A5085733014- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Gut microbiota and health
- Inflammatory Bowel Disease
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Reproductive Physiology in Livestock
- Microbial infections and disease research
- Dietary Effects on Health
- Reproductive System and Pregnancy
- Cancer Immunotherapy and Biomarkers
- Chemokine receptors and signaling
- Platelet Disorders and Treatments
- Mycobacterium research and diagnosis
- IL-33, ST2, and ILC Pathways
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Research and Treatments
- Autophagy in Disease and Therapy
Broad Institute
2022-2025
Brigham and Women's Hospital
2020-2025
Harvard University
2020-2025
Massachusetts General Hospital
2023-2025
Seoul National University
2015-2022
Institute for Basic Science
2016-2019
Namseoul University
2017
Cell-cell interactions in the central nervous system play important roles neurologic diseases. However, little is known about specific molecular pathways involved, and methods for their systematic identification are limited. Here, we developed a forward genetic screening platform that combines CRISPR-Cas9 perturbations, cell coculture picoliter droplets, microfluidic-based fluorescence-activated droplet sorting to identify mechanisms of cell-cell communication. We used SPEAC-seq (systematic...
The strength of the T cell receptor interaction with self-ligands affects antigen-specific immune responses. However, precise function and underlying mechanisms are unclear. Here, we demonstrate that naive CD8+ cells relatively high self-reactivity phenotypically heterogeneous owing to varied responses type I interferon, resulting in three distinct subsets, CD5loLy6C-, CD5hiLy6C-, CD5hiLy6C+ cells. differ from CD5loLy6C- CD5hiLy6C- terms gene expression profiles functional properties....
Abstract Bacteriophages, simply phages, have long been used as a potential alternative to antibiotics for livestock due their ability specifically kill enterotoxigenic Escherichia coli (ETEC), which is major cause of diarrhea in piglets. However, the control ETEC infection by phages within intestinal epithelial cells, and relationship with host immune responses, remain poorly understood. In this study, we evaluated effect phage EK99P-1 against K99-infected porcine cell line (IPEC-J2). Phage...
Abstract Continuous contact with self-major histocompatibility complex (MHC) ligands is essential for survival of naïve T cells but not memory cells. This surprising finding implies that cell subsets may vary in their relative T-cell receptor (TCR) sensitivity. Here we show CD8 + TCR sensitivity correlates inversely levels CD5, a marker strong self-MHC reactivity. We also lower than In both situations, hypo-responsiveness applies only to short-term signalling events and proliferation,...
Abstract Low-pathogenicity avian influenza H9N2 remains an endemic disease worldwide despite continuous vaccination, indicating the need for improved vaccine strategy. Bacillus subtilis ( B. ), a gram-positive and endospore-forming bacterium, is non-pathogenic species that has been used in probiotic formulations both animals humans. The objective of present study was to elucidate effect spores as adjuvants chickens administered inactivated virus H9N2. Herein, adjuvanticity demonstrated by...
Naïve CD8+ T cell quiescence is maintained at a steady state. Although this state of involves various cell-intrinsic and cell-extrinsic regulators, the mechanisms underlying regulation remain incompletely understood. Here, we found that signal transducer activator transcription 1 (STAT1), key factor downstream interferon receptor (IFNR) signaling, plays role in maintaining naïve quiescence. STAT1-deficient mice showed enhanced proliferation peripheral cells, which resulted an abnormal...
Abstract T follicular regulatory (TFR) cells limit Ab responses, but the underlying mechanisms remain largely unknown. In this study, we identify Fgl2 as a soluble TFR cell effector molecule through single-cell gene expression profiling. Highly expressed by cells, directly binds to B especially light-zone germinal center well helper (TFH) and regulates TFH in context-dependent type 2 isotype–specific manner. induces of Prdm1 panel checkpoint molecules, including PD1, TIM3, LAG3, TIGIT,...
Gut inflammation involves contributions from immune and non-immune cells, whose interactions are shaped by the spatial organization of healthy gut its remodeling during inflammation. The crosstalk between fibroblasts cells is an important axis in this process, but our understanding has been challenged incomplete cell-type definition biogeography. To address challenge, we used MERFISH to profile expression 940 genes 1.35 million imaged across onset recovery a mouse colitis model. We...
Respiratory syncytial virus (RSV) is a major pathogen that infects lower respiratory tract and causes common disease. Despite serious pathological consequences with this virus, effective treatments for controlling RSV infection remain unsolved, along poor innate immune responses induced at the initial stage of infection. Such defense mechanism against leads us to study role alveolar macrophage (AM) one primary cell types in may contribute protective As an strategy enhancing anti-viral...
Probiotics can be an effective treatment for atopic dermatitis (AD), while their mechanism of action is still unclear. Here, we induced AD in mice with 2,4-dinitrochlorobenzene and administrated YK4, a probiotic mixture consisting Lactobacillus acidophilus CBT LA1, L. plantarum LP3, Bifidobacterium breve BR3, B. lactis BL3. Then, have validated the underlying alleviation by YK4 from intestinal systematic immunological perspectives. Administration alleviated symptoms suppressing expression...
Abstract The maternal immune system tolerates semi-allogeneic placental tissues during pregnancy. Fas ligand (FASLG) and tumor necrosis factor superfamily 10 (TNFSF10) are known to be components of tolerance in humans mice. However, the role FASLG TNFSF10 process has not been studied pigs, which form a true epitheliochorial type placenta. Thus, present study examined expression function their receptors at maternal-conceptus interface pigs. endometrium conceptus expressed receptor mRNAs...
γδ T cells, known to be an important source of innate IL-17 in mice, provide critical contributions host immune responses. Development and function cells are directed by networks diverse transcription factors. Here we examine the role zinc finger factor, Kruppel-like factor 10 (KLF10), regulation IL-17–committed CD27- (γδ27--17) cells. We found selective augmentation Vγ4+ γδ27- with higher production KLF10-deficient mice. Surprisingly, CD127hi γδ27--17 expressed levels CD5 than their...
The gastrointestinal tract is the first organ directly affected by fasting. However, little known about how fasting influences intestinal immune system. Intestinal dendritic cells (DCs) capture antigens, migrate to secondary lymphoid organs, and provoke adaptive responses. We evaluated changes of DCs in mice with short-term their effects on protective immunity against Listeria monocytogenes (LM). Fasting induced an increased number CD103+CD11b- both small lamina propria (SILP) mesenteric...
Abstract Gastrointestinal tract is the first organ to be directly affected upon fasting. However, little known about how fasting influences intestinal immune system. In present study, we focused on changes of dendritic cells (DCs) in mice short-term and influence protective immunity against Listeria monocytogenes (LM) infection. We found that induces an increased number CD103 + CD11b - DCs both small lamina propria (SI LP) mesenteric lymph nodes (mLN) SI LP undergo active proliferation...
Abstract Cross-presentation of epitope from exogenous antigen onto MHC I molecule enhances CD8+ T cell activity is important in host protective immune responses against virus infections and cancer. We previously reported that polysorbitol transpoter (PST), prepared sorbitol diacrylate low-molecular-weight polyethylenimine, induces a long-term specific B response phagolysosomal escape antigens macrophages via mechanism called ‘proton-sponge effect’. Therefore, we hypothesize PST could enhance...