Abstract Aberrantly expressed miRNAs play a crucial role in the development of multiple cancer types, including chordoma. However, detailed molecular mechanisms are unclear and need to be elucidated. In this study, were screened by miRNA array analysis then confirmed real-time PCR analysis. We found that miR-16-5p was significantly downregulated chordoma, overexpression suppressed chordoma cell proliferation, invasion migration vitro vivo correlated with upregulated expression E-cadherin...

Abstract Background Recent studies indicated that exosomal programmed death-ligand 1 (PD-L1) derived from cancers could induce immunosuppression and tumor pathogenesis. However, it is unclear how exosomes influence osteosarcoma (OS) progression whether PD-L1 also exists in serum (Sr-exosomes) of patients with osteosarcoma. We examined 70 OS patients, 9 benign tumors 22 healthy donors. OS-derived were functionally evaluated vivo vitro. Results The characteristics patient cell lines confirmed...

Abstract Background Osteosarcoma (OS) is the most common primary malignant bone tumor in young people. Tumor-associated macrophages (TAMs) have been reported to play an important role development of osteosarcoma. However, detailed molecular mechanisms remain largely unknown and need be elucidated. Recently, exosomes as crucial mediator between cells microenvironment. And a lot lncRNAs act either oncogenes or suppressors In this research, we aim explore macrophages-derived exosomal lncRNA...

The transcription factor glioma-associated oncogene 1 (Gli1) has been recognized as a very important nuclear executor at the distal end of Hedgehog (Hh) signal pathway, which crucial roles in regulating many developmental processes, such pattern formation, differentiation, proliferation, and apoptosis. Overexpression patched protein Gli1 or constitutively active Indian (IHh)-parathyroid hormone-related pathway may lead to musculoskeletal tumorigenesis. However, for chondrosarcoma few studies...

Expression of COP9 signalosome subunit 3 (COPS3), an oncogene overexpressed in osteosarcoma, has been demonstrated to be significantly correlated with tumor metastasis. However, the underlying mechanism by which COPS3 promotes metastasis osteosarcoma and its role autophagy remain unknown. The expression was detected primary tissues matching lung immunohistochemistry (IHC). effect on cells investigated transwell, wound healing assays animal studies. Indicated proteins analyzed western...

<i>Background/Aim:</i> Laparoscopy-assisted surgery has proved useful in the treatment of early gastric cancer, but its use advanced cancer rarely been studied. To investigate efficacy and advantages laparoscopy-assisted distal gastrectomy (LADG) with D2 dissection lymph nodes versus conventional open (ODG) cancer. <i>Methods:</i> From January 2007 to June 2008, clinical data 66 cases LADG for were compared that 69 patients who, during same period, underwent a radical...

The use of liquid biopsy in cancer research has grown exponentially, offering potential for early detection, treatment stratification, and monitoring residual disease recurrence. Exosomes, released by cells, contain tumor-derived materials are stable biofluids, making them valuable biomarkers clinical evaluation. Bibliometric on osteosarcoma (OS) exosome-derived diagnostic is scarce. Therefore, we aimed to conduct a bibliometric evaluation studies OS biomarkers. Using the Web Science Core...

Currently, plenty of studies have demonstrated that lncRNAs can act as crucial roles during the progression various tumors, including osteosarcoma (OS), and emerging evidences indicated are abundant stable in exosomes. The objective this study is to reveal dysregulated OS plasma exosomes explore their functions OS.Microarray was performed analyze exosomal lncRNAs. Western blot, qRT-PCR assays, Dual-luciferase reporter assay were used verify interaction among cancer susceptibility 15...

Bone morphogenetic protein receptor 2 (BMPR2) has been identified in several types of cancer. However, its role osteosarcoma is largely unknown. We systematically investigated the BMPR2 cell lines, human tissue samples and xenograft models. The relationship between expression patients' survival was by bioinformatics clinical data. Wound healing assay transwell were used to detect changes migration invasion ability after transfection. In addition, downstream phosphoproteins analyzed...

