A5100747419- Bacterial Identification and Susceptibility Testing
- Agriculture, Soil, Plant Science
- Pneumonia and Respiratory Infections
- Genomics and Phylogenetic Studies
- Streptococcal Infections and Treatments
- Ecology and Conservation Studies
- Probiotics and Fermented Foods
- Mycobacterium research and diagnosis
- Diphtheria, Corynebacterium, and Tetanus
- Respiratory viral infections research
- Infective Endocarditis Diagnosis and Management
- Oral microbiology and periodontitis research
- Urinary Tract Infections Management
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Turfgrass Adaptation and Management
- Microbial infections and disease research
- Identification and Quantification in Food
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Listeria monocytogenes in Food Safety
- Infectious Diseases and Mycology
- Antimicrobial Resistance in Staphylococcus
- Job Satisfaction and Organizational Behavior
- Pneumocystis jirovecii pneumonia detection and treatment
Inje University
2009-2024
Semyung University
2017-2023
Inje University Busan Paik Hospital
2014-2019
University of La Verne
2017-2018
The Korean government established an antimicrobial resistance (AMR) surveillance system, compatible with the Global AMR Surveillance System (GLASS): Kor-GLASS. We describe results from first year of operation Kor-GLASS May 2016 to April 2017, comprising all non-duplicated clinical isolates major pathogens blood, urine, faeces and urethral cervical swabs six sentinel hospitals. Antimicrobial susceptibility tests were carried out by disk diffusion, Etest, broth microdilution agar dilution...
Gyu Ri Kim, Ph.D., Eun-Young Si Hyun Hae Kyung Lee, M.D., Jaehyeon Jong Hee Shin, Young Ree Sae Am Song, Joseph Jeong, Uh, Yu Dongeun Yong, Soo Sunjoo Ah Kyeong Seob Seok Hoon Namhee Ryoo, and Jeong Hwan M.D.. Ann Lab Med 2023;43:45-54. https://doi.org/10.3343/alm.2023.43.1.45
Background The isolation of nontuberculous mycobacteria ( NTM ) from clinical specimens has increased, and they now are considered significant opportunistic pathogens. aims this study were to develop a database interpretive criteria for identifying individual species. In addition, using isolates, we evaluated the usefulness 16S rRNA , hsp65 rpoB as target genes method. Methods sequences collected GenBank checked by manual inspection. Clinical isolates between 2005 2010 used DNA extraction,...
ABSTRACT Kocuria spp. are members of the Micrococcaceae family that frequently found in environment and on human skin. Few infections have been reported. We describe what appear to be first two cases marina peritonitis patients undergoing continuous ambulatory peritoneal dialysis.
The aims of this study were to compare several DNA extraction methods and 16S rDNA primers evaluate the clinical utility broad-range PCR in continuous ambulatory peritoneal dialysis (CAPD) culture fluids.Six type strains used as model organisms dilutions from 10(8) 10(0) colony-forming units (CFU)/mL for evaluation 5 primer pairs. Broad-range was applied 100 CAPD fluids, results compared with conventional results.There some differences between various sets regard detection limits. InstaGene...
Several factors contribute to differences in Streptococcus pneumoniae serotype distribution. We investigated the distribution and antimicrobial resistance of S. isolated between 2014 2016 Korea.We collected a total 1,855 isolates from 44 hospitals May 2016, analyzed serotypes by sequential multiplex PCR. each patient age, source clinical specimen, pattern.The most common were 11A (10.1%), followed 19A (8.8%), 3 (8.5%), 34 (8.1%), 23A (7.3%), 35B (6.2%). The major invasive (12.6%), (7.8%),...
An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20T, was isolated from pig faeces. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to family Erysipelotrichaceae, is most closely related Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides 5815T (92.2 %), Faecalicoccus acidiformans 15521T (90.2 %) Holdemanella biformis 5969T (89.6 %). Average...
Introduction . The aim of this study was to differentiate between Candida famata and guilliermondii correctly by using matrix-assisted laser desorption/ionization-time flight mass spectrometry (MALDI-TOF MS) gene sequencing. Methods Twenty-eight strains from blood cultures that had been identified as C. (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>N</mml:mi><mml:mo>=</mml:mo><mml:mn>25</mml:mn></mml:math>), /...
A lactic acid bacterium isolated from pig faeces was characterized using a polyphasic approach. The strain Gram-stain-positive, rod-shaped, and facultative anaerobic. Phylogenetic analysis of the 16S rRNA gene sequence indicated that isolate belonged to genus Lacticaseibacillus. multi-locus tree revealed formed sub-cluster adjacent Lacticaseibacillus kribbianus. main fatty acids were C16 : 0 C18 1ω9c. average nucleotide identity value, amino identity, genome-to-genome distance for YH-lacS6T...
Introduction . Streptococcus pneumoniae is an important pathogen with high morbidity and mortality rates. The aim of this study was to evaluate the distribution common serotypes antimicrobial susceptibility S. in Korea. Methods A total 378 pneumococcal isolates were collected from 2008 through 2014. We analyzed serotype for both invasive noninvasive isolates. Results Over 7 years, 3 (13.5%), 35 (10.8%), 19A (9.0%), 19F (6.6%), 6A (6.1%), 34 (5.6%) serotypes/serogroups. vaccine coverage rates...
