A5100384628- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Medical Imaging and Pathology Studies
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Lung Cancer Diagnosis and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lung Cancer Research Studies
- RNA Research and Splicing
- Cancer Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Advanced Proteomics Techniques and Applications
- Tracheal and airway disorders
- Colorectal Cancer Treatments and Studies
- Inorganic Fluorides and Related Compounds
- Neonatal Respiratory Health Research
- Pancreatic and Hepatic Oncology Research
- Metabolomics and Mass Spectrometry Studies
- Cancer, Lipids, and Metabolism
- Parathyroid Disorders and Treatments
- Cancer Mechanisms and Therapy
- Atomic and Subatomic Physics Research
- Single-cell and spatial transcriptomics
- Enhanced Recovery After Surgery
Peking University People's Hospital
2009-2024
Peking University
2009-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Guangzhou Experimental Station
2024
Fourth Hospital of Hebei Medical University
2024
Hebei Medical University
2024
Second Affiliated Hospital of Zhejiang University
2024
Jiangsu Cancer Hospital
2018-2023
Nanjing Medical University
2023
Jiangsu Province Hospital
2023
Abstract Circular RNAs (circRNA) are a class of covalently closed single-stranded that have been implicated in cancer progression. Here we identify circNDUFB2 to be downregulated non-small cell lung (NSCLC) tissues, and negatively correlate with NSCLC malignant features. Elevated inhibits growth metastasis cells. Mechanistically, functions as scaffold enhance the interaction between TRIM25 IGF2BPs, positive regulator tumor progression metastasis. This TRIM25/circNDUFB2/IGF2BPs ternary...
Endometrial cancer is the most common malignancy of lower female reproductive tract. The tumor suppressor FOXO1 downregulated in endometrial compared with normal endometrium but underlying mechanisms are not well understood. Using microRNA (miR) target prediction algorithms, we identified several miRs that potentially bind 3'-untranslated region transcripts. Expression profiling and malignant samples by quantitative real-time PCR Northern blot analysis revealed an inverse correlation between...
The role of circular RNA in cancer is emerging. A newly reported HIPK3 (circHIPK3) critical cell proliferation various types, although its non-small lung (NSCLC), has yet to be elucidated. Our results provided evidence that silencing circHIPK3 significantly impaired proliferation, migration, invasion and induced macroautophagy/autophagy. Mechanistically, we uncovered autophagy was upon loss via the MIR124-3p-STAT3-PRKAA/AMPKa axis STK11 mutant lines (A549 H838). STAT3 abrogation as well...
Somatic copy number variations (CNV) may drive cancer progression through both coding and noncoding transcripts. However, transcripts resulting from CNV are largely unknown, especially for circular RNAs. By integrating bioinformatics analyses of alerted circRNAs focal in lung adenocarcinoma, we identify a proto-oncogenic RNA (circPRKCI) the 3q26.2 amplicon, one most frequent genomic aberrations multiple cancers. circPRKCI was overexpressed adenocarcinoma tissues, part due to amplification...
To assess the benefits of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors as neoadjuvant/adjuvant therapies in locally advanced EGFR mutation-positive non-small-cell lung cancer.This was a multicenter (17 centers China), open-label, phase II, randomized controlled trial erlotinib versus gemcitabine plus cisplatin (GC chemotherapy) therapy patients with stage IIIA-N2 cancer mutations exon 19 or 21 (EMERGING). Patients received 150 mg/d (neoadjuvant therapy, 42 days;...
Abstract Deep understanding of the genomic and immunological differences between Chinese Western lung cancer patients is great importance for target therapy selection development patients. Here we report an extensive molecular immune profiling study 245 with non-small cell cancer. Tumor-infiltrating lymphocyte estimated using signatures found to be significantly higher in adenocarcinoma (ADC, 72.5%) compared squamous carcinoma (SQCC, 54.4%). The correlation alterations reveals that low...
Lung adenocarcinoma manifesting as radiological subsolid nodules has distinct cell composition within its tumor microenvironment.
Lung cancer is the leading cause of mortality, and early detection key to improving survival. However, there are no reliable blood-based tests currently available for early-stage lung diagnosis. Here, we performed single-cell RNA sequencing different cancers found that lipid metabolism was broadly dysregulated in cell types, with glycerophospholipid as most altered metabolism–related pathway. Untargeted lipidomics carried out an exploratory cohort 311 participants. Through support vector...
