A5101535857- Traditional Chinese Medicine Studies
- Bone and Dental Protein Studies
- Rheumatoid Arthritis Research and Therapies
- Cytokine Signaling Pathways and Interactions
- Natural Compounds in Disease Treatment
- Biomedical Text Mining and Ontologies
- Pharmacological Effects of Natural Compounds
- Metabolomics and Mass Spectrometry Studies
- Nitric Oxide and Endothelin Effects
- dental development and anomalies
- Traditional Chinese Medicine Analysis
- Bone Metabolism and Diseases
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
- Healthcare and Venom Research
- Acupuncture Treatment Research Studies
- RNA modifications and cancer
- MicroRNA in disease regulation
- Protein Tyrosine Phosphatases
- Digestive system and related health
- Hepatitis C virus research
- Cancer Cells and Metastasis
- Computational Drug Discovery Methods
- Peroxisome Proliferator-Activated Receptors
- Adenosine and Purinergic Signaling
First Affiliated Hospital of Henan University of Traditional Chinese Medicine
2015-2024
Inner Mongolia Medical University
2022-2024
First Affiliated Hospital of Henan University
2022-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2010-2023
Yan'an University
2011-2023
First Affiliated Hospital of Xi'an Jiaotong University
2023
China Aerodynamics Research and Development Center
2023
Henan University
2013-2022
Beike Biotechnology (China)
2022
Chang'an University
2022
The interaction between cancer and its local microenvironment can determine properties of growth metastasis. A critical component the tumor in this context is cancer-associated fibroblast (CAF), which promote growth, angiogenesis It has been hypothesized that CAF may be derived from mesenchymal stromal cells (MSC), or distant sources. However, signaling mechanisms by tumors MSCs interact to CAF-dependent are largely unknown. In study with vitro vivo models using MDA-MB231 human breast cells,...
Osteopontin (OPN) is a secreted phosphoprotein which mediates tumorigenesis, local growth, and metastasis in variety of cancers. It potential therapeutic target for the regulation cancer metastasis. RNA aptamer technology targeting OPN may represent clinically viable therapy. In this study, we characterize critical sequence an aptamer, termed OPN-R3, directed against human OPN. has Kd 18 nmol/l binds specifically to as determined by electrophoretic mobility assays. MDA-MB231 breast cells...
Quantitative studies using validated questionnaires on post-traumatic stress disorder (PTSD) of Nurses exposed to corona virus disease 2019 (COVID-19) in China are rare and the baseline PTSD must first be evaluated before prevention. This study aimed investigate factors potentially involved level COVID-19 China.In this cross-sectional study, male female (n = 202) from HuBei were included final sample. The Checklist-Civilian (PCL-C) questionnaire Simplified Coping Style Questionnaire (SCSQ)...
Abstract In a system of endotoxin (LPS)-mediated NO production in ANA-1 murine macrophages, suppression subtractive hybridization was used to identify genes up-regulated by NO. Osteopontin (OPN), secreted acidic phosphoprotein that binds cell surface RGD integrin-binding motif, found be differentially expressed the presence OPN has been demonstrated inhibit variety types. Northern blot and nuclear run-on analyses mRNA levels gene transcription were significantly increased LPS-induced...
Current strategies for drug discovery have reached a bottleneck where the paradigm is generally "one gene, one drug, disease." However, using holistic and systemic views, network pharmacology may be next in discovery. Based on pharmacology, combinational with two or more compounds could offer beneficial synergistic effects complex diseases. Interestingly, traditional chinese medicine (TCM) has been practicing views over 3,000 years, its distinguished feature herbal formulas to treat diseases...
Metastasis-supporting physiological alterations are regulated by cell signaling molecules, which target signal transduction pathways and gene expression. Osteopontin (OPN) overexpression may represent a key molecular event in cancer metastasis. In this study, using metastatic 4T1 non-metastatic 4T07 murine mammary lines, we demonstrate that cells exhibit significantly increased OPN, integrin-linked kinase (ILK), matrix metalloproteinase-2 (MMP-2) urokinase-type plasminogen activator (uPA)...
Zheng, which is also called a syndrome or pattern, the basic unit and key concept of traditional Chinese medicine (TCM) theory. Zheng can be considered further stratification patients when it integrated with biomedical diagnoses in clinical practice to achieve higher efficacies. In an era evidence-based medicine, confronted vast increasing volume TCM data, there urgent need explore these resources effectively using techniques knowledge discovery databases. The application effective data...
