A5036740985- Photochromic and Fluorescence Chemistry
- Angiogenesis and VEGF in Cancer
- bioluminescence and chemiluminescence research
- Click Chemistry and Applications
- Molecular Sensors and Ion Detection
- Photoreceptor and optogenetics research
- Biosensors and Analytical Detection
- Nonlinear Optical Materials Studies
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- Chemical Synthesis and Analysis
- Ubiquitin and proteasome pathways
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Advanced Fluorescence Microscopy Techniques
- Monoclonal and Polyclonal Antibodies Research
- Nanoplatforms for cancer theranostics
- Glycosylation and Glycoproteins Research
- Chemical Reactions and Mechanisms
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Proteomics Techniques and Applications
- Reproductive System and Pregnancy
- Marine Sponges and Natural Products
- COVID-19 diagnosis using AI
- Supramolecular Chemistry and Complexes
Université Paris Cité
2013-2024
Inserm
2011-2024
Unité de Technologies Chimiques et Biologiques pour la Santé
2024
Centre National de la Recherche Scientifique
2010-2024
Chimie Organique, Médicinale et Extractive et Toxicologie Expérimentale
2020
Canadian Nautical Research Society
2020
Délégation Ile-de-France Villejuif
2020
Sorbonne Paris Cité
2013-2019
Délégation Paris 5
2013-2019
PremUP
2018
Angiogenesis is tightly regulated through the binding of vascular endothelial growth factors (VEGFs) to their receptors (VEGFRs). In this context, we showed that human VEGFR1 domain 2 crystallizes in presence Zn2+, Co2+ or Cu2+ as a dimer forms via metal-ion interactions and interlocked hydrophobic surfaces. SAXS, NMR size exclusion chromatography analyses confirm formation solution Co2+, Cd2+ Cu2+. Since metal-induced dimerization masks VEGFs surface, investigated ability metal ions...
The small and synthetically easily accessible 7-diethylamino-4-thiocoumarinylmethyl photolabile protecting group has been validated for uncaging with blue light. It exhibits a significant action cross-section in the 470–500 nm wavelength range low light absorption between 350 400 nm. These attractive features have implemented living zebrafish embryos to perform chromatic orthogonal photoactivation of two biologically active species controlling biological development UV blue-cyan sources,...
In a three-dimensional (3D) representation, each protein molecule displays specific pattern of chemical and topological features, which are altered during its misfolding aggregation pathway. Generating recognizable fingerprint from such features could provide an enticing approach not only to identify these biomolecules but also gain clues regarding their folding state the occurrence pathologically lethal misfolded aggregates. We report here universal strategy generate fluorescent by...
This paper evaluates the o-hydroxycinnamic platform for designing efficient caging groups with fluorescence reporting upon one- and two-photon excitation. The model cinnamates are easily prepared in one step by coupling commercial or readily available synthons. They exhibit a large one-photon absorption that can be tuned near-UV range. Uncaging after excitation was investigated 1H NMR, UV−vis absorption, steady-state emission. In whole series, caged substrate is quantitatively released...
An optical syringe for targeted delivery of a substrate in vivo has been developed on the basis 3,5-dibromo-2,4-dihydroxycinnamic caging group. Two-photon excitation caged compound uncages (ethanol scheme) and forms coumarin whose fluorescence allows concentration released to be quantified. Single-cell release can achieved with focused laser (shown schematically zebrafish embryo).
Abstract DNA methylation is involved in the regulation of gene expression and plays an important role normal developmental processes diseases, such as cancer. methyltransferases are enzymes responsible for on position 5 cytidine a CpG context. In order to identify characterize novel inhibitors these enzymes, we developed fluorescence‐based throughput screening by using short duplex immobilized 96‐well plates. We have screened 114 flavones flavanones inhibition murine catalytic Dnmt3a/3L...
We have implemented a noninvasive optical method for the fast control of protein activity in live zebrafish embryo. It relies on releasing fused to modified estrogen receptor ligand binding domain from its complex with cytoplasmic chaperones, upon local photoactivation nonendogenous caged inducer. Molecular dynamics simulations were used design cyclofen-OH, photochemically stable inducer specific 4-hydroxy-tamoxifen (ER(T2)). Cyclofen-OH was easily synthesized two steps good yields. At...
We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells live zebrafish embryo. Optical uncaging caged precursor nonendogeneous steroid by one- or two-photon illumination was used to restore activity CreERT2 fusion protein specific target cells. This labels irreversibly inducing recombination an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution gene expression. technique could be more generally investigate...
A new generation of surface-enhanced Raman scattering encoded-nanoparticles has been designed by combining aryl diazonium salt chemistry and gold nanoparticles.
This paper evaluates a series of photolabile protecting groups with built-in fluorescence reporting. They rely on readily available o-acetoxyphenyl methyloxazolones as activated precursors. Alcohol substrates are easily caged. The resulting photoactivable esters exhibit large one- and two-photon uncaging cross sections. alcohol quantitatively released in 1:1 molar ratio strongly fluorescent coumarin coproduct that serves reporter to quantify substrate delivery.
