A5027083465- Preterm Birth and Chorioamnionitis
- Pregnancy and preeclampsia studies
- Neonatal Respiratory Health Research
- Reproductive System and Pregnancy
- Reproductive tract infections research
- Advanced Proteomics Techniques and Applications
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Neuropeptides and Animal Physiology
- Cancer, Hypoxia, and Metabolism
- Receptor Mechanisms and Signaling
- Antimicrobial Peptides and Activities
- Mass Spectrometry Techniques and Applications
- Gene expression and cancer classification
- Cervical Cancer and HPV Research
- Prenatal Screening and Diagnostics
- Infant Nutrition and Health
- Platelet Disorders and Treatments
- Chronic Kidney Disease and Diabetes
- Enzyme-mediated dye degradation
- Advanced Biosensing Techniques and Applications
- Fibroblast Growth Factor Research
- Atrial Fibrillation Management and Outcomes
- Neuroendocrine Tumor Research Advances
- Heparin-Induced Thrombocytopenia and Thrombosis
The University of Texas Southwestern Medical Center
2018
Southwestern Medical Center
2018
Thomas Jefferson University
2009-2016
Oregon Health & Science University
2004-2015
University of Washington
2006-2013
Nizam's Institute of Medical Sciences
2009-2013
St Michaels Hospital
2013
University of Eastern Finland
2013
Kuopio University Hospital
2013
American Diabetes Association
2013
The identification of biomarkers to noninvasively detect prediabetes/diabetes will facilitate interventions designed prevent or delay progression frank diabetes and its attendant complications. purpose this study was characterize the human salivary proteome in type-2 identify potential diabetes. Whole saliva from control diabetic individuals characterized by multidimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS). Label-free quantification used differentially abundant...
The mac25 cDNA was originally cloned from leptomeningial cells and subsequently reisolated through differential display as a sequence preferentially expressed in senescent human mammary epithelial cells. deduced amino acid of the propeptide shares 20-25% identity to insulin-like growth factor-binding proteins (IGFBPs), suggesting that could be another member IGFBP family.In present study, we have generated recombinant (rh-mac25) baculovirus expression system assessed its affinity for IGFs...
The insulin-like growth factor (IGF) binding proteins (IGFBPs) modulate the actions of factors in endocrine, paracrine, and autocrine settings. Additionally, some IGFBPs appear to exhibit biological effects that are IGF independent. six high-affinity have been characterized date 40–60% amino acid sequence identity overall, with most conserved sequences their NH 2 COOH termini. We recently demonstrated product mac25/IGFBP-7 gene, which shows significant conservation terminus, including an...
We have employed recently developed blind modification search techniques to generate the most comprehensive map of post-translational modifications (PTMs) in human lens constructed date. Three aged lenses, two which had moderate cataract, and one young control were analyzed using multidimensional liquid chromatography mass spectrometry. In total, 491 sites proteins identified. There 155 vivo PTM crystallins: 77 previously reported 78 newly detected sites. Several these undetected by...
OBJECTIVE—Diabetic nephropathy is a serious complication of both type 1 and 2 diabetes, and, unless arrested, leads to end-stage renal disease. Current diagnosis consists urine assays microalbuminuria, which have inadequate specificity sensitivity. RESEARCH DESIGN AND METHODS—We used proteomic analyses identify novel biomarkers in from diabetic patients with demonstrated normo-, micro-, or macroalbuminuria. Samples were analyzed by fluorescence two-dimensional (2-D) differential in-gel...
With the increasing availability of de novo sequencing algorithms for interpreting high-mass accuracy tandem mass spectrometry (MS/MS) data, there is a growing need programs that accurately identify proteins from results. De sequences derived spectra peptides often contain ambiguous regions where exact amino acid order cannot be determined. One problem this poses sequence alignment difficulty in distinguishing discrepancies due to errors actual genomic variation and posttranslational...
Bombesin is a tetradecapeptide originally isolated from frog skin and demonstrated to have wide range of actions in mammals. Based on structural homology similar biological activities, gastrin-releasing peptide (GRP) has been considered the mammalian equivalent bombesin. We previously reported that frogs both GRP bombesin, which therefore are distinct peptides. now report cloning bombesin receptor subtype (BB4) higher affinity for than GRP. PCR was used amplify cDNAs related known receptors...
Algorithms that can robustly identify post-translational protein modifications from mass spectrometry data are needed for data-mining and furthering biological interpretations. In this study, we determined a mass-based alignment algorithm (OpenSea) de novo sequencing results could post-translationally modified peptides in high-throughput environment. A complex digest of proteins human cataractous lens, tissue containing high abundance proteins, was analyzed using two-dimensional liquid...
