A5019107214- FOXO transcription factor regulation
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Single-cell and spatial transcriptomics
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Cancer, Lipids, and Metabolism
- Multiple Myeloma Research and Treatments
- Neurobiology and Insect Physiology Research
- Cancer, Stress, Anesthesia, and Immune Response
- Toxoplasma gondii Research Studies
- Cardiomyopathy and Myosin Studies
- Muscle Physiology and Disorders
- Protein Degradation and Inhibitors
- Tryptophan and brain disorders
- Hereditary Neurological Disorders
- Endoplasmic Reticulum Stress and Disease
- Neurological diseases and metabolism
- RNA Research and Splicing
- Autoimmune Bullous Skin Diseases
- Genetics and Physical Performance
- Cellular transport and secretion
University of Illinois Chicago
2016-2025
Ashland (United States)
2016-2021
Consejo Nacional de Investigaciones Científicas y Técnicas
2005-2012
Instituto Tecnológico de Chascomús
2005-2012
National University of General San Martín
2005-2012
Fundación Instituto Leloir
2011
The function of Retinoblastoma tumor suppressor (pRB) is greatly influenced by the cellular context, therefore consequences pRB inactivation are cell-type-specific. Here we employ single cell RNA-sequencing (scRNA-seq) to profile impact an Rbf mutation during Drosophila eye development. First, build a catalogue 11,500 wild type disc cells containing major known types. We find transcriptional switch occurring in differentiating photoreceptors at time axonogenesis. Next, map landscape mutant...
The transcription factor Forkhead box M1 (FoxM1) is overexpressed in breast cancers and correlates with poor prognosis. Mechanistically, FoxM1 associates CBP to activate Rb repress transcription. Although the activating function of cancer has been well documented, significance its repressive activity poorly understood. Using CRISPR–Cas9 engineering, we generated a mouse model that expresses FoxM1-harboring point mutations block binding while retaining ability bind CBP. Unlike FoxM1-null...
Long-term exposure to stressful situations can affect the immune system. The T-cell response is an important component of anti-tumoral immunity. Hence, impairment function induced by a chronic stressor has been postulated alter immunosurveillance tumors, thus leading worse neoplastic prognosis. Here, we show that restraint stress affects mediated immunity in mice. This was evidenced decrease mitogen-induced proliferation, reduction CD4(+)T lymphocyte number and tumor necrosis factor-alpha...
Abstract The E2F transcription factor is a key cell cycle regulator. However, the inactivation of entire family in Drosophila permissive throughout most animal development until pupation when lethality occurs. Here we show that function adult skeletal muscle essential for viability since providing muscles rescues whole-body E2F-deficient animals. Muscle-specific loss results significant reduction mass and thinner myofibrils. We demonstrate dispensable proliferation progenitor cells, but...
The importance of the retinoblastoma tumor suppressor protein pRB in cell cycle control is well established. However, less known about its role differentiation during animal development. Here, we investigated Rbf, Drosophila homolog, adult skeletal muscles. We found that depletion Rbf severely reduced muscle growth and altered myofibrillogenesis but only minimally affected myoblast proliferation. identified an Rbf-dependent transcriptional program late development distinct from canonical...
Lipid metabolism and the serine, one-carbon, glycine (SOG) methionine pathways are independently significantly correlated with estrogen receptor–negative breast cancer (ERneg BC). Here, we propose a link between lipid ERneg BC through phosphoglycerate dehydrogenase (PHGDH), rate-limiting enzyme in de novo serine pathway. We demonstrate that of paradigmatic medium-chain fatty acid octanoic leads to metabolic shift toward SOG pathways. PHGDH plays role both forward direction, contributing...
Abstract Introduction: A lipid metabolism gene signature is enriched in breast tissue at risk for estrogen receptor negative (ERneg) cancer (BC). Fatty acid (FA) exposure alters histone methylation, expression and increases metabolic flux through serine, one-carbon, glycine (SOG) methionine pathways. We hypothesize that FA induces a shift towards the SOG, increasing S-adenosylmethionine (SAM), altering expression, promoting ERneg BC. Methods: Proteomics, metabolomics, Reactive Oxygen Species...
Article19 August 2020Open Access Source DataTransparent process A cell atlas of adult muscle precursors uncovers early events in fibre-type divergence Drosophila Maria Paula Zappia Department Biochemistry and Molecular Genetics, University Illinois at Chicago, IL, USA Search for more papers by this author Lucia de Castro Majd M Ariss orcid.org/0000-0001-8013-5172 Holly Jefferson orcid.org/0000-0002-7989-2317 Abul BMMK Islam orcid.org/0000-0002-7274-0855 Genetic Engineering Biotechnology,...
