A5086303632- Neurological Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Renal Transplantation Outcomes and Treatments
- Cardiac Ischemia and Reperfusion
- Anesthesia and Neurotoxicity Research
- Transplantation: Methods and Outcomes
- Gastric Cancer Management and Outcomes
- Traditional Chinese Medicine Analysis
- Cytomegalovirus and herpesvirus research
- Renal Diseases and Glomerulopathies
- Neuroscience and Neuropharmacology Research
- Metastasis and carcinoma case studies
- Cancer Genomics and Diagnostics
- Polyomavirus and related diseases
- Ion Transport and Channel Regulation
- Helicobacter pylori-related gastroenterology studies
- 3D Printing in Biomedical Research
- Nanoplatforms for cancer theranostics
- Neuroendocrine Tumor Research Advances
- Intraperitoneal and Appendiceal Malignancies
- Wnt/β-catenin signaling in development and cancer
- Tissue Engineering and Regenerative Medicine
- Gastrointestinal Tumor Research and Treatment
- Chronic Kidney Disease and Diabetes
- Esophageal Cancer Research and Treatment
Zhejiang University
2008-2024
Shanghai Clinical Research Center
2022-2024
Hangzhou Center for Disease Control and Prevention
2022-2024
National Clinical Research Center for Digestive Diseases
2020-2024
Sichuan University
2024
West China Hospital of Sichuan University
2024
Zhejiang Center for Disease Control and Prevention
2020-2023
First Affiliated Hospital Zhejiang University
2011-2020
State Administration of Traditional Chinese Medicine of the People's Republic of China
2016-2020
Stanford University
2018
Fabrication of ultrasmall single‐component omnipotent nanotheranostic agents integrated with multimodal imaging and multiple therapeutic functions becomes more practically relevant but challenging. In this article, sub 10 nm Bi 2 S 3 biocompatible particles are prepared through a bovine serum albumin (BSA)‐mediated biomineralization process under ambient aqueous conditions. Owing to the size colloidal stability, resulting nanoparticles (NPs) present outstanding blood circulation behavior...
Ischemic postconditioning has been found to decrease brain infarct area and spinal cord ischemic injury. In this study, we tested the hypothesis that reduces global cerebral ischemia/reperfusion-induced structural functional injury in rats.Ten-minute ischemia was induced by 4-vessel occlusion male Sprague-Dawley rats. The animals underwent consisting of 3 cycles 15-second/15-second (Post-15/15), 30-second/30-second (Post-30/30), or 60-second/15-second (Post-60/15) reperfusion/reocclusion...
Acute renal allograft rejection (ARAR) after kidney transplantation associated with reduced graft survival and eventual failure is poorly diagnosed in hospitals. Here, we report the development of Artificial bioMarker Probes (AMPros) for sensitive urinalysis ARAR murine models. AMPros spontaneously go to kidneys systemic administration, specifically react prodromal immune biomarkers activate their near-infrared fluorescence signals cell-mediated rejection, efficiently undergo excretion into...
Abstract Podocyte injury and the appearance of proteinuria are features minimal-change disease (MCD). Cyclosporin A (CsA) tacrolimus (FK506) has been reported to reduce in patients with nephrotic syndrome, but mechanisms remain unknown. We, therefore, investigated protective CsA FK506 on a rat model MCD induced by puromycin aminonucleoside (PAN) vitro cultured mouse podocytes. Our results showed that treatment decreased via mechanism associated reduction foot-process fusion desmin, recovery...
Abstract Organ-on-a-chip systems have been increasingly recognized as attractive platforms to assess toxicity and develop new therapeutic agents. However, current organ-on-a-chip are limited by a “single pot” design, which inevitably requires holistic analysis limits parallel processing. Here, we developed digital combining microwell array with cellular microspheres, significantly increased the parallelism over traditional for drug development. Up 127 uniform liver cancer microspheres in...
Abstract miR-122 is a highly expressed liver microRNA that activated perinatally and aids in regulating cholesterol metabolism promoting terminal differentiation of hepatocytes. Disrupting expression can re-activate embryo-expressed adult-silenced genes, ultimately leading to the development hepatocellular carcinoma (HCC). Here we interrogate transcriptome at various time points after genomic excision determine cellular consequences oncogenesis. Loss leads specific progressive increases...
Abstract BACKGROUND. The incidence of lymph node metastasis is high in patients who have pT3 gastric cancer. However, the impact total retrieved nodes (tLNs) on staging and survival these not clear. METHODS. For this study, authors examined 1895 with cancer underwent surgery at Yonsei University Medical College from January 1987 to June 2000. RESULTS. Four hundred sixty (24.3%) were diagnosed pT3N0 Patients had < 31 tLNs (25th percentile) less advanced (N) stage than other ( P .001)....
