A5101428122- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Nitric Oxide and Endothelin Effects
- Memory and Neural Mechanisms
- Pharmacological Receptor Mechanisms and Effects
- Ion channel regulation and function
- Tryptophan and brain disorders
- Neuroendocrine regulation and behavior
- Treatment of Major Depression
- Electron Spin Resonance Studies
- Nicotinic Acetylcholine Receptors Study
- Genetics and Neurodevelopmental Disorders
- Helicobacter pylori-related gastroenterology studies
- Cellular transport and secretion
- Stress Responses and Cortisol
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical Analysis and Sensing Techniques
- Neurogenesis and neuroplasticity mechanisms
- Gastric Cancer Management and Outcomes
- Gastrointestinal Tumor Research and Treatment
- Cardiac electrophysiology and arrhythmias
- Hemophilia Treatment and Research
- Mathematical Biology Tumor Growth
Toyohashi Municipal Hospital
2013-2024
Tokyo Metropolitan Institute of Medical Science
2001-2019
Yokohama City University
2016
Tokyo Institute of Psychiatry
2002-2011
Nagoya University
2008
Nagoya University Hospital
2008
Nagoya City University Hospital
2008
National Institutes of Health
1993-1997
National Institute of Neurological Disorders and Stroke
1992-1997
Oita University Hospital
1996
Serotonin (5-HT)-induced changes in the levels of intracellular Ca++ were analyzed human platelets, using Ca+(+)-sensitive dye 1-(2-(5'-carboxyoxazol-2'-yl)-6-aminobenzofuran-5-oxy)-2-(2' -amino-5'- methylphenox)-ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester, to investigate regulation 5-HT2 receptor function. mobilized a dose-dependent fashion from basal level 98 +/- 2.7 and up 211 5.8 nM with an EC50 value for 5-HT 0.2 microM. Ketanserin, antagonist, reversed (10...
Abstract Diverse protocadherins (Pcdhs), which are encoded as a large cluster (composed of α, β and γ clusters) in the genome, localized to axons synapses. The Pcdhs have been proposed contribute generation sophisticated neural networks regulate brain function. To address molecular roles regulating individual behavior, here we generated knockdown mice Pcdh‐α proteins examined their behavioral abnormalities. There two alternative splicing variants constant region, A B isoforms, with different...
3,4-Methylendioxymethamphetamine (MDMA) has both stimulatory and hallucinogenic properties which make its psychoactive effects unique different from those of typical psychostimulant agents. The present study investigated the MDMA on extracellular dopamine (DAex) serotonin (5-HTex) levels in striatum prefrontal cortex (PFC) using vivo microdialysis techniques mice lacking DA transporters (DAT) and/or 5-HT (SERT). Subcutaneous injection (3, 10 mg/kg) significantly increased striatal DAex...
Phencyclidine (PCP), a noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist, increases locomotor activity in rodents and causes schizophrenia-like symptoms humans. Although activation of the dopamine (DA) pathway is hypothesized to mediate these effects PCP, precise mechanisms by which PCP induces its remain be elucidated. The present study investigated effect on extracellular levels DA (DAex) striatum prefrontal cortex (PFC) using vivo microdialysis mice lacking NMDA channel ε1 or...
N-methyl-D-aspartate (NMDA) receptor plays important roles in learning and memory. NMDA receptors are a tetramer that consists of two glycine-binding subunits GluN1, glutamate-binding (i.e., GluN2A, GluN2B, GluN2C, GluN2D), combination GluN2 subunit GluN3 GluN3A or GluN3B), subunits. Recent studies revealed the specific expression distribution each deeply involved neural excitability, plasticity, synaptic deficits. The present article summarizes reports on dysfunction responsible various...
Noncompetitive N-methyl-d-aspartate (NMDA) receptor antagonists evoke a behavioral and neurobiological syndrome in experimental animals. We previously reported that phencyclidine (PCP), an NMDA antagonist, increased locomotor activity wildtype (WT) mice but not GluN2D subunit knockout mice. Thus, the aim of present study was to determine whether is involved PCP-induced motor impairment. PCP or UBP141 (a antagonist) induced potent impairment WT KO By contrast, CIQ, GluN2C/2D potentiator,...
To expand on the nature of regional cerebral vulnerability to ischemia, release dopamine (DA) and dopaminergic (D1 D2) receptors were investigated in Mongolian gerbils subjected bilateral carotid artery occlusion (15 min) alone or with reflow (1-2 h). Extracellular cortical striatal content DA its metabolites was measured by microdialysis using HPLC electrochemical detection. The kinetic properties D1 and/or D2 receptor binding sites determined membranes use radiolabeled ligands...
Abstract: The monoamine transporters are the main targets of psychostimulant drugs, including methamphetamine (METH) and cocaine. Interestingly, rewarding effects cocaine retained in dopamine transporter (DAT) knockout (KO) mice, while serotonin (SERT) DAT double KO mice do not exhibit conditioned place preference (CPP) to These data suggest that SERT inhibition decreases psychostimulants. To further test this hypothesis, present study, we investigated intraperitoneal (i.p.) injections 20...
Monoamine transporters are the main targets of methamphetamine (METH). Recently, we showed that fluoxetine, a selective serotonin reuptake inhibitor (SSRI), decreased METH conditioned place preference (CPP), suggesting transporter (SERT) inhibition reduces rewarding effects METH. To further test this hypothesis, in present study investigated additional SSRIs, paroxetine and fluvoxamine, on CPP C57BL/6J mice. In test, pretreatment with 20 mg/kg abolished for METH, whereas 100 fluvoxamine...
Abstract: The effect of endothelins (ET‐1 and ET‐3) on 86 Rb + uptake as a measure K was investigated in cultured rat brain capillary endothelium. ET‐1 or ET‐3 dose‐dependently enhanced (EC 50 = 0.60 ± 0.15 21.5 4.1 n M , respectively), which inhibited by the selective ET A receptor antagonist BQ 123 (cyclo‐ d ‐Trp‐ ‐Asp‐Pro‐ ‐Val‐Leu). Neither B agonists IRL 1620 [ N ‐succinyl‐(Glu 9 ,‐Ala 11,15 )‐ET‐1] sarafotoxin S6c, nor 1038 [(Cys 11 ,Cys 15 had any uptake. Ouabain (inhibitor Na ,K...
Objectives CXBK mice, recombinant inbred mice derived from C57BL/6By and BALB/cBy progenitors, display reduced morphine-induced analgesia. Earlier we reported that expressed a amount of the major transcript, MOR-1 mRNA, mu-opioid receptor gene. The mRNA contains normal coding region an abnormally long untranslated region. Methods results To identify nucleotide-sequence difference between first characterized 3′ which was largely unknown. A rapid amplification cDNA ends-PCR analysis revealed...
Genetic factors are hypothesized to be involved in interindividual differences opioid sensitivity. Inbred mouse strains that genetically different and isogenic within each strain useful for elucidating the genetic mechanisms underlying opioid-induced analgesia.We examined effects of morphine 10 inbred strains, including wild-derived have a wide range diversity, BLG2, CHD, KJR, MSM, NJL, PGN2, SWN. We also performed full sequencing 5' flanking region exons mu receptor gene Oprm1 analyzed...