and a cardiologist

Normally, the Wnt/beta-catenin pathway controls developmental processes and homeostasis, but abnormal activation of this is a frequent event during development cancer. The key mechanism in regulation amino-terminal phosphorylation beta-catenin, marking it for proteasomal degradation. Here we present small-molecule-based identification protein kinase C (PKC)-mediated beta-catenin as novel regulating pathway. We used cell-based chemical screen to identify A23187, which inhibits PKC was...

To evaluate the effects of three ABCG2 variants (Q141K, V12M and Q126X), which are known to have altered transport properties in vitro, on disposition lamivudine healthy subjects.To whether is a substrate ABCG2, intracellular accumulation vectorial 3H-lamivudine were determined MDCK-ABCG2 cells. The pharmacokinetic parameters compared among subjects with four different genotypes, including wild type (seven subjects), K141/K141 (six Q126/Stop126 (four subjects) M12/M12 (five after single oral...

Abstract A simple and rapid HPLC method using fluorescence detection was developed for determination of irbesartan in human plasma. Sample preparation accomplished through a deproteinization procedure with 0.4 mL acetonitrile containing 800 ng/mL losartan (internal standard), to 0.1 plasma sample. Chromatographic separation performed on Zorbax Xclipse XDB C 18 column (150 × 4.6 mm, i.d., 5 µm) at 40°C. An isocratic mobile phase, acetonitrile:0.1% formic acid (37:63, v/v), run flow‐rate 1.0...

Abstract We reported previously that protein kinase C‐α (PKC‐α) negatively regulates Wnt/β‐catenin signalling pathway. The current study explores the role of PKC‐α in regulation proliferation colon cancer cells, which contain aberrant up‐regulation intracellular β‐catenin. In tissue and an inverse correlation was observed between expression levels Activation inhibited β‐catenin response transcription by down‐regulation induced phosphorylation N‐terminal serine threonine residues...

Aim The primary objective of the present study was to evaluate pharmacokinetic and pharmacodynamic interactions between clopidogrel cilostazol in relation CYP2C19 CYP3A5 genotypes. Methods In a randomized, three‐way crossover study, 27 healthy subjects were administered (300 mg), (100 mg) or + orally. Plasma concentrations clopidogrel, their active metabolites (clopidogrel thiol metabolite, 3,4‐dehydrocilostazol 4″‐trans‐hydroxycilostazol), adenosine diphosphate‐induced platelet aggregation...

The hospital environment can be an important source for the transmission of pathogens, However, there are rare reports revealing contamination carbapenemase-producing enterobacterales (CPE) in environment. aim this study was to investigate presence CPE environments and their relation clinical strains.Environmental samples were collected from three tertiary university hospitals between June 2017 August 2019. environmental inoculated on CHROMagar™ KPC plates. A multiplex PCR sequencing used...

The primary objective of this study was to evaluate the effects Ginkgo biloba extracts (GBE) on pharmacokinetics cilostazol and its metabolites. secondary assess effect GBE pharmacodynamics cilostazol.A randomized, double-blind, two-way crossover conducted with 34 healthy Korean subjects. All subjects were given an oral dose (100 mg) plus (80 or placebo twice daily for 7 days. Plasma concentrations active metabolites (3,4-dehydrocilostazol 4'-trans-hydroxycilostazol) measured using liquid...

Salmonella is a major pathogen causing foodborne infections in humans. isolates are identified using biochemical and serological tests, including automated systems such as the VITEK2 system. However, there few reports on identification VITEK MS. Therefore, we aimed to evaluate usefulness of MALDI-TOF MS for identification. A total 1389 were ver3.0 or ver3.2. All confirmed by serotyping Kauffmann-White scheme, results compared with results. isolates, 66 serotypes, correctly at genus level...

Purpose: The aims of this study was to investigate the mutual pharmacokinetic interactions between steady-state atorvastatin and metformin effect food on fixed-dose combined (FDC) tablet extended release (XR). Subjects methods: Study 1, an open-labeled, fixed sequence, multiple-dose drug-drug interaction study, divided into 2 parts. Atorvastatin (40 mg) or (1,000 XR tablets were administered once daily via mono- co-therapy for 7 days. Plasma levels 2-OH-atorvastatin, quantitatively...

We evaluated the effect of CYP2C19 genotype over time on antiplatelet response clopidogrel in healthy subjects. Seventy subjects enrolled for a pharmacodynamic study and 22 pharmacokinetic took 300 mg first day 75 once daily six consecutive days. The with poor metabolizers (PM, N = 22) intermediate (IM, 37) had significantly delayed to inhibition platelet aggregation (IPA) compared extensive (EM, 33) (12 vs. 9 2 hours as median Tmax , P < .05) after clopidogrel. During maintenance doses...

