A5046028979- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Pancreatitis Pathology and Treatment
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- AI in cancer detection
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- COVID-19 epidemiological studies
- Transplantation: Methods and Outcomes
- Liver Disease Diagnosis and Treatment
- Renal cell carcinoma treatment
- Mathematical Biology Tumor Growth
- Osteoarthritis Treatment and Mechanisms
- Pain Mechanisms and Treatments
- Kruppel-like factors research
- Rabbits: Nutrition, Reproduction, Health
- Long-Term Effects of COVID-19
- Testicular diseases and treatments
- Genetic Syndromes and Imprinting
- Cell Adhesion Molecules Research
- Genomic variations and chromosomal abnormalities
- Neuroendocrine Tumor Research Advances
Johns Hopkins University
2017-2025
Cancer Research Center
2019-2025
Johns Hopkins Medicine
2019-2025
University of Baltimore
2024
Medical University of South Carolina
2023-2024
Koç University
2022
Marmara University
2022
University of Alabama at Birmingham
2022
University of Michigan
2022
Vanderbilt University Medical Center
2022
Men experience more severe outcomes from coronavirus disease 2019 (COVID-19) than women. Golden Syrian hamsters were used to explore sex differences in the pathogenesis of a human clinical isolate acute respiratory syndrome 2 (SARS-CoV-2).
Intraductal papillary mucinous neoplasms (IPMNs) are non-invasive precursor lesions that can progress to invasive pancreatic cancer and classified as low-grade or high-grade based on the morphology of neoplastic epithelium. We aimed compare genetic alterations in regions same IPMN order identify molecular underlying progression.We performed multiregion whole exome sequencing tissue samples from 17 IPMNs with both dysplasia (76 regions, including 49 27 dysplasia). reconstructed phylogeny for...
Testicular germ cell tumors (TGCTs) are among the most responsive solid cancers to conventional chemotherapy. To elucidate underlying mechanisms, we developed a mouse TGCT model featuring cell-specific Kras activation and Pten inactivation. The resulting mice malignant, metastatic TGCTs composed of teratoma embryonal carcinoma, latter which exhibited stem characteristics, including expression pluripotency factor OCT4. Consistent with epidemiological data linking human testicular cancer risk...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest forms cancer. Accumulating evidence indicates tumor microenvironment highly associated with tumorigenesis through regulation cellular physiology, signaling systems, and gene expression profiles cancer cells. Yet mechanisms by which evolves from normal pancreas architecture to precursor lesions invasive poorly understood. Obtaining high-content high-resolution information a complex in large volumetric landscapes...
Abstract Osteoarthritis (OA) is one of the leading causes disability, affecting over 500 million adults worldwide. Previous studies have found that various inflammatory factors can contribute to pathogenesis OA, including complement in synovial fluid OA patients. However, this disease still not known, and only therapy severe total joint replacements. Total replacements are invasive, expensive, affect quality life. Here we show when human articular chondrocytes stimulated with...
Pancreatic ductal adenocarcinoma (PDAC) develops from 2 known precursor lesions: a majority (∼85%) pancreatic intraepithelial neoplasia (PanIN), and minority intraductal papillary mucinous neoplasms (IPMNs). Clinical classification of PanIN IPMN relies on combination low-resolution, 3-dimensional (D) imaging (computed tomography, CT), high-resolution, 2D (histology). The definitions currently rely heavily size. IPMNs are defined as macroscopic: generally >1.0 cm visible in CT, PanINs...
Partial heart transplantation is a new approach to deliver growing valve implants. transplants differ from because only the part of containing necessary transplanted. This allows partial grow, similar valves in transplants. However, transplant biology remains unexplored. critical barrier progress field. Without knowledge about specific transplantation, children with are empirically treated like known grow. In order field, an animal model for necessary. Here, we contribute our surgical...
Methods for spatially resolved cellular profiling using thinly cut sections have enabled in-depth quantitative tissue mapping to study inter-sample and intra-sample differences in normal human anatomy disease onset progression. These methods often profile extremely limited regions, which may impact the evaluation of heterogeneity due sub-sampling. Here, we applied CODA, a deep learning-based platform, reconstruct three-dimensional (3D) microanatomy grossly cancer-containing pancreas...
Pancreatic ductal adenocarcinoma is a rare but lethal cancer. Recent evidence suggests that pancreatic intraepithelial neoplasia (PanIN), microscopic precursor lesion gives rise to cancer, larger and more prevalent than previously believed. Better understanding of the growth-law dynamics PanINs may improve our ability understand how miniscule fraction makes transition invasive Here, using three-dimensional tissue mapping, we analyzed >1000 found size distributed according power law. Our...
To identify matrix metalloproteinase (MMP)-2 and -9 in CSF from dogs with intracranial tumors.CSF 55 tumors 37 control dogs.Latent active MMP-2 were identified by use of gelatin zymography. The presence MMPs the was compared that clinically normal had idiopathic or cryptogenic epilepsy peripheral vestibular disease. Relationships between MMP-9 cell counts protein also investigated.Latent found samples all dogs, although not detected any sample. Latent a subset histologically documented...
Pancreatic intraepithelial neoplasia (PanIN) is a precursor to pancreatic cancer and represents critical opportunity for interception. However, the number, size, shape, connectivity of PanINs in human tissue samples are largely unknown. In this study, we quantitatively assessed using CODA, novel machine-learning pipeline 3D image analysis that generates quantifiable models large pieces pancreas with single-cell resolution. Using cohort 38 slabs grossly normal from surgical resection...
Abstract In the ongoing coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2), more outcomes are reported in males compared with females, including hospitalizations and deaths. Animal models can provide an opportunity to mechanistically interrogate causes of sex differences pathogenesis SARS-CoV-2. Adult male female golden Syrian hamsters (8-10 weeks age) were inoculated intranasally 10 5 TCID 50 SARS-CoV-2/USA-WA1/2020 euthanized at several...
Abstract Immunotherapies are generally ineffective in pancreatic ductal adenocarcinoma (PDAC). Paradoxically, inflammation is believed to play a key role PDAC development and invasion: patients suffering from chronic pancreatitis have 13-fold increase risk of developing PDAC. cells surrounded by dense network fibrotic tissue containing immunosuppressive such as regulatory T cells, tumor-associated macrophages, cancer-associated fibroblasts. Better understanding the could lead design...
SUMMARY Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer for which few effective therapies exist. Immunotherapies specifically are ineffective in pancreatic cancer, part due to its unique stromal and immune microenvironment. intraepithelial neoplasia, or PanIN, the main precursor lesion PDAC. Recently it was discovered that PanINs remarkably abundant grossly normal pancreas, suggesting vast majority will never progress cancer. Here, through construction of 48 samples cm 3...