A5100439847- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- DNA and Nucleic Acid Chemistry
- Parasitic Infections and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Metabolomics and Mass Spectrometry Studies
- Parasites and Host Interactions
- Amoebic Infections and Treatments
- interferon and immune responses
- Aquaculture disease management and microbiota
- Immune Cell Function and Interaction
- Neuropeptides and Animal Physiology
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Biosimilars and Bioanalytical Methods
- Pharmacological Effects of Natural Compounds
- Pharmacogenetics and Drug Metabolism
- Advanced Biosensing Techniques and Applications
- Phytochemistry and Biological Activities
- Analytical Chemistry and Chromatography
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
Shenzhen Third People’s Hospital
2024
Huazhong Agricultural University
2024
Biogen (United States)
2019-2024
Vertex Pharmaceuticals (United States)
2024
Stomatology Hospital
2023
Ningbo No. 2 Hospital
2023
Nanjing Drum Tower Hospital
2023
Northwest A&F University
2023
Wuhan Academy of Agricultural Sciences
2019-2022
Chinese Academy of Tropical Agricultural Sciences
2019-2022
Controversies exist on the potential role of companion animals in transmission enteric pathogens humans. This study was conducted to examine genotype distribution Cryptosporidium spp., Enterocytozoon bieneusi, and Giardia duodenalis Shanghai, China, assess their zoonotic potential.Fecal specimens from 485 dogs 160 cats were examined for occurrence three by PCR. PCR products sequenced determine species genotypes. The χ(2) test used compare differences infection rates between living conditions...
Interleukin receptor-associated kinase 4 (IRAK4) is a key node of signaling within the innate immune system that regulates production inflammatory cytokines and chemokines. The presence
Quantitative bioanalysis in plasma and tissues samples is required to study the pharmacokinetic pharmacodynamic properties of antisense oligonucleotides (ASOs). To overcome intrinsic drawbacks specificity, sensitivity, throughput traditional ligand-binding assay (LBA) liquid chromatography–tandem mass spectrometry (LC–MS/MS) methods, an alternative bioanalytical method was developed by combining oligonucleotide hybridization LC–MS/MS technologies. Target ASOs were extracted from biological...
A Correction to this paper has been published: https://doi.org/10.1038/s41467-021-22614-1.
Background: Antisense oligonucleotide (ASO), an emerging modality in drug research and development, demands accurate sensitive bioanalysis to understand its pharmacokinetic pharmacodynamic properties. Results: By combining the advantages of both ligand binding liquid chromatography-mass spectrometry/tandem mass (LC-MS/MS), hybridization LC-MS/MS methods were successfully developed validated/qualified a good lab practice (GLP) environment for quantitation ASO candidate monkey serum,...
Periodontitis is a highly prevalent oral disease characterized by irreversible bone resorption and tooth loss. The proliferation, migration osteogenic differentiation of periodontal ligament stem cell (PDLSC) are crucial to the regeneration defects. There increasing evidence that small extracellular vesicle (sEV) derived from pulp cell, including human exfoliated deciduous teeth (SHED) dental (DPSC), potential mediator for tissue regeneration. However, which one more suitable formation still...
Lung cancer has the highest mortality rate amongst all malignancies worldwide, and is second-highest incidence of in women. Non-small cell lung (NSCLC) responsible for approximately 80% cases. Recent studies indicate that cellular senescence may be a promising biomarker. However, regulation its underlying mechanisms NSCLC are not yet fully understood. Here, we present comprehensive analysis genes linked to NSCLC. We also describe secretory phenotype associated with examine immune profile...
We herein report the discovery, synthesis, and evolution of a series indazoles azaindazoles as CNS-penetrant IRAK4 inhibitors. Described is use structure-based property-based drug design strategically leveraged to guide property profile key into favorable space while maintaining potency selectivity. Our rationale that led toward functionalities with improvements, CNS-penetration, solubility, drug-like properties portrayed. In vivo evaluation an advanced analogue showed significant,...
Interleukin receptor associated kinase 4 (IRAK4) plays an important role in innate immune signaling through Toll-like and interleukin-1 receptors represents attractive target for the treatment of inflammatory diseases cancer. We previously reported development a potent, selective, brain-penetrant imidazopyrimidine series IRAK4 inhibitors. However, lead molecule BIO-7488 (1) suffered from low solubility which led to variable PK, compound accumulation, poor vivo tolerability. Herein, we...
Antisense oligonucleotides (ASOs) have been conjugated to various moieties, such as peptides, antibodies or Fab regions of antibodies, enhance their delivery target tissues. The quantitation free ASO (ASO payload) is critical characterize its pharmacokinetics/pharmacodynamics (PK/PD) properties and biodistribution after the peptide/antibody/Fab conjugates.
Abstract Triple-negative breast cancer (TNBC), an aggressive histological subtype of cancer, exhibits a high risk early recurrence rate and poor prognosis, it is primarily associated with the abundance stem cells (CSCs). At present, strategies for effectively eradicating or inhibiting TNBC CSCs are still limited, which makes development novel drugs anti-CSCs function be great value treatment TNBC, especially refractory TNBC. In this study, we found that small-molecule tyrosine kinase...
In East Asia, the dark sleeper, Odontobutis obscura (O. obscura) is a crucial commercial species of freshwater fish; however, its molecular biology research still undeveloped, including innate immune system, which pivotal to antiviral response. this study, we cloned and identified characterization kinase function sleeper TANK-binding 1 (TBK1), supplementing evidence conservation classical factor in fish. First, ORF obscurus TBK1 (OdTBK1) was from liver tissue by RACE-PCR. Subsequent nucleic...
Ardisiae Japonicae Herba is a well-known traditional Chinese medicine for the treatment of bronchitis conjunctivitis, pneumonia, and trauma. In this work, high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was first established separation structural identification chemical constituents in Herba. A total 15 compounds including coumarins, flavonoid glycosides, catechins were identified or tentatively characterized based on their...
Pasteurella multocida is a pathogenic microorganism that causes variety of serious diseases in humans and animals worldwide. The global regulator gene, fur, plays an important role pathogenesis regulates the virulence many bacteria. Here, we identified fur gene P. by complementing Salmonella Choleraesuis Δfur mutant, characterized mutant strain multocida. exhibited no significant differences growth outer membrane protein (OMP) profiles when complemented was compared to parent. Ducks were...
Abstract Carbapenemase-producing E. coli is a grave public health concern as the potential emergence of resistant strains and their transmission. Isoorientin belongs to antimicrobial flavonoid compound existing in several plants, while research on activity isoorientin limited thus far. We evaluated antibiofilm effects against biofilm-forming carbapenem non-sensitive Escherichia (E. coli) from raw milk goats, explored its molecular mechanisms. showed obvious ability with minimum inhibitory...