A5054477290- Marine Sponges and Natural Products
- Marine Invertebrate Physiology and Ecology
- Cellular Mechanics and Interactions
- Marine Ecology and Invasive Species
- Microtubule and mitosis dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 Clinical Research Studies
- Endoplasmic Reticulum Stress and Disease
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Antioxidant Activity and Oxidative Stress
- Molecular Biology Techniques and Applications
- Prostate Cancer Treatment and Research
- Adipose Tissue and Metabolism
- Polysaccharides and Plant Cell Walls
- Silk-based biomaterials and applications
- Toxin Mechanisms and Immunotoxins
- Autophagy in Disease and Therapy
- COVID-19 epidemiological studies
- Wound Healing and Treatments
- Neurological Disorders and Treatments
- Marine Biology and Environmental Chemistry
- SARS-CoV-2 and COVID-19 Research
- Planarian Biology and Electrostimulation
Lomonosov Moscow State University
2018-2024
Engelhardt Institute of Molecular Biology
2022-2024
Moscow State University
2023-2024
City Clinical Hospital
2021-2023
Nazarbayev University
2023
All-Russian Scientific Research Institute of Food Biotechnology
2019-2022
Federal Scientific Center for Animal Husbandry named after Academician L.K. Ernst
2020
Lactate is now considered an additional fuel or signaling molecule in the brain. In this study, using oxygen-glucose deprivation (OGD) model, we found that treatment with lactate inhibited global increase intracellular calcium ion concentration ([Ca
During the ongoing coronavirus disease 2019 (COVID-19) pandemic, many individuals were infected with and have cleared virus, developing virus-specific antibodies effector/memory T cells. An important unanswered question is what levels of T-cell antibody responses are sufficient to protect from infection.
Abstract Epithelial cells undergoing EMT experience significant alterations at transcriptional and morphological levels. However, changes in the cytoskeleton, especially cytoskeleton dynamics are poorly described. Addressing question we induced three cell lines (MCF-7, HaCaT A-549) analyzed cytoskeletal there using immunostaining life imaging of transfected with microtubule focal adhesion markers. In all studied lines, area after increased, MCF-7 A-549 became elongated, while kept aspect...
Abstract Tissues of multicellular animals are maintained due to a tight balance between cell proliferation and programmed death. Sponges early branching metazoans essential understanding the key mechanisms tissue homeostasis. This article is dedicated comparative analysis apoptosis in intact tissues two sponges, Halisarca dujardinii (class Demospongiae) Leucosolenia variabilis Calcarea). Labeled nucleotides EdU anti‐phosphorylated histone 3 antibodies reveal considerable number cycling cells...
In almost all metazoans examined to this respect, the axial patterning system based on canonical Wnt (cWnt) signaling operates throughout course of development. most metazoans, gastrulation is polar, and embryos develop morphological landmarks polarity, such as blastopore under control/regulation from cWnt signaling. However, in many cnidarian species, morphologically apolar. The question remains whether сWnt providing establishment a body axis controls morphogenetic processes involved...
Microtubule (MT) inhibitors show anti-cancer activity in a wide range of tumors vitro and demonstrate high clinical efficacy. To date they are routinely included into many chemotherapeutic regimens. While the mechanisms MT inhibitors' interactions with tubulin have been well-established, relationship between their concentration effect on neoplastic cells is not completely understood. The common notion that tumor most vulnerable during division all block them mitosis induce mitotic...
CD133 is an extensively studied marker of the most malignant tumor cell population, designated as cancer stem cells (CSCs). However, function this glycoprotein and its involvement in regulatory cascades are still poorly understood. Here we show a positive correlation between level plasma membrane expression proliferative activity Caco-2, HT-29, HUH7 lines. Despite substantial difference activities populations with different levels expression, transcriptomic proteomic profiling revealed only...
Background Prostate cancer (PC) diagnostics and treatment often present a challenging task due to subtype heterogeneity differential disease progression in patient subgroups. Hence, the critical issue is finding reliable sensitive diagnostic prognostic PC marker, especially for cases of biopsies with low percentages cells. Isoform A myosin 1C was shown be expressed cells responsible their invasive properties, however, its feasibility purposes remains elucidated. Methods To verify role...
Early diagnosis of prostate cancer is a challenging issue due to the lack specific markers. Therefore, sensitive diagnostic marker that expressed or upregulated exclusively in cells would facilitate procedures and ensure better outcome. We evaluated expression myosin 1C isoform A 5 cell lines, 41 cases, 11 benign hyperplasias. analyzed 12 surface molecules on by flow cytometry whether high low could be attributed distinct phenotype cells. Median samples lines was 2 orders magnitude higher...
Summary Rapid spread of COVID-19 pandemic made a substantial share the world population immunised by SARS-CoV-2 antigens. Infection induces development virus-specific antibodies and T cells. Ample evidence on antibody-mediated protection is contrasted elusive role cells in preventing infection. To explore impact to quantify protective levels immune responses we conducted large prospective study: 5,340 Moscow residents were evaluated for antibody cellular monitored up 300 days. The tightly...
An increased frequency of B-cell lymphomas is observed in human immunodeficiency virus-1 (HIV-1)-infected patients, although HIV-1 does not infect B cells. Development may be potentially due to the action Tat protein, which actively released from HIV-1-infected cells, on uninfected The exact mechanism Tat-induced lymphomagenesis has yet been precisely identified. Here, we ectopically expressed either or its TatC22G mutant devoid transactivation activity RPMI 8866 lymphoblastoid cell line and...
ABSTRACT When cells with a mesenchymal type of motility come into contact an adhesive substrate they adhere and start spreading by the formation lamellipodia. Using label-free approach virtual synchronization we analyzed in fibroblasts cancer cells. In all cell lines is non-linear process undergoing isotropic or anisotropic modes first fast (5–20 min) then slow (30–120 phases. 10 min area increases 2–4 times, while absolute rate initial decreases 2–8 times. Fast depends on actin...
Lung inflammation, pneumonia, is an acute respiratory disease of varying etiology that has recently drawn much attention during the COVID-19 pandemic as lungs are among main targets for SARS-CoV-2. Multiple other etiological agents associated with pneumonias. Here, we describe a newly-recognized pathology, namely abnormal lipid depositions in patients who died from well non-COVID-19 Our analysis both semi-thin and Sudan III-stained lung specimens revealed extracellular intracellular...
Diterpenoid plant hormone gibberellic acid (GA) plays an important role in regulation of growth and development is commonly used agriculture for activation food production. It known that many plant-derived compounds have miscellaneous biological effects on animals humans, influencing specific cellular functions metabolic pathways. However, the effect GA animal human cells remains controversial. We investigated cultured cell lines epidermoid origin-immortalized non-tumorigenic keratinocytes...
The development of drugs for the treatment acute kidney injury (AKI) that could suppress excessive inflammatory response in damaged kidneys is an important clinical challenge. Recently, synaptamide (N-docosahexaenoylethanolamine) has been shown to exert anti-inflammatory and neurogenic properties. aim this study was investigate effect ischemic AKI. For purpose, we analyzed expression mediators infiltration different leukocyte populations into after injury, evaluated putative receptor...
Focal adhesions (FAs) are mechanosensory structures that can convert physical stimuli into chemical signals guiding cell migration. There is a postulated correlation between FA features and motility parameters for individual migrating cells. However, which properties essential the movement of epithelial cells within monolayer remains poorly elucidated. We used real-time visualization to describe relationship migration immortalized keratinocytes (HaCaT) lung carcinoma (A549) under inhibition...