Introduction Inflammatory epidermolysis bullosa acquisita (EBA) is characterized by a neutrophilic response to anti-type VII collagen (COL7) antibodies resulting in the development of skin inflammation and blistering. The antibody transfer model EBA closely mirrors this phenotype. Methods To better understand changes induced neutrophils upon recruitment from peripheral blood into lesional EBA, we performed single-cell RNA-sequencing whole dissociate capture minimally perturbed characterize...
We had previously shown that the transmembrane glycoprotein M6a, a member of proteolipid protein (PLP) family, regulates neurite/filopodium outgrowth, hence, M6a might be involved in neuronal remodeling and differentiation. In this work we focused on M6, only PLP family present Drosophila, ortholog to M6a. Unexpectedly, found decreased expression M6 leads female sterility. is expressed membrane follicular epithelium ovarioles throughout oogenesis. Phenotypes triggered by downregulation...
Abstract A lipid metabolism gene signature is associated with the risk of estrogen negative breast cancer (ER-BC). In vitro, exposure alters histone methylation affecting expression and increasing flux through various metabolic reactions; but little known about mechanism(s) linking lipids epigenetic reprogramming genesis ER-BC. Here we show that medium-chain fatty acid Octanoic Acid (OA) in preference to glucose glutamine results a shift toward serine pathway production SAM, glutathione,...
Members of the proteolipid protein family, including four-transmembrane glycoprotein M6a, are involved in neuronal plasticity mammals. Results from our group previously demonstrated that M6, only expressed Drosophila, localizes to cell membrane follicle cells. M6 loss triggers female sterility, which suggests a role for follicular remodeling. These results were basis present study, focused on function and requirements fly nervous system. The study identified two novel, tissue-regulated...
The E2F transcription factors play a critical role in controlling cell fate. In Drosophila, the inactivation of either muscle or fat body results lethality, suggesting an essential function for these tissues. However, cellular and organismal consequences inactivating tissues are not fully understood. Here, we show that loss exerts both tissue-intrinsic systemic effects. proteomic profiling E2F-deficient revealed regulates carbohydrate metabolism, conclusion further supported by metabolomic...
Summary In Drosophila , the wing disc-associated adult muscle precursors (AMPs) give rise to fibrillar indirect flight muscles (IFM) and tubular direct (DFM). To understand early transcriptional events underlying this diversification, we performed single cell RNA-sequencing experiments built a atlas of AMPs associated with third instar larval disc. Our analysis identified distinct signatures for IFM DFM that underlie molecular basis their divergence. The further revealed various states...
The canonical role of the transcription factor E2F is to control expression cell cycle genes by binding sites in their promoters. However, list putative target extensive and includes many metabolic genes, yet significance controlling these remains largely unknown. Here, we used CRISPR/Cas9 technology introduce point mutations upstream five endogenous Drosophila melanogaster . We found that impact on both recruitment varied, with glycolytic gene, Phosphoglycerate kinase ( Pgk) , being mostly...
Robust genetic systems to control the expression of transgenes in a spatial and temporal manner are valuable asset for researchers. The GeneSwitch system induced by drug RU486 has gained widespread use Drosophila community. However, some concerns were raised as negative effects seen depending on stock, transgene, stage, tissue under study. Here, we characterized adverse triggered activating adult muscles using MHC-GS-GAL4 driver. When control, mock UAS-RNAi transgene was feeding flies with...
Abstract Introduction. A lipid metabolism gene signature is associated with the risk of estrogen negative breast cancer (ER-BC). In vitro, exposure alters histone methylation affecting expression and increases flux through various metabolic reactions including those involved in serine, one-carbon, glycine (SOG) methionine. We hypothesized that lipids preference to glucose glutamine results a shift toward serine pathway increasing S-adenosylmethionine (SAM) leading changes expression....
Abstract Introduction. Oncogenic factors that are local/in-breast of great interest as they may be more specifically targetable for breast cancer prevention than systemic factors. We have identified a lipid metabolism gene signature is enriched in tissue at risk estrogen negative (ER- BC). Utilizing the medium chain fatty acid Octanoic (OA) to probe non-transformed epithelial cells, we observed increased flux through several metabolic reactions and altered histone methylation with consequent...