased the incidence of disease in many endemic countries including Senegal. Surprisingly, Kedougou (southeastern Senegal) P. remains highly prevalent and relative contribution other species global burden is very poorly documented, partly low sensitivity routine diagnostic tools. Molecular methods offer better estimate circulating a given area. A molecular survey was carried out document parasites region.A total...
SUMMARY We have previously shown in mice that antibodies can be induced to synthetic malaria peptides conjugated mycobacterial antigens, such as purilied protein derivative (PPD) or heal shock proteins (hsp), and given the absence of adjuvants after a previous priming with bacille Calmette-Guerin (BCG). In present study we investigated this model immunization non-human primates. Saimiri sciureus monkeys. Monkeys primed BCG subcutaneously then immunized Plasmodium falciparum sporozoite...
The squirrel monkey Saimiri sciureus is one of the World Health Organization recommended experimental models Plasmodium falciparum blood stage infection. Anti-malaria antibodies developed by this host after a drug-controlled infection play an important part in acquired protection against P. stages. Furthermore, use two anti-Saimiri immunoglobulin (Ig) monoclonal (mAb) has permitted differentiation between protective (mAb 3A2/G6) and non-protective 3E4/H8) antibodies, as shown transfer...
Numerous Plasmodium falciparum antigens elicit humoral responses in humans living endemic areas. Use of multiplex assays is a convenient approach to monitor the antibody response against multiple antigens, but integrate assay-derived data with datasets, generated previously using ELISA, comparative studies are needed. This work compares two P. monitored both technologies. The IgG merozoite surface protein-1 PfMSP1p19 and PF13-DBL1α1 domain Erythrocyte Membrane Protein1, expressed by...
Advances in malaria control have reduced the burden of disease resulting from exposure to parasite infections. The consequences on naturally acquired immunity are unclear. A magnetic bead-based immunoassay (MBA) assess antibody levels populations living endemic areas was previously evaluated. In this study, effect clinical attacks analysed three sentinel sites Ivory Coast. Recombinant proteins or peptides derived liver blood stage antigens Plasmodium falciparum (CSP, LSA141, LSA3, SALSA,...
Summary Anticardiolipin (ACA), anti-β2 glycoprotein I (β2GPI), and antiprothrombin antibodies of IgG IgM classes were quantitated by enzyme-linked immunosorbent assays in 176 untreated leprosy patients across the histopathological spectrum. Positivity rates ranged from 21% (IgG ACA) to 30% (IgM anti-prothrombin) versus 4% healthy controls (p <10-2 10-3). Levels anti-β2GPI ACA significantly higher lepromatous multibacillary patient subgroups. IgG3 was most common subclass reactive both...
Malaria in Senegal is due essentially to infections by Plasmodium falciparum and, a lesser extent malariae and ovale. By the use of molecular methods, detection vivax has been recently reported region Kedougou, raising question appraisal its potential prevalence this setting. A retrospective serological study was carried out using 188 samples taken from 2010 2011 longitudinal school survey during which 48 asymptomatic children (9–11 years) were recruited. Four collections collected two...
Background Plasmodium falciparum merozoite surface protein 5 (PfMSP5) is an attractive blood stage vaccine candidate because it both exposed to the immune system and well conserved. To evaluate its interest, we investigated association of anti-PfMSP5 IgG levels, in context responses two other conserved Ags PfMSP1p19 R23, with protection from clinical episodes malaria cross-sectional prospective studies different transmission settings. Methods Ndiop (mesoendemic) Dielmo (holoendemic) are...
Abstract Background Ongoing efforts to fight Plasmodium falciparum malaria has reduced in many areas, but new tools are needed monitor further progress, including indicators of decreasing exposure parasite infection. Sero-surveillance is considered promising exposure, transmission and immunity. Methods IgG responses three antigen biomarkers were evaluated a retrospective study involving: (i) surveys 798 asymptomatic villagers from 2 Senegalese endemic settings conducted before 2002 after the...
Despite significant progress in malaria control over the past twenty years, remains a leading cause of child morbidity and mortality Tropical Africa. As most patients do not consult any health facility much uncertainty persists about true burden disease range individual differences susceptibility to malaria.
According to the World Health Organization, surveillance for respiratory viruses with pandemic potential should be included in routine on alert zoonotic transmission. This study reports data from of influenza A/H5, A/H7 and MERS-Coronavirus Cameroon. A total 855 participants were enrolled. Of these, 11.7% positive none MERS-Coronavirus. Most (77.1%) enrolled within 5 days illness onset younger population under 2 years age (31.4%) was most represented. In terms clinical manifestations,...
Since 1971, Cameroon has experienced several outbreaks of cholera due to strains the seventh pandemic V. cholerae O1 El Tor (7PET) lineage originating from South Asia. Over last six years, more than 26,000 cases have been reported. The aim this study was perform a genomic characterisation isolates collected during recent in Cameroon. We investigated virulence, antimicrobial resistance (AMR) signatures and phylogenetic relationships between 164 clinical representative successive that were...
Recent control scale-up has reduced malaria in many areas but new tools are needed to monitor further progress, including indicators of decreasing exposure parasite infection. Although serology is considered a promising approach this regard, the serological impact interventions been so far studied using indirect quantification exposure. Cohort surveys concomitantly recording entomological and malariometric indices have conducted two Senegalese settings where supervised intensification...
ABSTRACT Cytophilic antibodies (Abs) play a critical role in protection against Plasmodium falciparum blood stages, yet little is known about the parameters regulating production of these Abs. We used an vitro culture system to study subclass distribution antigen (Ag)-specific immunoglobulin G (IgG) produced by peripheral mononuclear cells (PBMCs) from individuals exposed P. or unexposed individuals. PBMCs, cultivated with without cytokines and exogenous CD40/CD40L signals, were stimulated...
Identification of plasmodial antigens targeted by protective immune mechanisms is important for malaria vaccine development. Among functional assays, the neutrophil antibody-dependent respiratory burst (ADRB) induced opsonized Plasmodium falciparum merozoites has been correlated with acquired immunity to clinical in endemic areas, but target merozoite are unknown. Here, contribution antibodies conserved C-terminal domain P. surface protein-1 (PfMSP1p19) mediating ADRB was investigated sera...