A5010163028- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- Circular RNAs in diseases
- PARP inhibition in cancer therapy
- Bacteriophages and microbial interactions
- Galectins and Cancer Biology
- Advanced Biosensing Techniques and Applications
- Calcium signaling and nucleotide metabolism
- Herpesvirus Infections and Treatments
- Cancer Mechanisms and Therapy
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Healthcare and Venom Research
- Brucella: diagnosis, epidemiology, treatment
- Systemic Lupus Erythematosus Research
- Salmonella and Campylobacter epidemiology
- Natural Antidiabetic Agents Studies
- Metabolism, Diabetes, and Cancer
- Growth Hormone and Insulin-like Growth Factors
- Antimicrobial Peptides and Activities
- Streptococcal Infections and Treatments
- Multiple Sclerosis Research Studies
- Neurogenesis and neuroplasticity mechanisms
- Microbial Inactivation Methods
- Diabetes Treatment and Management
Jilin University
2024
Jilin Academy of Agricultural Sciences
2023
Chinese Academy of Agricultural Sciences
2021-2023
Academy of Military Medical Sciences
2018-2020
Samsung Medical Center
2004
Sungkyunkwan University
2004
Melittin, a main component of bee venom, is cationic amphiphilic peptide with linear α-helix structure. It has been reported that melittin can exert pharmacological effects, such as antitumor, antiviral and anti-inflammatory effects in vitro vivo . In particular, may be beneficial for the treatment diseases which no specific clinical therapeutic agents exist. Melittin effectively enhance properties some first-line drugs. Elucidating mechanism underlying melittin-mediated biological function...
ABSTRACT Main Problem Glioblastoma (GB) is one of the most prevalent and devastating types brain cancer for which efficient treatments are currently lacking because limitations such as antitumor efficacy, delivery, tumor selectivity, drug resistance. A promising strategy to overcome these obstacles developing anticancer agents that can be delivered GB tissues inhibit tumors with low toxicity normal tissue. Methods We developed liposomes encapsulating resveratrol (RES), a polyphenolic...
Type 2 diabetes constitutes a serious threat to the health of patients, but there is currently no ideal treatment in clinic. Glucagon-like peptide-1 and human umbilical cord mesenchymal stem cells have been confirmed antidiabetic effects, both them certain defects process antidiabetes, which cannot meet need clinical treatment. We hypothesized that can be used as vector construct novel cell line expresses GLP-1 vivo for long time. And this strain results lowering blood glucose type diabetic...
Herein, antibody–HRP co-assembled nanocomposites with enhanced stability were successfully fabricated and employed as an alternative to traditional enzyme-labeled antibodies develop a chemiluminescence enzyme immunoassay toward E. coli O157:H7.
Introduction The constantly mutating SARS-CoV-2 has been infected an increasing number of people, hence the safe and efficacious treatment are urgently needed to combat COVID-19 pandemic. Currently, neutralizing antibodies (Nabs), targeting receptor-binding domain (RBD) spike protein potentially effective therapeutics against COVID-19. As a new form antibody, bispecific single chain (BscAbs) can be easily expressed in E. coli exhibits broad-spectrum antiviral activity. Methods In this study,...
Ricin toxin binding subunit B (RTB) is a galactose-binding lectin protein derived from the beans of castor oil plant (Ricinus communis). Our previous studies have reported direct immunomodulatory effect recombinant RTB, which stimulates RAW264.7 cells to produce cytokines including TNF-α. However, RTB on innate immune response and its mechanism are not well characterized. In present study, results showed that treatment macrophages significantly increased TLR4 levels. also activated...
Increasing studies have concentrated on investigating circular RNAs (circRNAs) as pivotal regulators in the progression of numerous diseases and biological processes abundant evidence shows that circRNAs are participated regulation innate immune responses. Several showed Ricin Toxin (RT) could induce inflammatory injury. There was no research particular functions underlying mechanisms RT-induced inflammation. In this study, RNA sequencing performed RT-treated normal RAW264.7 macrophage cells...
Summary The purpose of this study is to establish a rapid and accurate method based on multiplex polymerase chain reaction ( PCR ) combined with suspension array xTAG (Flexible Sequence‐tagged, technology simultaneously detect Salmonella Typhimurium, Brucella spp., Bacillus cereus Shigella spp. in raw milk. primers were designed the specific target gene four pathogenic bacteria, then TAG sequence was used modify primers. First, products hybridised microspheres labelled anti‐ , they...
Ricin toxin (RT), extracted from castor bean, is a type II ribosome-inactivating protein (RIP). As biothreat agent, there no effective antidote for RT to date, but recent advances in antibody research may have meet this need. This study aimed produce human single-chain variable fragments (HuscFvs) that bind and interfere with activity further clinical use. The purified component was used phage biopanning select ricin-bound HuscFv-displayed clones synthetic (Tomlinson I + J) display...
The current study aimed at investigating the therapeutic effects of rubusoside on type 2 diabetes mellitus (T2DM) mice models as an alternative hypoglycemic candidate drug. T2DM were established with a combination streptozotocin (STZ) intraperitoneal injection and high-fat diet. After 10 weeks intragastric administration (100, 200 mg/kg/day) to mice, body weight, fasting blood glucose, glucose tolerance, lipids measured. liver protein expression levels p-AMPK, GLUT2, GLUT4 total antioxidant...
Despite interferon alpha having a broad spectrum of antiviral activity and strong antiproliferative activity, its applications are severely limited due to the intrinsic properties proteins, such as poor stability short serum half-life. In our study, canine (CaIFNα) gene was fused with ricin toxin B chain (RTB) form rCaIFNα/RTB, which encodes 463-amino acid protein containing 15-amino encoded (G4S)3 flexible linker. After expression in prokaryote, purification renaturation, cytotoxicity...
Mitochondrial dysfunction and oxidative stress play essential roles in the pathogenesis of diabetic nephropathy (DN). The respiratory oxygen consumption status kidney mitochondria are closely associated with development DN. In this study, raspberry ketone (RK), predominant bioactive component extracted from raspberry, was applied to treat established DN mice model. This study investigated whether RK protects kidneys high-fat high-sugar/streptozotocin (STZ)-induced rats by inhibiting...
Abstract Background: Neuromyelitis optica spectrum disorder (NMOSD) is an immune-mediated inflammatory demyelinating disease and AQP4 antibody (AQP4-IgG or NMO-IgG) the major cause of NMOSD. However，Many underlying molecular mechanisms in occurrence development NMOSD are not completely understood. Insulin-like growth factor-2 (IGF-2) a multifunctional cell proliferation regulator which plays essential role early embryonic development. Recent studies have shown that IGF-2 has crucial effect...
P942 Aims: CD45, a protein tyrosine phosphatase, is positive regulator in T and B lymphocytes for their development activation. CD45 exists as multiple isoforms among which CD45RB regulated dynamic during cell Therefore, immunotherapy using monoclonal antibodies (mAb) specific CD45RB, considered potent tolerogenic target, may be applied tolerance induction transplantation. We demonstrated that anti-CD45RB mAb when combined with anti-CD154 (MR1) show synergy costimulatory blockade (CTLA4-Ig...