A5009748809- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- HIV Research and Treatment
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Extracellular vesicles in disease
- Neonatal Respiratory Health Research
- Immune Cell Function and Interaction
- Plant Reproductive Biology
- Single-cell and spatial transcriptomics
- Respiratory viral infections research
- Respiratory Support and Mechanisms
- COVID-19 Clinical Research Studies
- Radiomics and Machine Learning in Medical Imaging
- Cancer Cells and Metastasis
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS drug development and treatment
- Long-Term Effects of COVID-19
- T-cell and B-cell Immunology
- Metabolomics and Mass Spectrometry Studies
- Thermal Regulation in Medicine
- Viral-associated cancers and disorders
- Cancer Immunotherapy and Biomarkers
Lomonosov Moscow State University
2019-2024
Endocrinology Research Center
2024
Moscow State University
2023-2024
Yandex (Russia)
2021
Abstract SARS-CoV-2 can infiltrate the lower respiratory tract, resulting in severe failure and a high death rate. Normally, airway alveolar epithelium be rapidly reconstituted by multipotent stem cells after episodes of infection. Here, we analyzed published RNA-seq datasets demonstrated that four different lung epithelial cell types express entry factors, including Ace2 . Thus, potentially infected SARS-CoV-2, which may lead to defects regeneration capacity partially accounting for...
The enzymatic dissociation of human solid tissues is a critical process for disaggregating extracellular matrix and the isolation individual cells various applications, including immortalizing primary cells, creating novel cell lines, performing flow cytometry its specialized type, FACS, as well conducting scRNA-seq studies. Tissue procedures should yield intact, highly viable single that preserve morphology surface markers. However, endocrine tissues, such adrenal gland tumors, thyroid...
SUMMARY Among flowering plants, genome size varies remarkably, by >2200‐fold, and this variation depends on the loss gain of noncoding DNA sequences that form distinct heterochromatin complexes during interphase. In plants with giant genomes, most chromatin remains condensed interphase, forming a dense network threads called interphase chromonemata. Using super‐resolution light electron microscopy, we studied ultrastructure chromonemata after replication in root meristem nuclei Nigella...
An increased frequency of B-cell lymphomas is observed in human immunodeficiency virus-1 (HIV-1)-infected patients, although HIV-1 does not infect B cells. Development may be potentially due to the action Tat protein, which actively released from HIV-1-infected cells, on uninfected The exact mechanism Tat-induced lymphomagenesis has yet been precisely identified. Here, we ectopically expressed either or its TatC22G mutant devoid transactivation activity RPMI 8866 lymphoblastoid cell line and...
Lung inflammation, pneumonia, is an acute respiratory disease of varying etiology that has recently drawn much attention during the COVID-19 pandemic as lungs are among main targets for SARS-CoV-2. Multiple other etiological agents associated with pneumonias. Here, we describe a newly-recognized pathology, namely abnormal lipid depositions in patients who died from well non-COVID-19 Our analysis both semi-thin and Sudan III-stained lung specimens revealed extracellular intracellular...
Abstract SARS-CoV-2 can infiltrate the lower respiratory tract, resulting in severe failure and a high death rate. Normally, airway alveolar epithelium be rapidly reconstituted by multipotent stem cells after episodes of infection. Here, we analyzed published RNA-seq datasets demonstrated that four different lung epithelial cell types express entry factors, including Ace2 . Thus, potentially infected SARS-CoV-2, which may lead to defects regeneration capacity partially accounting for...
Abstract Among land plants, genome sizes vary remarkably by > 2,200-fold. This variation depends on the loss and gain of non-coding DNA sequences, forming different heterochromatin complexes during interphase. In plants with a giant genome, major part chromatin stays condensed in interphase, dense meshwork threads, called interphase chromonemata. Using super-resolution light electron microscopy, we studied ultrastructure chromonemata after replication root meristem nuclei Nigella...
Patients infected with human immunodeficiency virus-1 (HIV-1) have an increased incidence of B-cell lymphoma, even though HIV-1 does not infect B cells. The development lymphomas appears to be related the action transactivator protein (Tat), which is released from HIV-infected cells and penetrates uninfected cells, affecting host cell gene expression. Upon chronic infection, Tat acts on for a long time, probably allowing adapt presence viral protein. aim this work was identify study...
Eukaryotic cells are characterized by a high degree of compartmentalization their internal contents, which ensures precise and controlled regulation intracellular processes. During many processes, including different stages transcription, dynamic membrane-free compartments called biomolecular condensates formed. Transcription contain various transcription factors RNA polymerase formed low specificity interactions between protein factors, DNA nearby RNA. This review discusses recent data...
Single-cell RNA sequencing (scRNA-seq) is a revolutionary tool for studying the physiology of normal and pathologically altered tissues. This approach provides information about molecular features (gene expression, mutations, chromatin accessibility, etc.) cells, opens up possibility to analyze cell differentiation trajectories/phylogeny cell-cell interactions allows discovering new types previously unexplored processes. From clinical point view, scRNA-seq deeper more detailed analysis...