Podocytes play important roles in the progression of diabetic kidney disease (DKD) and these are closely associated with cytoskeletal actin dynamics. N-Methyl-d-aspartate receptors (NMDARs), which consist two functional NR1 subunits regulatory NR2 subunits, widely expressed brain but also found podocytes. Here, we increased expression mouse models podocytes incubated high glucose (HG). In mice, knockdown using lentivirus carrying NR1-shRNA ameliorated pathological features DKD, reversed...
Abstract Background This study aimed to understand the mechanistic role of N -methyl-D-aspartate receptor (NMDAR) in acute fibrogenesis using models vivo ureter obstruction and vitro TGF-β administration. Methods Acute renal fibrosis (RF) was induced mice by unilateral ureteral (UUO). Histological changes were observed Masson’s trichrome staining. The expression levels NR1, which is functional subunit NMDAR, fibrotic epithelial-to-mesenchymal transition markers measured immunohistochemical...
Abstract Donor‐derived cell‐free DNA (dd‐cfDNA) is a promising biomarker for monitoring allograft status. However, whether dd‐cfDNA can reflect real‐time anti‐rejection treatment effects remains unclear. We prospectively recruited 28 patients with acute renal rejection, including 5 ABMR, 12 type IA or IB and 11 IIA IIB rejection. levels in peripheral blood were measured using human single nucleotide polymorphism (SNP) locus capture hybridization. The percentage of (dd‐cfDNA%) declined...
Although renal fibrosis can advance chronic kidney disease and progressively lead to end-stage failure, no effective anti-fibrotic drugs have been clinically approved. To aid drug development, we developed a biomimetic progression model on-chip evaluate effects of natural killer cell-derived extracellular vesicles pirfenidone (PFD) across different fibrotic stages. First, the dynamic interplay between fibroblasts kidney-derived matrix (ECM) resembling fibrogenic niche demonstrated that...
Long‐term exposure to peritoneal dialysate with high glucose (HG) leads fibrosis and thus decreases dialysis efficiency. In this study, we explored the role of β‐catenin in process. C57BL/6 mice received daily intraperitoneal injection 10% body weight saline (control), 4.25% fluid (PDF), or PDF combined 5 mg·kg −1 inhibitor ICG‐001 (PDF+ICG) for 30 days. Also, epithelial cells (mPECs) were cultured 10 μ m (HG+ICG) 48 h. We found greater thickness parietal peritoneum PDF‐treated mice....
Antibody-mediated rejection (ABMR) is a major cause of dysfunction and loss transplanted kidney. The current treatments for ABMR involve nonspecific inhibition clearance T/B cells or plasma cells. However, the prognosis patients following treatment poor. T follicular helper (Tfh) play an important role in allograft-specific antibodies secreting cell (PC) development. Tfh are therefore considered to be therapeutic targets antibody hypersecretion disorders, such as transplant autoimmune...
Background/Aims: Congenital heart defects (CHD) can occur with upper limbs deformities. Holt-Oram syndrome is the main type of heart-hand syndromes, characterized by limb radial ray malformations, CHD and/or conduction abnormalities. Mutations TBX5 gene, most which are found within T-box domain, one cause disease. We aimed to find disease in a family two children exhibiting symptoms while parents tend be normal. Methods: Chromosomal microarray analysis and exome sequencing were applied...
Peritoneal fibrosis is a severe complication arising from long-term peritoneal dialysis (PD). Tamoxifen (Tamo) has been clinically proven effective in series of fibrotic diseases, such as PD-associated encapsulating sclerosis (EPS), but the mechanisms underlying Tamoxifen's protective effects are yet to be defined. In present study, C57BL/6 mice received intraperitoneal injections either saline, 4.25% high glucose (HG) PD fluid (PDF) or PDF plus each day for 30 days. attenuated thickening...
Abstract Acute renal allograft rejection (ARAR) after kidney transplantation associated with reduced graft survival and eventual failure is poorly diagnosed in hospitals. Here, we report the development of A rtificial bio M arker Pro bes (AMPros) for sensitive urinalysis ARAR murine models. AMPros spontaneously go to kidneys systemic administration, specifically react prodromal immune biomarkers activate their near‐infrared fluorescence signals cell‐mediated rejection, efficiently undergo...
Allograft rejection after renal transplantation remains a challenge to overcome. Interleukin (IL)-21, cytokine with pleiotropic effects, maintains immune homeostasis post-transplantation. Here, we report higher levels of IL-21 in kidney transplant recipients non-rejection (NR) than T cell-mediated (TCMR, P < 0.001) and antibody-mediated (ABMR, = 0.005). We observed negative correlation between creatinine (Cr) (P 0.016). The receiving operating characteristic (ROC) curve showed promising...