A5090425159- Digital Holography and Microscopy
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced X-ray Imaging Techniques
- Optical measurement and interference techniques
- Advanced Fluorescence Microscopy Techniques
- Image Processing Techniques and Applications
- Iron Metabolism and Disorders
- Spectroscopy and Chemometric Analyses
- Trace Elements in Health
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Electrospun Nanofibers in Biomedical Applications
- Advanced Optical Imaging Technologies
- Circadian rhythm and melatonin
- Neuroinflammation and Neurodegeneration Mechanisms
- Tissue Engineering and Regenerative Medicine
- Bee Products Chemical Analysis
- Sleep and Wakefulness Research
- 3D Printing in Biomedical Research
- Advanced Electron Microscopy Techniques and Applications
- Optical Coherence Tomography Applications
- Dental Implant Techniques and Outcomes
- Microfluidic and Bio-sensing Technologies
- Near-Field Optical Microscopy
- Erythrocyte Function and Pathophysiology
University at Albany, State University of New York
2016-2025
State University of New York
2019-2023
Albany State University
2018-2022
National Cancer Center of Georgia
2020
University of Michigan
2010-2016
Ann Arbor Center for Independent Living
2016
Michigan United
2011-2013
University of South Florida
2005-2008
We present a phase-imaging technique to quantitatively study the three-dimensional structure of cells. The method, based on simultaneous dual-wavelength digital holography, allows for higher axial range at which unambiguous phase imaging can be performed. is capable nanometer resolution. noise level, increases as result using two wavelengths, then reduced level single wavelength. method compares favorably software unwrapping, does not produce non-existent steps. Curvature mismatch between...
Most animal species operate according to a 24-h period set by the suprachiasmatic nucleus (SCN) of hypothalamus. The rhythmic activity SCN modulates hippocampal-dependent memory, but molecular and cellular mechanisms that account for this effect remain largely unknown. Here, we identify cell-type-specific structural functional changes occur with circadian rhythmicity in neurons astrocytes hippocampal area CA1. Pyramidal change surface expression NMDA receptors. Astrocytes their proximity...
ABSTRACT Osteoblasts, which orchestrate the deposition of small apatite crystals through expression nucleating proteins, have been shown to also express clock genes associated with circadian signaling pathway. We hypothesized that protein-mediated bone mineralization may be linked oscillator mechanisms functioning in peripheral tissue. In this study, Per1 ex vivo neonatal murine calvaria organ cultures was monitored for 6 days using a Per1-luciferase transgene as bioluminescent indicator...
Quantitative phase imaging (QPI) of live cells allows label-free assessment cell morphology and quantitative estimation thickness, therefore volume. Of the techniques available for QPI, digital holographic microscopy (DHM) is an accurate reliable high-resolution technique, but assembly operation a DHM capable setup can require significant optical engineering experience specialized equipment that may not be practical many labs. Transport intensity equation (TIE) QPI method compatible with...
We present a study of the three-dimensional structure cancer cells using dual-wavelength phase-imaging digital holographic microscopy. Phase imaging objects with optical height variation greater than wavelength light is ambiguous and causes phase wrapping. By comparing two images recorded at different wavelengths, can be accurately unwrapped. The unwrapping method computationally fast straightforward, it process complex topologies. Additionally, limitations on total are significantly...
We present a simultaneous dual-wavelength phase-imaging digital holographic technique demonstrated on porous coal samples. The use of two wavelengths enables us to increase the axial range at which unambiguous phase imaging can be performed, but also increases noise. employ noise reduction "fine map" algorithm, uses two-wavelength map as guide correct single-wavelength image. Then, resulting fine is reduced level A comparison software unwrapping presented. simple way correcting curvature...
A noninvasive quality monitoring of tissue-engineered constructs is a required component any successful tissue-engineering technique. During 2-week production period, ex vivo produced oral mucosa-equivalent (EVPOMEs) may encounter adverse culturing conditions that might compromise their and render them ineffective. We demonstrate the application near-infrared Raman spectroscopy to in vitro EVPOMEs during manufacturing process, with ultimate goal applying this technology situ monitor grafted...