The survival rate of osteosarcoma, the most prevalent primary bone tumor, has not been effectively improved in last 30 years. Hence, new treatments and drugs are urgently needed. Antiangiogenic therapy immunotherapy have good antitumor effects many kinds tumors. It is hypothesized that there may be a synergistic effect between immune checkpoint inhibitors antiangiogenic therapy. Nevertheless, its potential mechanism still unclear. Vascular endothelial growth factor receptor-2 (VEGFR2)...

Osteosarcoma is the most common primary malignant bone tumour predominantly occurring in children and adolescents with a high tendency of local invasion early metastases. Currently, immune microenvironment (TME) becoming focus studying tumours.. However, no sound evidence shows specific molecular target osteosarcoma. We downloaded gene expression profile clinical data osteosarcoma from TARGET portal, extracted normalized via R software. Then, cell infiltration assessed by CIBERSORT ESTIMATE...

Because of the modest response rate after surgery and chemotherapy, treatment osteosarcoma (OS) remains challenging due to tumor recurrence metastasis. miR-135a has been reported act as an anticarcinogenic regulator several cancers. However, its expression function in remain largely unknown. Here, we that abridged OS cells tissues, is inversely correlated with BMI1 KLF4, which are described oncogenes Ectopic inhibited cell invasion KLF4 cells. In vivo investigation confirmed acts a...

Abstract Objective: Bone morphogenetic protein receptor 2 (BMPR2) and hypoxia-inducible factor 1-α (HIF1-α) existed abnormal expression in several types of cancer. However, their expressions related roles osteosarcoma are largely unknown. Methods: To investigate the clinical significance BMPR2 HIF1-α osteosarcoma, we analyzed levels 103 specimens by immunochemistry. Meanwhile, conducted a follow-up to examine metastatic behavior overall survival (OS) patients. Results: Among tissues, 61...

It has been demonstrated that Gli1 is expressed in chondrosarcoma but not the normal articular cartilage tissues. Downregulating by small interfering RNA inhibited cells growth. Arsenic trioxide (ATO) to suppress human cancer cell growth targeting Gli1. The aim of this study was investigate effect ATO on antineoplastic capability cells. We found dose-dependent and time-dependent manners via MTT colony formation assays. In addition, treatment induced apoptosis promoted G2/M phase arrest...

F-box proteins are essential components of the Skp-cullin-F-box complex (a type E3 ubiquitin ligase), and participate in cell cycle immune responses through proteasome system. protein 39 (FBXO39) belongs to family, which has been reported be associated with cancer oncogenesis progression. The present study aimed investigate role FBXO39 osteosarcoma (OS) proliferation apoptosis vitro. It was demonstrated that U-2OS cells exhibited high expression compared HOS SaOS-2 cells. Thus, knockdown...

A patient with a history of dysphagia for 3 months was diagnosed as upper esophageal cancer by gastroscopy. After sufficient preoperative preparation, the underwent esophagectomy McKeown procedure Robotic-assisted approach. The five-port method used in both thoracic and abdominal part. jejunostomy tube placed enteral nutrition. At last, cervical esophagogastric anastomosis performed. No complications occurred during 6-day postoperative hospitalization. TNM stage T3N0M0, IIA.

Esophageal cancer is the eighth most commonly diagnosed worldwide and sixth common cause of cancer-related death. For esophageal patients without contraindications, esophagectomy remains vital remedy despite great achievement adjuvant therapy neoadjuvant therapy. However, involves two or three compartment dissection, leading to high morbidity mortality as well profound decrease in quality life. Since Dr. Franz Torek successfully conducted first transthoracic 1913, various surgical approaches...

Objective To investigate the value of a needle loop retractor in appendectomy.Methods The clinical data 50 patients with appendicitis who were admitted to Chongqing Ninth People's Hospital from January 2011 June 2012 retrospectively analyzed.Two-port laparoscopic appendectomy was performed all patients,and its efficacy analyzed.Patients followed up at out-patient department or via telephone till December 2012.Results operation successfully completed on patients.One patient received...