Abstract Studies have demonstrated that noncoding RNAs play important roles in various types of cancer; however, derived from regions genomic alterations rarely been explored, especially for circular (circRNA). Previously, we found several circRNAs were upregulated lung adenocarcinoma (LUAD) tumor tissues by RNA sequencing. Here, characterized a novel circRNA, circXPO1, LUAD, which is well-established cancer therapeutic target, XPO1. formed back-splicing exon 3 and 4 XPO1 gene. circXPO1 was...
Abstract Background Recent studies indicated that exosomal programmed death-ligand 1 (PD-L1) derived from cancers could induce immunosuppression and tumor pathogenesis. However, it is unclear how exosomes influence osteosarcoma (OS) progression whether PD-L1 also exists in serum (Sr-exosomes) of patients with osteosarcoma. We examined 70 OS patients, 9 benign tumors 22 healthy donors. OS-derived were functionally evaluated vivo vitro. Results The characteristics patient cell lines confirmed...
Background Lung adenocarcinomas (LUADs) that display radiologically as subsolid nodules (SSNs) exhibit more indolent biological behaviour than solid LUADs. SSNs, commonly encompassing pre-invasive and invasive yet early-stage adenocarcinomas, can be categorised pure ground-glass part-solid nodules. The genomic characteristics of SSNs remain poorly understood. Methods We subjected 154 SSN samples from 120 treatment-naïve Chinese patients to whole-exome sequencing. Clinical parameters...
Noncoding RNA play important roles in various biological processes and diseases, including cancer. The expression profile of circular (circRNA) has not been systematically investigated lung adenocarcinoma (LUAD). In this study, we performed genomewide transcriptome profiling coding genes, long noncoding (lncRNA), circRNA paired LUAD nontumor tissues by ribosomal RNA-depleted sequencing. detected reads were first mapped to the human genome analyze genes lncRNA, while unmapped subjected a...
Overactivation of β-adrenergic receptor (β-AR) can improve cardiac function temporarily but promotes the development and mortality heart failure (HF) in long run.CircRNA, a member noncoding RNAs, tolerate digestion exonuclease be chronic stimulator to cell.But relationship circRNA with HF remains puzzle needs explored.Here, we found that circ-HIPK3 affected concentration Ca 2+ cytoplasm by miR-17-3p through ADCY6 (Adenylate cyclase type 6).The increase caused was ameliorated overexpression...
Lung cancer had been leading mounts of deaths worldwide. Advances in genes microarray helped human further understand and identify novel circular RNAs. This study aimed at investigating the biological functions molecular mechanisms hsa_circ_0046264 lung which may be helpful early diagnosis clinical treatment. Gene data screened differential gene its downstream were found by bioinformatics analysis verified luciferase reporter assay. QRT-PCR Western blot was used to detect RNA protein levels...
Objectives: Tumor pathology examination especially epidermal growth factor receptor (EGFR) mutations molecular testing has been integral part of lung cancer clinical practices. However, the EGFR spatial distribution characteristics remains poorly investigated, which is critical to tumor heterogeneity analysis and precision diagnosis. Here, we conducted an exploratory study for label-free diagnosis mapping mutation using ambient mass spectrometry imaging (MSI). Materials methods: MSI were...
Intrahepatic cholangiocarcinoma (ICC) is a liver tumor featured by challenges of non-invasive early diagnosis and higher prevalence rate in Asian countries. These characteristics necessitate the development liquid biopsy immunotherapy methods to improve prognosis patients with ICC. Herein, we conducted pilot study on transcriptome tissues, adjacent normal plasma exosomes ICC from northern southern China. We identified subgroup immunogenic ICC, which different Caucasian characterized T cell...
Abstract Purpose The optimal treatment after neoadjuvant chemoimmunotherapy for patients with stage III non-small cell lung cancer (NSCLC) is unclear. This study aimed at comparing the efficacy and safety of chemoradiotherapy surgery in NSCLC. Materials methods We conducted a real-world multicenter retrospective on NSCLC who received or between October 2018 December 2022. Progression-free survival (PFS) overall (OS) were assessed from initiation estimated by Kaplan‒Meier method. Univariate...