Salmonella is one of the major causes food-borne infections. We investigated serotype distribution and antimicrobial resistance isolates collected in Korea between January 2016 December 2017. In total, 669 were from clinical specimens at 19 university hospitals. Serotyping was performed according to Kauffmann-White scheme, susceptibility tested using Sensititre EUVSEC plates or disk diffusion. Among strains, C (39.8%) B (36.6%) most prevalent serogroups. 51 serotypes identified, common S....
Two cocci-shaped, facultatively anaerobic, Gram-positive bacteria isolated from the faeces of a pig were designated as strains YH-aer221 T and YH-aer222. Analysis 16S rRNA gene sequences revealed that isolates most closely related to Aerococcus suis JCM 18035 with 96.6 % similarity. The multi-locus sequence tree formed sub-cluster adjacent A. . average nucleotide identity values for their 71.8 71.7 %, respectively; digital DNA–DNA hybridization 25.6 25.5 respectively. main fatty acids C 18 :...
Salmonella is a major pathogen causing foodborne infections in humans. isolates are identified using biochemical and serological tests, including automated systems such as the VITEK2 system. However, there few reports on identification VITEK MS. Therefore, we aimed to evaluate usefulness of MALDI-TOF MS for identification. A total 1389 were ver3.0 or ver3.2. All confirmed by serotyping Kauffmann-White scheme, results compared with results. isolates, 66 serotypes, correctly at genus level...
Rotavirus and norovirus are well-known causes of viral infectious diarrhea. There few reports on diarrhea caused by other viruses in Korea, although cases gastroenteritis attributable to increasing worldwide. The aims this study were detect various investigate their prevalence. A total 801 fecal specimens submitted a clinical microbiology laboratory for the detection diarrheal included. We sought rotavirus A/B/C, adenovirus, astrovirus, GI/GII, sapovirus, Aichi virus, human parechovirus,...
A lactic acid bacteria isolated from pig faeces was characterized using a polyphasic approach. Cells of the strain were Gram-stain-positive, rod-shaped and facultative anaerobic. Phylogenetic analysis 16S rRNA gene sequence indicated that isolate belonged to genus Lacticaseibacillus ; however, similarity other homologues within <98 %. Analysis housekeeping sequences ( pheS recA ) revealed formed sub-cluster adjacent absianus daqingensis . The main fatty acids is C 18 : 1 ω9 c 16 0 G+C...
Purpose The purpose of this paper is to examine the relationship between congruence employee and employer psychological contract fulfillment commitment. authors further studied how moderated by distributive justice. Design/methodology/approach conducted polynomial regression analyses with response surface methodology on two Korean samples. Findings Congruence was positively related affective commitment occupational Distributive justice these relationships. Research limitations/implications...
A rod-shaped, aerotolerant, Gram-stain-positive bacterium isolated from pig faeces was designated as strain YH-lim2214T. Analysis of the 16S rRNA gene sequence revealed that isolate most closely related to Limosilactobacillus pontis KCTC 25258T with 98.0 % similarity. The average nucleotide identity and amino acid values between YH-lim2214T Lm. were 81.4 81.3 %, respectively. major fatty acids C18 : 1 ω9c, summed feature 7 C16 0. cell-wall peptidoglycan type A4α l-Lys-d-Asp. genomic DNA G+C...
A short-rod-shaped, obligate anaerobic, Gram-positive bacterium isolated from the faeces of a pig was designated as strain YH-ols2223 T . Analysis 16S rRNA gene sequence revealed that isolate most closely related to Olsenella urininfantis KCTC 25755 with 97.7% similarity. The multi-locus tree formed sub-cluster within genus average nucleotide identity and digital DNA–DNA hybridization values for strains were 74.9–76.6% 20.8–22.5%, respectively. end products fermentation lactic acid acetic...
The aims of the current study were to investigate prevalence and molecular characteristics plasmid-mediated quinolone resistance (PMQR) genes from colonizing fecal organisms compare incidence subtype these according bacterial species hospital at five tertiary-care hospitals in Korea. A total 500 nonduplicated clinical isolates Enterobacteriaceae obtained specimens between March May 2008. PMQR (qnrA, qnrB, qnrS, aac(6')-Ib-cr, qepA) amplified by PCR confirmed direct sequencing products. 83...
This study was conducted to investigate the effect of different stockpiled periods on nutritive value and productivity mixed orchardgrass tall-fescue pasture (MOTP). experiment at Jeju (island), South Korea, from Sep. 2012 Nov. 2012. The DM yield MOTP had highest in treatment late August (2,983 kg/ha). yields swards were increased significantly by shortening period. CP 16.5% 18.16%, there did not appear be a consistent trend accordance with duration periods. NDF TDN content delay periods,...
The genus Hallella was described within Bacteroidaceae, and then reclassified Prevotellaceae based on its phenotypic phylogenetic description. It is associated with degradation of carbohydrate. However, some species have pathobiotic properties, are involved in infections chronic inflammatory disorders. Here, we used a polyphasic taxonomic approach to characterize the two strains: YH-C38T YH-C4B9b. A detailed metabolic analysis conducted compare novel isolates related strains Hallella....
Background Salmonella is an important pathogen that causes gastroenteritis and sepsis in humans. Recently, changes serotype prevalence increase antimicrobial resistance have been reported. This study investigated the distribution of serotypes determined susceptibility various strains. Methods We collected 113 isolates other than Typhi from 18 university hospitals 2015. The were identified by antisera O H according to Kauffman White scheme. Antimicrobial tests for 12 antibiotics performed...