Nuclear staining by cationic triphenylamines is spontaneous in fixed cells while a cytoplasm–nucleus translocation strictly dependent on light illumination living and concomitant to fast cell death process.
Cells at disease onset are often associated with subtle changes in the expression level of a single or few molecular components, making traditionally used biomarker-driven clinical diagnosis challenging task. We demonstrate here design DNA nanosensor array multichannel output that identifies normal pathological state cell based on alteration its global proteomic signature. Fluorophore-encoded single-stranded (ssDNA) strands were coupled via supramolecular interaction surface-functionalized...
Diabetes is a major global health concern, with millions of annual deaths. Monitoring glucose levels vital for clinical management, and urine samples offer noninvasive alternative to blood samples. Optical techniques sensing have gained notable traction due their cost-effectiveness portability. Among these methods, surface-enhanced Raman spectroscopy (SERS) has attracted considerable attention thanks its remarkable sensitivity multiplexing capabilities. However, challenges remain in...
Preeclampsia is a hypertensive pregnancy disease associated with massive increase in sFlt-1 (soluble form of the vascular endothelial growth factor 1) maternal circulation, responsible for angiogenic imbalance and dysfunction. Pilot studies suggest that extracorporeal apheresis may reduce circulating prolong pregnancy. Nonspecific systems have potential adverse effects because capture many other molecules. Our concept based on specific competitive approach using VEGF (vascular factor)...
The design, synthesis, conformational studies and binding affinity for VEGF receptors of a collection linear cyclic peptide analogues the N-terminal α-helix fragments 13-25 1-13 Vammin are described. Linear 13(14)-mer peptides were designed with help an AGADIR algorithm prepared following solid-phase synthetic protocols. Cyclic derivatives on-resin from precursors conveniently located Glu Lys residues, by formation amide linkages. Conformational analysis, CD NMR, showed that most synthesized...
With a case-control cohort of COVID samples, this study proposes methodology to use hypothesis-free sensor array strategy for classifying clinical samples. The protocol includes the design experiments, data extraction, treatment and analysis.
Cancer angiogenesis is mainly initiated by vascular endothelial growth factors (VEGFs). On the basis of reported crystal structures three natural ligands (VEGF-A, -B, and PlGF) with major receptors VEGFR-1 VEGFR-2, we scanned receptor-binding epitopes these designing linear cyclic peptides aim to disrupt VEGF-A/VEGFR-1 interaction, which implicated in cancer development. The ability inhibit this interaction was evaluated an ELISA-based assay. Several peptides, especially those mimicking loop...
Previously designed cyclic peptide antagonist c[YYDEGLEE]-NH2 disrupts the interaction between vascular endothelial growth factor (VEGF) and its receptors (VEGFRs). It represents a promising tool in fight against cancer age-related macular degeneration. We described this paper optimization of lead by C-terminal modification. A new strategy for synthesis peptides is developed, improving cyclisation efficiency. At 100 µM, several with an aromatic group flexibly linked at end showed...
The v114* cyclic peptide has been identified as a tight vascular endothelial growth factor (VEGF) ligand. Here we report on the use of isothermal titration calorimetry (ITC), 96-well plate competition assay, and circular dichroism (CD) to explore binding determinants new set related peptides. Anti-VEGF antibodies are currently used in clinic for regulating angiogenesis cancer age-related macular degeneration treatment. In this context, our aim is develop smaller molecular entities with high...
Eine optische Spritze für den Transport eines Substrats in vivo beruht auf der lichtaktivierbaren 3,5-Dibrom-2,4-dihydroxyzimtsäure-Gruppe. Zweiphotonenanregung setzt das Substrat (hier Ethanol) frei; dabei entsteht ein fluoreszierendes Cumarinderivat, mit dessen Hilfe die Konzentration an freigesetztem ermittelt werden kann. Mit einem fokussierten Laser gelingt gezielte Freisetzung einzelnen Zellen (schematisch Zebrafischembryo gezeigt).
Protein-protein interactions (PPIs) have emerged as important targets for pharmaceutical intervention because of their essential role in numerous physiological and pathological processes, but screening efforts using small-molecules led to very low hit rates. Linear peptides could represent a quick effective approach discover initial PPI hits, particularly if they inherent ability adopt specific peptide secondary structures. Here, we address this hypothesis through linear helical library,...
The use of functionalized magnetic particles is increasing because they simplify the analytical process and yield promising results in a wide range applications. Particularly, streptavidin-coated beads offer possibility rapid very efficient grafting biomolecules. Unfortunately, current methods to monitor compute this are cumbersome scarce. We describe herein simple, rapid, reliable chemiluminescent assay we have developed check rate beads. power also relies on its ability predict amount...
Proteins play a crucial role in determining disease states humans, making them prime targets for the development of diagnostic sensors. The developed sensor array is used to investigate global proteomic changes by fingerprinting multifactorial model urine simulating phenylketonuria and serum from preeclamptic pregnant women. Here, we report fluorescence-based chemical sensing that exploits host–guest interaction between cucurbit[7]uril (CB[7]) fluorescent triphenylamine derivatives (TPA)...