Ye-Ji Lim, Eun-Young Cha, Hye-Eun Jung, Jong-Lyul Ghim, Su-Jun Lee, Kim* and Jae-Gook Shin* Department of Pharmacology PharmacoGenomics Research Center, Inje University College Medicine, Busan 614-735, Republic Korea, Clinical Pharmacology, Paik Hospital, Korea *Correspondence: J. G. Shin; Tel: +82-51-890-6709, Fax: +82-51-893-1232, E-mail: phshinjg@gmail.com; E. Y. Kim; +82-51-8908972, +82-51-895-6438, eykim@inje.ac.kr

Objective: This study compared the pharmacokinetic (PK) and safety profiles of a fixed-dose combination (FDC) formulation telmisartan S-amlodipine with those concomitant administration two drugs. Materials methods: was an open-label, randomized, crossover in healthy male Koreans. All subjects were administered FDC tablet containing 40 mg 5 also coadministered same dose both drugs given separately. The design included 14-day washout period between treatments. Blood samples collected up to 168...

Antiepileptic drugs (AEDs) can induce life-threatening severe cutaneous adverse reactions (SCARs) such as Stevens-Johnson syndrome (SJS), toxic epidermal necrolysis (TEN), and drug reaction with eosinophilia systemic symptoms (DRESS) syndrome.[1,2]Drug are usually caused by immunemediated mechanisms, the associations between culprit HLA alleles have been studied.[3]The relationship AED-induced SCARs has demonstrated for carbamazepine (CBZ) HLA-B*15:02 among Han Chinese.[4]CBZ-induced...

Hepatocyte nuclear receptor 4α (HNF4α) plays a central role in regulating human drug-metabolizing enzymes. Our previous study suggested that the newly identified polymorphism G60D HNF4α gene may decrease its downstream CYP2D6 activity Asians. To confirm this effect clinical setting, we carried out full pharmacokinetic of single oral dose substrate tolterodine 30 healthy Korean individuals (HNF4α wild type: n = 24; heterozygotes: 6) who were pregenotyped for CYP2D6. showed to be an...

Allopurinol-induced severe cutaneous adverse reactions (SCARs) such as Stevens-Johnson syndrome (SJS), toxic epidermal necrolysis (TEN), and drug reaction with eosinophilia systemic symptoms (DRESS) are reportedly associated the HLA-B*58:01 genotype. Three patients who developed SCARs after allopurinol administration were subjected to HLA-B genotyping lymphocyte activation test (LAT) evaluate genetic risk detect causative agent, respectively. All three given treat gout diagnosed DRESS...

Objective: The aim of this study was to compare the pharmacokinetics (PK) and safety profiles a fixed-dose combination (FDC) formulation fimasartan, amlodipine, rosuvastatin with co-administration two products by using replicated crossover design in healthy male subjects. Materials methods: This an open-label, randomized, three-sequence, three-period done because high coefficient variation PK parameter for that is, >30%. With 14 days washout period, FDC tablet containing 60 mg 10 20...

Objectives The monitoring of medication compliance in clinical trials is important but labor intensive. To check trials, a system was developed, and its technical feasibility evaluated. Methods consisted three parts: management part (clinical trial center database developed program), investigator (monitoring), participant (personal digital assistant [PDA] with barcode scanner). tested 20 participants for 2 weeks, Results This study that used PDA scanner, which sent reminder/warning messages,...

This study explored the association of pharmacogenomics with antipsychotic-induced amenorrhea in female patients schizophrenia. A total 89 schizophrenia aged 18–40 receiving consistent antipsychotics at a dose for more than 3 months were enrolled this study. Amenorrhea was defined as absence menstrual period or three periods row. Serum levels prolactin, estradiol, follicle-stimulating hormone, luteinizing and thyroid-stimulating hormone measured Cytochrome P450 2D6, dopamine receptor D2 (...

A common cause of drug hypersensitivity reactions is iodinated contrast media (ICM). ICM-induced had been considered to be a non-immunological reaction, but evidence for an immunological mechanism has increased recently. Thus, we evaluated whether HLA-A, -B, and -C alleles were associated with hypersensitivity. In total, 126 patients who underwent contrast-enhanced computed tomography studies through outpatient clinics at tertiary referral hospital between 2008 2012 assessed. Sixty-one...

Tenofovir and para-aminosalicylic acid (PAS) may be coprescribed to treat patients with concomitant infections of human immunodeficiency virus Mycobacterium tuberculosis bacteria. Both drugs are known have remarkable renal uptake transporter-mediated clearance. Owing the lack clinical studies on drug-drug interaction between 2 drugs, we conducted a translational study investigate effect PAS tenofovir pharmacokinetics (PK).Initially, studied in vitro interactions using stably transfected...