A5018995173- Sarcoma Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Ubiquitin and proteasome pathways
- Nuclear Structure and Function
- HER2/EGFR in Cancer Research
- Genomics and Chromatin Dynamics
- Signaling Pathways in Disease
- Chronic Lymphocytic Leukemia Research
- Melanoma and MAPK Pathways
- Bone Tumor Diagnosis and Treatments
- Vascular Tumors and Angiosarcomas
- Management of metastatic bone disease
- Cancer Diagnosis and Treatment
- Bone health and treatments
- Soft tissue tumor case studies
- Musculoskeletal synovial abnormalities and treatments
- Neurofibromatosis and Schwannoma Cases
- Molecular Biology Techniques and Applications
- Medical Imaging and Pathology Studies
- BRCA gene mutations in cancer
- Urologic and reproductive health conditions
- Colorectal Cancer Treatments and Studies
- Cardiac tumors and thrombi
Tokyo Metropolitan Komagome Hospital
2013-2024
The University of Tokyo
2017-2022
National Cancer Research Institute
2021
Fujisawa City Hospital
2021
University of Tokyo Hospital
2016-2020
Japan Society for the Promotion of Science
2020
Japan Agency for Medical Research and Development
2020
Daiichi Sankyo Foundation of Life Science
2020
Takeda Science Foundation
2020
Committee on Publication Ethics
2020
In Brief Study Design. A retrospective, radiographical study. Objective. To evaluate short-term radiological changes in sagittal alignment after lumbar decompression without fusion for canal stenosis. Summary of Background Data. Although the importance global balance is underscored recently, little known about decompression. Methods. We retrospectively reviewed 88 patients who underwent at a single institution between November 2008 and May 2013, with minimum follow-up 5 months. Standing...
Tumor molecular profiling is becoming a standard of care for patients with cancer, but the optimal platform cancer sequencing remains undetermined. We established comprehensive assay, Todai OncoPanel ( TOP ), which consists DNA and RNA hybridization capture‐based next‐generation panels. A novel method target enrichment, named junction capture method, was developed panel to accurately cost‐effectively detect 365 fusion genes as well aberrantly spliced transcripts. The can also measure...
Abstract Mutations in ERBB2 (encoding HER2) occur 2% to 4% of non–small cell lung cancer (NSCLC) and confer poor prognosis. ERBB-targeting tyrosine kinase inhibitors, approved for treating other HER2-dependent cancers, are ineffective HER2-mutant NSCLC due dose-limiting toxicities or suboptimal potency. We report the discovery zongertinib (BI 1810631), a covalent HER2 inhibitor. Zongertinib potently selectively blocks HER2, while sparing EGFR, inhibits growth cells dependent on oncogenic...
Numerous nontruncating missense variants of the BRCA2 gene have been identified, but there is a lack convincing evidence, such as familial data, demonstrating their clinical relevance and they thus remain unactionable. To assess pathogenicity unknown significance (VUSs) within BRCA2, here we develop method, MANO-B for high-throughput functional evaluation utilizing BRCA2-deficient cells poly (ADP-ribose) polymerase (PARP) inhibitors. The estimated sensitivity specificity this assay compared...
Various genetic alterations of the fibroblast growth factor receptor (FGFR) family have been detected across a wide range cancers. However, inhibition FGFR signaling by kinase inhibitors demonstrated limited clinical effectiveness. Herein, we evaluated transforming activity and sensitivity 160 nonsynonymous mutations ten fusion genes to seven tyrosine (TKI) using mixed-all-nominated-in-one (MANO) method, high-throughput functional assay. The oncogenicity 71 mutants was newly discovered in...
Activating mutations in mitogen-activated protein kinase 1 (MAP2K1) are involved a variety of cancers and may be classified according to their RAF dependence. Sensitivity combined BRAF MEK treatments is associated with co-mutations MAP2K1 BRAF; however, the significance less frequent largely unknown. The transforming potential drug sensitivity 100 variants were evaluated using individual assays mixed-all-nominated-in-one method. In addition, A375, melanoma cell line harboring V600E mutation,...