Planar solid supported single lipid bilayers on mica, glass, or other inorganic surfaces have been widely used as models for cell membranes. To more closely mimic the membrane environment, soft hydrophilic polymer cushions were introduced between hard substrate and bilayer to completely avoid possible substrate-lipid interactions. In this article, sum frequency generation (SFG) vibrational spectroscopy was examine compare assembled CaF(2) prism surface poly (L-lactic acid) (PLLA) cushion. By...
Coherent anti-Stokes Raman scattering (CARS) microscopy can be used as a powerful imaging technique to identify chemical compositions of complex samples in biology, biophysics, medicine, and materials science. In this work we developed CARS microscopic system capable hyperspectral imaging. By employing an ultrafast laser source, photonic crystal fiber, scanning microscope together with spectral detection by highly sensitive back-illuminated cooled charge-coupled device (CCD) camera, were...
Cytosolic lipids participate in the growth, development, and overall health of mammalian oocytes including many roles cellular homeostasis.
Core/shell nanofibers are becoming increasingly popular for applications in tissue engineering.Nanofibers alone provide surface topography and increased area that promote cellular attachment; however, core/shell the versatility of incorporating two materials with different properties into one.Such synthetic can mechanical degradation required to make a construct mimics vivo tissue.Many variations these fibers be produced.The challenge lies ability characterize quantify post fabrication.We...
We describe a microscopic setup implementing phase imaging by digital holographic microscopy (DHM) and transport of intensity equation (TIE) methods, which allows the results both measurements to be quantitatively compared for either live cell or static samples. Digital is well-established method that provides robust reconstructions, but requires sophisticated interferometric system. TIE, on other hand, directly compatible with bright-field microscopy, more susceptible noise artifacts....
We combined sum-frequency generation (SFG) vibrational spectroscopy with coherent anti-Stokes Raman scattering (CARS) in one system to examine both surface and bulk structures of materials the same geometry without need move sample. Poly(methyl methacrylate) (PMMA) polystyrene (PS) thin films were tested before after plasma treatment. The sensitivities SFG CARS by varying polymer film thickness using a lipid monolayer.
In maxillofacial and oral surgery, there is a need for the development of tissue-engineered constructs. They are used reconstructions due to trauma, dental implants, congenital defects, or cancer. A noninvasive monitoring fabrication constructs at production implantation stages done in real time extremely important predicting success grafts. We demonstrated Raman spectroscopic probe system, its design application, real-time ex vivo produced mucosa equivalent (EVPOME) monitoring. performed...
We used phase microscopy and Raman spectroscopic measurements to assess the response of in vitro rat C6 glial cells following methamphetamine treatment real time. Digital holographic (DHM) three-dimensional (3-D) tomographic nanoscopy allow live cell cultures, which yield information about volume changes. Tomographic imaging provides 3-D refractive index distribution associated with morphology biological samples. DHM similar information, but for a larger population cells. Morphological...
Transferrin (Tf) is an essential serum protein which delivers iron throughout the body via transferrin-receptor (TfR)-mediated uptake and release in early endosomes. Currently, there no robust method to assay population of iron-bound Tf intact cells tissues. Raman hyperspectral imaging detected spectral peaks that correlated with tumor xenografts sections (~1270-1300 cm
Engineering salivary glands is of interest due to the damaging effects radiation therapy and autoimmune disease Sjögren's syndrome on gland function. One current problems in tissue engineering that vitro studies often fail predict vivo regeneration failure cells interact with scaffolds single cell types are typically used for these studies. Although poly (lactic co glycolic acid) (PLGA) nanofiber have been growth epithelial cells, PLGA has low compliance do not penetrate scaffolds. Using a...
Three-dimensional digital holographic microscopic phase imaging of objects that are thicker than the wavelength light is ambiguous and results in wrapping. In recent years, several unwrapping methods employed two or more wavelengths were introduced. These compare information obtained from each extend range unambiguous height measurements. A straightforward dual-wavelength method presented which allows for a flexible tradeoff between maximum sample amount noise can tolerate. For highly...