A5040945709- Inflammasome and immune disorders
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Infant Nutrition and Health
- Research on Leishmaniasis Studies
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Diabetes and associated disorders
- Pancreatitis Pathology and Treatment
- Eosinophilic Disorders and Syndromes
- Chronic Myeloid Leukemia Treatments
- T-cell and B-cell Immunology
- Tryptophan and brain disorders
- Immune Response and Inflammation
- Adipokines, Inflammation, and Metabolic Diseases
- Antifungal resistance and susceptibility
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Endoplasmic Reticulum Stress and Disease
- IL-33, ST2, and ILC Pathways
- Seaweed-derived Bioactive Compounds
- Liver Diseases and Immunity
- Protease and Inhibitor Mechanisms
- Pelvic floor disorders treatments
- interferon and immune responses
- Menopause: Health Impacts and Treatments
University of California, San Diego
2019-2024
The University of Texas Southwestern Medical Center
2020-2023
Minerva Foundation
2022
Misgav Ladach
2022
Universidad de Salamanca
2022
National Jewish Health
2021
University of Colorado Denver
2021
Food and Nutrition Service
2020
Rady Children's Hospital-San Diego
2019
The University of Texas Health Science Center at Tyler
2017-2018
Infection with seasonal influenza A virus (IAV) leads to lung inflammation and respiratory failure, a main cause of death in influenza-infected patients. Previous experiments our laboratory indicate that Bruton's tyrosine kinase (Btk) plays substantial role regulating the region during acute injury mice; therefore, we sought determine if blocking Btk activity has protective effect influenza-induced inflammation. The inhibitor ibrutinib (also known as PCI-32765) was administered intranasally...
Nonalcoholic fatty liver disease (NAFLD) is the leading cause of chronic worldwide. The NLRP3 inflammasome, a platform for caspase-1 activation and release interleukin 1β, increasingly recognized in induction inflammation fibrosis during NAFLD. However, cell-specific contribution inflammasome NAFLD remains unknown.To investigate role hepatocytes, hepatic stellate cells (HSCs) myeloid cells, conditional Nlrp3 knock-out mouse was generated bred to Cre mice. Both acute injury models were used:...
Sterile inflammation is a central element in liver diseases. The immune response following injurious stimuli involves hepatic infiltration of neutrophils and monocytes. Neutrophils are major effectors inflammation, rapidly recruited to sites can augment the recruitment other leukocytes. NLRP3 inflammasome has been increasingly implicated severe fibrosis, cell death. In this study, role activation during fibrosis was investigated. Mouse models with neutrophil‐specific expression mutant were...
B cell development is a highly regulated process involving multiple differentiation steps, yet many details regarding this pathway remain unknown. Sequencing of patients with cell-restricted immunodeficiency reveals autosomal dominant mutations in TOP2B. TOP2B encodes type II topoisomerase, an essential gene required to alleviate topological stress during DNA replication and transcription, no previously known role development. We use Saccharomyces cerevisiae, knockin knockout murine models,...
The atherosclerotic process begins when vascular endothelial cells undergo pro-inflammatory changes such as aberrant activation to dysfunctional phenotypes and apoptosis, leading loss of integrity. Our laboratory has demonstrated that exposure mice second hand smoke triggers an increase in expression metalloproteinase-9. Further, metalloproteinase-9 released by smoke—activated leukocytes may propagate pro-atherogenic alterations cells. We have shown levels were increased the plasma from...
Macrophages contribute to the induction and resolution of inflammation play a central role in chronic low-grade cardiovascular diseases caused by atherosclerosis. Human milk oligosaccharides (HMOs) are complex unconjugated glycans unique human that benefit infant health act as innate immune modulators. Here, we identify HMO 3'sialyllactose (3'SL) natural inhibitor Toll-Like Receptor (TLR) 4-induced macrophages endothelium. Transcriptome analysis revealed 3'SL attenuates mRNA levels selected...
