A5056742939- Retinal Diseases and Treatments
- Circadian rhythm and melatonin
- Angiogenesis and VEGF in Cancer
- Retinal Imaging and Analysis
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Advanced Glycation End Products research
- Glaucoma and retinal disorders
- Dietary Effects on Health
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinopathy of Prematurity Studies
- Atherosclerosis and Cardiovascular Diseases
- Apelin-related biomedical research
- Connective Tissue Growth Factor Research
- Mesenchymal stem cell research
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Diabetes Treatment and Management
- Immune cells in cancer
- MicroRNA in disease regulation
- Retinal and Optic Conditions
- Diet and metabolism studies
- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Acute Myeloid Leukemia Research
Indiana University – Purdue University Indianapolis
2016-2025
Indiana University School of Medicine
2016-2025
Indiana University
2016-2025
Neurosciences Institute
2025
University School
2020-2025
Hypertension Institute
2015-2024
Midwest Eye Institute
2022
Indiana University Indianapolis
2015-2020
University of Florida
2008-2015
Royal Victoria Hospital
2009
Intermittent fasting (IF) protects against the development of metabolic diseases and cancer, but whether it can prevent diabetic microvascular complications is not known. In
The present epidemic of diabetes is resulting in a worldwide increase cardiovascular and microvascular complications including retinopathy. Current thinking has focused on local influences the retina as being responsible for development this diabetic complication. However, contribution circulating cells maintenance, repair, dysfunction vasculature now becoming appreciated. Diabetic individuals have fewer endothelial progenitor (EPCs) their circulation these diminished migratory potential,...
We tested the hypothesis that activation of protective arm renin angiotensin system, angiotensin-converting enzyme 2 (ACE2)/angiotensin-(1-7) [Ang-(1-7)]/Mas receptor axis, corrects vasoreparative dysfunction typically seen in CD34+ cells isolated from diabetic individuals. Peripheral blood patients with diabetes were compared those nondiabetic controls. Ang-(1-7) restored impaired migration and nitric oxide bioavailability/cGMP response to stromal cell–derived factor resulted a decrease...
Endothelial progenitor cells (EPCs), critical for mediating vascular repair, are dysfunctional in a hyperglycemic and/or hypercholesterolemic environment. Their dysfunction contributes to the progression of diabetic macro- and microvascular complications. Activation "cholesterol-sensing" nuclear receptors, liver X receptors (LXRα/LXRβ), protects against atherosclerosis by transcriptional regulation genes important promoting cholesterol efflux inhibiting inflammation. We hypothesized that LXR...
In this study, we assessed whether Per2 clock gene-mutant mice exhibit a vascular phenotype similar to diabetes. (B6.129-Per2(tm1Drw)/J) or wild-type control 4 and 12 months of age were used. To evaluate diabetes-like in mutant mice, retina was quantified for mRNA expression, degree diabetic retinopathy evaluated. Bone marrow neuropathy studied by staining femurs tyrosine hydroxylase (TH) neurofilament 200 (NF-200). The rate proliferation quantification bone progenitor cells (BMPCs)...
By using pseudorabies virus expressing green fluorescence protein, we found that efferent bone marrow-neural connections trace to sympathetic centers of the central nervous system in normal mice. However, this was markedly reduced type 1 diabetes, suggesting a significant loss marrow innervation. This innervation associated with change hematopoiesis toward generation more monocytes and an altered diurnal release rodents patients diabetes. In hypothalamus granular insular cortex mice...
Electroacupuncture (EA) performed in rats and humans using limb acupuncture sites, LI-4 LI-11, GV-14 GV-20 (humans) Bai-hui (rats) increased functional connectivity between the anterior hypothalamus amygdala mobilized mesenchymal stem cells (MSCs) into systemic circulation. In human subjects, source of MSC was found to be primarily adipose tissue, whereas rodents tissue sources were considered more heterogeneous. Pharmacological disinhibition rat enhanced sympathetic nervous system (SNS)...
purpose. Vascular repair by marrow-derived endothelial progenitor cells (EPCs) is impaired during diabetes, although the precise mechanism of this dysfunction remains unknown. The hypothesis for study was that progressive basement membrane (BM) modification advanced glycation end products (AGEs) contributes to impairment EPC reparative function after diabetes-related injury. methods. EPCs isolated from peripheral blood were characterized immunocytochemistry and flow cytometry. interactions...
