A5002816480- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Bacterial Identification and Susceptibility Testing
- Thermal Regulation in Medicine
- Digital Holography and Microscopy
- Photoacoustic and Ultrasonic Imaging
- Digital Imaging for Blood Diseases
- Blood disorders and treatments
- Microfluidic and Bio-sensing Technologies
- Hematological disorders and diagnostics
- Optical Coherence Tomography Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Thermography and Photoacoustic Techniques
- Neutropenia and Cancer Infections
- Non-Invasive Vital Sign Monitoring
- Cellular Mechanics and Interactions
- Functional Brain Connectivity Studies
- Field-Flow Fractionation Techniques
- Electrohydrodynamics and Fluid Dynamics
- Infrared Thermography in Medicine
- Single-cell and spatial transcriptomics
- Peripheral Artery Disease Management
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Diabetic Foot Ulcer Assessment and Management
The Wallace H. Coulter Department of Biomedical Engineering
2022-2025
Georgia Institute of Technology
2022-2025
Emory University
2023
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2020-2021
National Institutes of Health
2020-2021
Deep-ultraviolet (UV) microscopy enables label-free, high-resolution, quantitative molecular imaging and unique applications in biomedicine, including the potential for fast hematological analysis at point-of-care. UV has been shown to quantify hemoglobin content white blood cells (five-part differential), providing a simple alternative current gold standard, analyzer. Previously, however, system comprised bulky broadband laser-driven plasma light source along with large expensive camera 3D...
Cell therapies, including T cell immunotherapies, offer promising treatments for previously untreatable diseases, but their widespread use is hindered by challenges in monitoring therapeutic cells during culture -- impacting consistency, potency, and cost. This work demonstrates the of quantitative phase imaging (QPI), specifically a compact, non-interferometric form called oblique back illumination microscopy (qOBM), non-destructive, label-free, in-line assessment cultures. qOBM enables...
T cell characterization is critical for understanding immune function, monitoring disease progression, and optimizing cell-based therapies. Current technologies to characterize cells, such as flow cytometry, require fluorescent labeling typically are destructive endpoint measurements. Non-destructive, label-free imaging methods have been proposed, but face limitations with throughput, specificity, system complexity. Here we demonstrate deep-ultraviolet (UV) microscopy a label-free,...
Neutropenia is a condition comprising an abnormally low number of neutrophils, type white blood cell, which puts patients at increased risk severe infections. especially common among cancer and can disrupt their treatment or even be life-threatening in cases. Therefore, routine monitoring neutrophil counts crucial. However, the current standard care to assess neutropenia, complete count (CBC), resource-intensive, time-consuming, expensive, thereby limiting easy timely access critical...
Ultraviolet (UV) microscopy of live cells has been challenging due to phototoxicity, with UV radiation affecting cellular components leading irreversible cell death. Despite this challenge, recent advances in light sources and detectors have renewed interest its high resolution label-free molecular imaging capabilities. Indeed, recently demonstrated for a wide variety applications, including multispectral cancer tissue sections, at varying time scales, hematological analysis whole blood...
Deep-UV microscopy enables high-resolution, label-free molecular imaging by leveraging biomolecular absorption properties in the UV spectrum. Recent advances UV-imaging hardware have renewed interest this technique for quantitative live cell applications. However, UV-induced photodamage remains a concern longitudinal dynamic studies. Here, we quantify phototoxicity with several types at notable wavelengths. We find that fluence required death via continuous exposure varies type and...
Intracellular activity occurs at a wide range of time scales, requiring multiscale approach for data acquisition and analysis. Current optical techniques used imaging intracellular dynamics suffer from limited speeds, low biomolecular specificity, or require fluorescent labels which can perturb cellular environments. Deep-ultraviolet (UV) microscopy enables fast, label-free, quantitative molecular with subcellular resolution. Previously, multispectral deep-UV has been demonstrated robust...
Bone marrow aspiration procedures play an important role in the assessment of patients with blood and diseases, including cancers. Evaluating adequacy aspirates, indicated by presence bony spicules, is crucial to ensure procedural success collection relevant diagnostic material. Unfortunately, inadequate samples occur approximately 50 % cases, requiring undergo repeat procedures. This particularly problematic for pediatric who need be anesthetized before each procedure. The current gold...
DFFOCT allows for the visualization and characterization of cellular dynamics, providing future direction cell-targeted therapeutics. In this work, we analyze potential to increase understanding dynamic processes under varying conditions.
Deep Ultraviolet (UV) Microscopy enables high-resolution, quantitative, and label-free imaging of biomolecules. Recently, we have shown that UV microscopy can be used as a tool for simple fast hematology analysis by providing diagnostically relevant information on morphological cytogenic properties various blood cells. We demonstrated the ability to classify segment red cells, white cell subtypes, platelets via deep learning frameworks applied this technique diagnosis disorders. In work,...
Dynamic Full-Field Optical Coherence Tomography (D-FFOCT) is a high transverse resolution version of OCT that records signal at the output interferometer as movie over few seconds. Analyzing temporal variation signals reveals intracellular contrast which gives D-FFOCT capability identifying dynamic metabolic changes. We use to identify oxygen induced changes in cellular metabolism ambient (22%) oxygenation and hypoxia (1%). Signal strength, calculated an integral raw FFT spectrum,...
Working memory (WM) is part of the short-term storage in human brain. N-back a WM task to assess mental workload on prefrontal cortex (PFC). In this work, we studied how changes an over length experiment. We focused two levels two- and three-back. Examining both hemodynamics behavioral data (correct answers), found significant difference between 2-back 3-back tasks beginning end task.
We developed a wearable system for wireless monitoring of oxygenation deep tissues such as liver and lung during exercise. It is also useful where subcutaneous fat thickness high. Our utilizes Continuous Wave Near Infrared Spectroscopy (CW NIRS) with source-detector distances from 10mm to 60mm. This allows us observe at various depths. To mitigate the interference overlaying tissue layers skin, muscle, we multi-layer Monte Carlo model photon diffusion. Flexible structure our device helps...
Neutropenia is a blood disorder characterized by an abnormally low number of neutrophils in the bloodstream. This condition signifies increased risk infections, and thus can lead to life-threatening medical emergencies severe cases. Therefore, it critical routinely monitor neutrophil counts cancer patients. However, current clinical standard-of-care for cell enumeration assess neutropenia relies on complete count (CBC) which requires expensive complex equipment, multiple reagents, cumbersome...