Spontaneous preterm birth (SPTB) is a major contributor to perinatal morbidity and mortality. However, the diagnosis of labor (PTL) that leads difficult, there pressing need for improved diagnosis. We utilized multidimensional liquid chromatography-tandem mass spectrometry (LC/LC−MS/MS; MudPIT) Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) identify potential biomarkers PTL SPTB. MudPIT analysis identified 205 proteins in cervical−vaginal fluid (CVF), 28 which...
Cervical-vaginal fluid (CVF) is a potential rich source of biomarkers for enhancing our understanding human parturition and pathologic conditions affecting pregnancy. In this study, we performed comprehensive survey the CVF proteome in pregnancy utilizing multidimensional liquid chromatography (2D-LC) coupled with mass spectrometry gel-electrophoresis-based protein separation identification. total, 150 unique proteins were identified using multiple identification algorithms. Metabolism (32%)...
Amniotic fluid (AF) is a significant contributor to fetal health and constitutes potential rich source of biomarkers for diagnosis maternal disorders. In this study, we performed comprehensive survey the proteins expressed in AF, combining gel liquid-based fractionation approaches coupled with LC−MS/MS analysis. Two-dimensional Liquid Chromatography (2D-LC) analysis identified 118 nonredundant high confidence. One- two-dimensional electrophoresis in-gel digestion 101 proteins. Combining both...
Down syndrome (DS) is the most prevalent chromosomal disorder, accounting for significant morbidity and mortality. Definitive diagnosis requires invasive amniocentesis, current maternal serum-based testing a false-positive rate of about 5% to detect 85% affected pregnancies. We have performed comprehensive proteomic analysis identify potential serum biomarkers DS. First- second-trimester samples DS gestational age-matched controls were analyzed using multiple, complementary approaches,...
In Brief OBJECTIVE: To evaluate the potential clinical utility of serum biomarkers for first-trimester prediction gestational diabetes mellitus (GDM). METHODS: Maternal concentrations glycosylated (Sambucus nigra lectin–reactive) fibronectin, adiponectin, sex hormone–binding globulin, placental lactogen, and high-sensitivity C-reactive protein (CRP) were measured at 5–13 weeks gestation in a case-control study 90 pregnant women with subsequent development GDM 92 control group participants....
Intra-amniotic infection (IAI) is associated with preterm birth and perinatal mortality. To identify potential biomarkers, we performed a comprehensive survey of the cervical−vaginal fluid (CVF) proteome from primate IAI model utilizing multidimensional protein identification technology (LC/LC−MS/MS) MALDI-TOF−MS analyses. Analyses CVF identified 205 unique proteins differential expression 27 in controls samples. Protein signatures immunodetection specific biomarkers can be employed for...
We systematically characterized maternal serum proteome in women with clinical preeclampsia (PE) and asymptomatic early pregnancy that subsequently developed PE. Clinical PE cohort comprised 30 patients mild PE, severe 58 normotensive women. Preclinical included 149 whose samples were collected at 8-14 gestational weeks whom later 40 Serum was analyzed enzyme-linked immunosorbent assays used for protein quantification. In fibronectin, pappalysin-2, choriogonadotropin-beta, apolipoprotein...
Preeclampsia is a major pregnancy complication that results in significant maternal and infant mortality, most of which occurs low middle-income countries. The accurate timely diagnosis preeclampsia critical management affected pregnancies to reduce fetal/neonatal morbidity yet difficulties remain establishing the rigorous based on clinical parameters alone. Biomarkers detect biochemical disease have been proposed as complements or alternatives criteria improve diagnostic accuracy. This...
Journal Article Progesterone receptor, but not estradiol messenger ribonucleic acid is expressed in luteinizing granulosa cells and the corpus luteum rhesus monkeys Get access Y A Chandrasekher, Chandrasekher 1Department of Physiology, Oregon Health Sciences University, Portland 97201. Search for other works by this author on: Oxford Academic Google Scholar M H Melner, Melner S R Nagalla, Nagalla L Stouffer Endocrinology, Volume 135, Issue 1, 1 July 1994, Pages 307–314,...
Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoenzyme produced by cellulose-degrading cultures of the wood-degrading basidiomycete Phanerochaete chrysosporium. CDH contains one flavin adenine dinucleotide (FAD) and heme b per molecule, it oxidizes cellobiose to cellobionolactone. In this report, a 2.4-kb cDNA encoding was isolated screening expression library P. chrysosporium OGC101 with CDH-specific polyclonal antibody. The encodes 755-amino-acid protein predicted mass 80,115...