<p>PRISM screen raw data.</p>
<div>Abstract<p>Mutations in <i>ERBB2</i> (encoding HER2) occur 2% to 4% of non–small cell lung cancer (NSCLC) and confer poor prognosis. ERBB-targeting tyrosine kinase inhibitors, approved for treating other HER2-dependent cancers, are ineffective HER2-mutant NSCLC due dose-limiting toxicities or suboptimal potency. We report the discovery zongertinib (BI 1810631), a covalent HER2 inhibitor. Zongertinib potently selectively blocks HER2, while sparing EGFR, inhibits...
<p>High-throughput zongertinib PRISM drug sensitivity screen and biomarker analysis.</p>
<p>High-throughput PRISM drug sensitivity screen in cancer cell lines.</p>
<p>Tempus data analysis results.</p>
<p>In vitro biomarker modulation of (phospho-)protein and mRNA levels post poziotinib or zongertinib treatment.</p>
<p>Graphical NMR spectra of zongertinib and BI-3999</p>
<p>MANO method and results. Sheet 1: Primer sequence for the bar code amplification. 2: next-generation sequencing analysis. 3: Raw data zongertinib (BI 1810631 in table) drug sensitivity assay.</p>
<p>In vivo tumor responses for zongertinib monotherapy or combinations.</p>
<p>Tolerability data for zongertinib in mouse xenotransplantation assays.</p>
<p>Synthesis and in vivo DMPK profile of zongertinib</p>
Abstract Background: Osteosarcoma (OS), the most common primary malignant bone tumor, presents challenges in treatment, especially metastatic or recurrent cases. Genomic profiling may offer novel therapeutic approaches by identifying actionable mutations. This study utilizes data from Japanese Center for Cancer Genomics and Advanced Therapeutics (C-CAT) AACR Project GENIE, comparing findings with MSK-IMPACT dataset to understand differences genomic landscapes identify clinically alterations....
The RCK gene is a target of the t(11;14)(q23;q32) chromosomal translocation observed in human B-cell lymphoma, and overexpression its protein (rck/p54) by was shown to cause malignant transformation. rck/p54 belongs DEAD box protein/RNA helicase family, which has variety functions such as translation initiation, pre-mRNA splicing ribosome assembly. expression rck p54 colorectal adenocarcinoma cells examined immunohistochemistry Western blot analysis. found be overexpressed tumour tissues...
Schwannomas are well-encapsulated, benign neoplasms, and enucleation is a standard operation procedure. The incidence of neurological complications after surgical treatment for schwannomas the extremities varies, there no consensus concerning predictive factors complications. aim this study was to elucidate that develop in major nerves extremities. A total 139 patients with 141 arising were retrospectively analyzed. Data regarding preoperative clinical features, postoperative complications,...
Patients with advanced cancer undergo comprehensive genomic profiling in Japan only after treatment options have been exhausted. a very poor prognosis were not able to tests, resulting selection bias called length bias, which makes accurate survival analysis impossible. The actual impact of on the overall patients who undergone tests is unclear, yet appropriate methods for adjusting developed. To assess survival, we established simulation-based model adjustment. This study utilized...
Although desmoplastic fibroblastoma (DFB) and fibroma of tendon sheath (FTS) are well-established entities, they may show overlapping clinicopathological features. In addition, cytogenetic data showing a shared 11q12 rearrangement in small number cases suggest close link between these entities. A recent microarray study revealed up-regulation FOSL1 mRNA DFBs with rearrangement. The aim this was to clarify the relationship DFB FTS.We tested 42 diagnosed originally as either or FTSs for...
ObjectivesExon 20 insertion mutations of epidermal growth factor receptor (EGFR) have been identified as oncogenic in general; however, the functional relevance each remains largely uninvestigated. Herein, we comprehensively assessed significance EGFR exon 20.Materials and methodsThe transforming potential drug sensitivities 25 recurrent mutants, including twenty-one insertions, were evaluated using mixed-all-nominated-in-one method.ResultsThe sensitivity insertions to tyrosine kinase...