A5058587748- 3D Printing in Biomedical Research
- Retinal Diseases and Treatments
- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Aortic aneurysm repair treatments
- Dietary Effects on Health
- Immune cells in cancer
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Additive Manufacturing and 3D Printing Technologies
- Extracellular vesicles in disease
- Retinopathy of Prematurity Studies
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Retinal Development and Disorders
- Redox biology and oxidative stress
- Nitric Oxide and Endothelin Effects
- Diabetic Foot Ulcer Assessment and Management
- Single-cell and spatial transcriptomics
- Cardiomyopathy and Myosin Studies
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiac Fibrosis and Remodeling
- Diet and metabolism studies
Indiana University – Purdue University Indianapolis
2016-2025
Indiana University School of Medicine
2016-2025
University of Indianapolis
2017-2022
Indiana University
2020-2021
The Ohio State University
2006-2020
Lung Institute
1999-2020
University School
2020
Hypertension Institute
2018
National Institute of Research and Development for Biological Sciences
1994-2018
Indiana University Indianapolis
2017
Intermittent fasting (IF) protects against the development of metabolic diseases and cancer, but whether it can prevent diabetic microvascular complications is not known. In
Abstract —We studied the association between production of reactive oxygen species, actin organization, and cellular motility. We have used an endothelial cell monolayer–wounding assay to demonstrate that cells at margin wound thus created produced significantly more free radicals than did in distant rows. The rate incorporation monomers into filaments was fastest margin, where heightened detected. tested effect decreasing species on migration polymerization. NADPH inhibitor diphenylene...
Reports demonstrate the role of M-CSF (CSF1) in tumor progression mouse models as well prognostic value macrophage numbers breast cancer patients. Recently, a subset CD14+ monocytes expressing Tie2 receptor, once thought to be predominantly expressed on endothelial cells, has been characterized. We hypothesized that increased levels CSF1 tumors can regulate differentiation Tie2- Tie2+ phenotype. treated human with and found significant increase CD14+/Tie2+ positivity. To understand if...
Abstract Adipose tissue is a rich source of multipotent mesenchymal stem-like cells, located in the perivascular niche. Based on their surface markers, these have been assigned to two main categories: CD31−/CD45−/CD34+/CD146− cells (adventitial stromal/stem [ASCs]) and CD31−/CD45−/CD34−/CD146+ (pericytes [PCs]). These populations display heterogeneity unknown significance. We hypothesized that aldehyde dehydrogenase (ALDH) activity, functional marker primitivity, could help better define ASC...
Recently a protocol is established to obtain large quantities of human induced pluripotent stem cells (iPSC)-derived endothelial progenitors, called colony forming (ECFC), and candidate smooth-muscle (SMFC). Here, the suitability for assembling in spheroids, larger 3D cell constructs tested. iPSC-derived ECFC SMFC are labeled with tdTomato eGFP, respectively. Spheroids formed ultra-low adhesive wells, their dynamic proprieties studied by time-lapse microscopy, or confocal microscopy. also...
Angiotensin-converting enzyme 2 (ACE2) is the primary of vasoprotective axis renin angiotensin system (RAS). We tested hypothesis that loss ACE2 would exacerbate diabetic retinopathy by promoting bone marrow dysfunction. ACE2-/y were crossed with Akita mice, a model type 1 diabetes. When comparing -Akita mice to we observed reduction both short-term and long-term repopulating hematopoietic stem cells, shift hematopoiesis toward myelopoiesis, an impairment lineage- c-kit+ stem/progenitor cell...
Electroacupuncture (EA) performed in rats and humans using limb acupuncture sites, LI-4 LI-11, GV-14 GV-20 (humans) Bai-hui (rats) increased functional connectivity between the anterior hypothalamus amygdala mobilized mesenchymal stem cells (MSCs) into systemic circulation. In human subjects, source of MSC was found to be primarily adipose tissue, whereas rodents tissue sources were considered more heterogeneous. Pharmacological disinhibition rat enhanced sympathetic nervous system (SNS)...
The small GTPase rac1 controls actin redistribution to membrane ruffles in fibroblasts and other cell types, as well the activation of NADPH oxidase phagocytes. We explored possibility that these two processes could be related. used a replication-deficient adenoviral vector overexpress constitutively active form rac1, racV12, human mouse aortic endothelial cells. show here that, addition ruffle formation, racV12 induced an increase total amount F-actin within Concurrently,...
Linear arrays of cells, or cell columns, have been observed in the extracellular matrix prior to neovascularization, but their nature and significance remains elusive. Based on emerging evidence implicating a role for monocytes macrophages (MC/MPH) vasculogenesis, we hypothesized that MC/MPH also can form linear branched facilitating co-migration spatial arrangement other types. To test this hypothesis, studied distribution effected by chemotactic migration novel vitro vivo models...
Doxorubicin (DOX) and its derivatives are used as chemotherapeutic drugs to treat cancer patients. However, production of DOX-mediated reactive oxygen species (ROS) by prolonged use these has been found cause dilative cardiomyopathy congestive heart failure. Thus various preventive modalities have developed avoid this side effect. We that the oxidant-induced toxicity in cardiac cells could be minimized hyperthermia-induced small heat shock protein 27 (HSP27); is, acts an endogenous...
Fc gamma receptor (Fc R) clustering by immune complexes activates multiple signaling pathways leading to phagocytosis. We and others have previously reported that Akt is phosphorylated in response R clustering. However, the functional consequence of activation not known. Using Raw 264.7 macrophage cells transfected overexpress either constitutively active myristoylated (Myr)-Akt or a dominant-negative CAAX-Akt bone marrow macrophages (BMMs) from wild-type transgenic mice expressing...
The ability to control the differentiation of adult hematopoietic stem cells (HSCs) would promote development new cell-based therapies treat multiple degenerative diseases. Systemic injection NaIO3 was used ablate retinal pigment epithelial (RPE) layer in C57Bl6 mice and initiate neural degeneration. HSCs infected ex vivo with lentiviral vector expressing RPE-specific gene RPE65 restored a functional RPE layer, typical phenotype including coexpression another marker, CRALBP, photoreceptor...
Retinopathy of prematurity (ROP) is a disorder the developing retina preterm infants. ROP can lead to blindness because abnormal angiogenesis that result suspended vascular development and vaso-obliteration leading severe retinal stress hypoxia. We tested hypothesis use human progenitor cell combination, bone marrow-derived CD34+ cells wall-derived endothelial colony-forming (ECFCs), would synergistically protect vasculature in mouse model ROP, called oxygen-induced retinopathy (OIR). alone,...
In response to elevated glucocorticoid levels, erythroid progenitors rapidly expand produce large numbers of young erythrocytes. Previous work demonstrates hematopoietic changes in rodents exposed various physical and psychological stressors, however, the effects chronic stress on erythropoiesis has not be delineated. We employed laboratory, clinical genomic analyses a murine model restraint (RST) examine influence erythropoiesis. Mice RST demonstrated markers early expansion involving...
Purpose: We investigated whether subthreshold retinal phototherapy (SRPT) was associated with recruitment of bone marrow (BM)–derived cells to the neurosensory retina (NSR) and RPE layer. Methods: GFP chimeric mice wild-type (WT) were subjected SRPT using a slit-lamp infrared laser. Duty cycles 5%, 10%, 15%, 20% (0.1 seconds, 250 mW, spot size 50 μm) 30 applications placed 100 μm from optic disc. In adoptive transfer studies, GFP+ given intravenously immediately after WT received SRPT....