The few frontline antileishmanial drugs are poorly effective and toxic. To search for new this neglected tropical disease, we tested the activity of compounds in Medicines Malaria Venture (MMV) "Pathogen Box" against Leishmania amazonensis axenic amastigotes. Screening yielded six discovery with EC50 values from 50 to 480 nM. Concentration–response assays demonstrated that best hit, MMV676477, had mid-nanomolar cytocidal potency intracellular amastigotes, Trypanosoma brucei, Plasmodium...
BackgroundHoffman syndrome is a syndromic, inborn error of immunity due to autosomal-dominant mutations in TOP2B, an essential gene required alleviate topological stress during DNA replication and transcription. Although identified patients lead block B-cell development the absence circulating B cells, effect on natural killer (NK) cells was not previously examined.ObjectiveWe sought determine whether disease-associated TOP2B impact NK-cell function.MethodsUsing knockin murine model...
ABSTRACT Leishmania spp. are obligate intracellular parasites that must be internalized by phagocytic cells to evade immune responses and cause disease. The uptake of both promastigotes (insect-stage parasites) amastigotes (proliferative-stage in humans mice) phagocytes is thought mainly host cell driven, not parasite driven. Our previous work indicates Src- Abl-family kinases facilitate entry into macrophages pathogenesis murine cutaneous leishmaniasis. Here, we demonstrate spleen tyrosine...
Chronic obstructive pulmonary disease (COPD) is associated with severe chronic inflammation that promotes irreversible tissue destruction. Moreover, the most broadly accepted cause of COPD exposure to cigarette smoke. There no effective cure and significantly, mechanism behind development progression this remains unknown. Our laboratory has demonstrated Bruton’s tyrosine kinase (Btk) a critical regulator pro-inflammatory processes in lungs Btk controls expression matrix metalloproteinase-9...
Abstract Antisense oligonucleotides (ASOs) are a novel therapeutic strategy that targets specific gene and suppresses its expression. The cryopyrin-associated periodic syndromes (CAPS) spectrum of autoinflammatory diseases characterized by systemic tissue inflammation is caused heterozygous gain-of-function mutations in the nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain containing 3 (NLRP3) gene. aim this study was to investigate efficacy an Nlrp3-specific...
Abstract Choline is a vitamin-like nutrient that taken up via specific transporters and metabolized by choline kinase (ChoKα) which converts it to phosphocholine needed for de novo synthesis of phosphatidylcholine (PC), the main phospholipid cellular membranes. We found Toll-like receptor (TLR) activation enhances uptake macrophages through induction transporter CTL1. rapidly phosphorylated mobilized Kennedy pathway leading increased production. Inhibition or phosphorylation attenuated NLRP3...
ABSTRACT Vaginal dryness is a common symptom associated with vulvovaginal atrophy of menopause. The impact vaginal very significant as it negatively affects quality life, daily activities, sexual satisfaction well on interpersonal relationships. Symptoms often underreported and undertreated. Recently, lubricants moisturizers have been applied one the alternative safe approaches to relieve for women mild moderate dryness. We evaluated safety beneficial effects new type estrogen-free gel,...
ABSTRACT Leishmania spp. are obligate intracellular parasites that must be internalized by phagocytic cells to evade immune responses and cause disease. The uptake of both promastigotes (insect-stage parasites) amastigotes (proliferative stage in humans mice) phagocytes is thought mainly host cell-driven, not parasite-driven. Our previous work indicates Src Abl family kinases facilitate entry into macrophages pathogenesis murine cutaneous leishmaniasis. Here we demonstrate spleen tyrosine...
SUMMARY Macrophages contribute to the induction and resolution of inflammation play a central role in chronic low-grade cardiovascular diseases caused by atherosclerosis. Human milk oligosaccharides (HMOs) are complex unconjugated glycans unique human that benefit infant health act as innate immune modulators. Here, we identify HMO 3’sialyllactose (3’SL) natural inhibitor TLR4-induced macrophages endothelium. Transcriptome analysis revealed 3’SL attenuates selected set inflammatory gene...