Abstract Tripterygium wilfordii Hook F. (TwHF) based therapy has been proved as effective in treating rheumatoid arthritis (RA), yet the predictors to its response remains unclear. A two-stage trial was designed identify and verify baseline symptomatic of this therapy. 167 patients with active RA were enrolled a 24-week TwHF treatment identified an open trial; then randomized clinical (RCT) for verification, 218 classified into predictor positive (P+) negative (P−) group randomly assigned...
Abstract Ovarian cancer is a common gynecological malignant tumor with high mortality rate and poor prognosis. There inadequate knowledge of the molecular mechanisms underlying ovarian cancer. We examined expression methyltransferase-like 3 (METTL3) in specimens using RT-qPCR, immunohistochemistry, Western blot analysis, tested methylation METTL3 by MSP. Levels METTL3, miR-1246, pri-miR-1246 CCNG2 were then analyzed their effects on cell biological processes also investigated, vivo assay to...
Osteopontin (OPN) is a sialic acid-rich phosphoprotein secreted by wide variety of cancers. We have shown previously that OPN necessary for mediating hepatic metastasis in CT26 colorectal cancer cells. Although stimuli can induce OPN, the molecular mechanisms regulate gene transcription are unknown. hypothesized cis- and trans-regulatory elements determine was analyzed cells compared with YAMC (young adult mouse colon) epithelial Clonal deletion analysis promoter-luciferase constructs...
IN TRADITIONAL CHINESE MEDICINE (TCM), PATIENTS WITH RHEUMATOID ARTHRITIS (RA) CAN BE CLASSIFIED INTO TWO MAIN PATTERNS: cold-pattern and heat-pattern. This paper identified the network-based gene expression biomarkers for both cold- heat-patterns of RA. Gene profilings CD4+ T cells from RA patients, heat-pattern healthy volunteers were obtained using microarray. The differentially expressed genes related networks explored DAVID, GeneSpring software, protein-protein interactions (PPI)...
Abstract Small cell lung cancer (SCLC) is one of the most lethal cancer, mainly attributing to its high tendency metastasis. Mounting evidence has demonstrated that genes and microRNAs (miRNAs) are related human onset progression including invasion An eligible gene dataset an miRNA were downloaded from Gene Expression Omnibus (GEO) database based our screening criteria. Differentially expressed (DE-genes) or DE-miRNAs for each obtained by R software package. The potential target top 10...
Abstract Metformin (Met) is a commonly used drug in the treatment of type 2 diabetes. Currently, it has been found that Met can effectively reduce incidence stroke and exert anti‐inflammatory effects. However, its role ischemia‐reperfusion (I/R)‐induced nerve injury remains unclear. This study aims to investigate neuroprotective effects I/R‐induced neuron as well underlying mechanism. A middle cerebral artery occlusion (MCAO) model was established Sprague Dawley (SD) rats, which were then...
Abstract Osteopontin is a glycoprotein that has been linked to metastatic function in breast, lung, and prostate cancers. However, the mechanism by which osteopontin acts induce properties largely unknown. One intriguing feature of presence conserved thrombin cleavage site COOH-terminal from well-characterized RGD domain. Although fragment may bind cell surface CD44 receptors, little known about fragment. In current study, we use murine mammary epithelial tumor lines 4T1 4T07; these cells...
One important concept in traditional Chinese medicine (TCM) is "treating different diseases with the same therapy". In TCM practice, some patients Rheumatoid Arthritis (RA) and other Coronary Heart Disease (CHD) can be treated similar therapies. This suggests that there might something commonly existed between RA CHD, for example, biological networks or basis. As amount of biomedical data leading databases (i.e., PubMed, SinoMed, etc.) growing at an exponential rate, it possible to get...
To determine the efficacy of osteopontin (OPN) targeting in hepatocellular cancer (HCC). SUMMARY/BACKGROUND: OPN is associated with HCC growth and metastasis represents a unique therapeutic target.OPN epithelial-mesenchymal transition (EMT) markers, α-smooth muscle actin (SMA), vimentin, tenascin-c, were measured archived human tissues from metastatic (n = 4) nonmetastatic settings. Additional studies utilized Sk-Hep-1 (high expression) Hep3b (low cells. An RNA aptamer (APT) that avidly...