Dysregulation of circadian rhythmicity is identified as a key factor in disease pathogenesis. Circadian controlled at both transcriptional and post-transcriptional level suggesting the role microRNA (miRNA) double-stranded RNA (dsRNA) this process. Endonuclease Dicer controls miRNA dsRNA processing, however regulation not known. Here we demonstrate robust diurnal oscillations expression central peripheral clock control systems including suprachiasmatic nucleolus (SCN), retina, liver, bone...
Purpose: Diabetic retinopathy (DR) is a leading cause of visual impairment. Müller cells in DR are dysfunctional due to downregulation the inwardly rectifying potassium channel Kir4.1. Metformin, commonly used oral antidiabetic drug, known elicit its action through 5′ adenosine monophosphate-activated protein kinase (AMPK), cellular metabolic regulator; however, effect on Kir4.1 channels unknown. For this study, we hypothesized that metformin treatment would correct circadian rhythm...
Abstract INTRODUCTION Late‐onset Alzheimer's Disease (LOAD) is the predominant form of disease (AD), and apolipoprotein E ( APOE ) ε4 a strong genetic risk factor for LOAD. As an integral part central nervous system, retina displays variety abnormalities in Our study focused on age‐dependent retinal impairments humanized APOE4 ‐knock‐in (KI) APOE3 ‐KI mice developed by Model Organism Development Evaluation Late‐Onset (MODEL‐AD) consortium. METHODS All experiments were performed 52‐ to...
Abstract The suprachiasmatic nucleus (SCN) regulates circadian rhythms and influences physiological behavioral processes. Disruptions in (CRD) are observed type 2 diabetes (T2D), importantly, CRD acts as an independent risk factor for T2D its associated complications. BMAL1, a clock gene, is vital sustaining optimal rhythm function. However, the therapeutic potential of BMAL1 overexpression SCN to rectify neurovascular deficits has yet be investigated. In this study, db/db mice,...
Alzheimer's disease (AD), particularly late-onset AD (LOAD), affects millions worldwide, with the apolipoprotein E4 (APOE4) allele being a significant genetic risk factor. Retinal abnormalities are hallmark of LOAD, and our recent study demonstrated age-related retinal impairments in APOE4-knock-in (KI) mice, highlighting that occur before onset cognitive decline these mice. Muller cells (MCs), key glia, vital for health, their dysfunction may contribute to seen AD. MCs maintain potassium...
Estimation of advanced glycation end products (AGEs) by determining fluorescence is based on the use a standard calibrator prepared incubating bovine serum albumin (BSA) and glucose at 37 degrees C for 60 days. In present study we attempted to reduce duration incubation 4 days increasing temperature 50 C. It noteworthy that resulted in rapid production an AGE-BSA within Aminoguanidine reduced intensity glycation-induced fluorescence, while addition lysine intensified reaction, as shown...
<h3>Background:</h3> Diabetic retinopathy is associated with accumulation of advanced glycation end products in the retinal microvasculature. LR-90 an effective multistage inhibitor renoprotective and anti-inflammatory properties. <h3>Aim:</h3> To explore role progression experimental diabetic retinopathy. <h3>Methods:</h3> Streptozotocin-induced Sprague–Dawley rats were treated (50 mg/l drinking water) for up to 32 weeks. At study, eyes enucleated subjected trypsin digestion staining light...
Autologous CD34+ cells are widely used for vascular repair; however, in individuals with diabetes and microvascular disease these dysfunctional. In this study, we examine expression of the clock genes Clock, Bmal, Per1, Per2, Cry1, Cry2 diabetic nondiabetic origin determine small encoding RNA (miRNA) profile cells. The degree retinopathy (DR) was assessed. As acquired mature endothelial markers, they exhibit robust oscillations genes. siRNA treatment revealed Per2 as only gene necessary to...
OBJECTIVE Peripheral blood CD34+ cells from diabetic patients demonstrate reduced vascular reparative function due to decreased proliferation and diminished migratory prowess, largely resulting nitric oxide (NO) bioavailability. The level of TGF-β, a key factor that modulates stem cell quiescence, is increased in the serum type 2 patients. We asked whether transient TGF-β1 inhibition would improve their ability. RESEARCH DESIGN AND METHODS To inhibit protein expression, were treated ex vivo...