A5057966768- Animal Behavior and Welfare Studies
- Liver physiology and pathology
- Wound Healing and Treatments
- Genomics, phytochemicals, and oxidative stress
- Animal Genetics and Reproduction
- Genetic and phenotypic traits in livestock
- Fibroblast Growth Factor Research
- Antimicrobial Peptides and Activities
- Redox biology and oxidative stress
- Skin Protection and Aging
- Animal testing and alternatives
- Protease and Inhibitor Mechanisms
- Glutathione Transferases and Polymorphisms
- Medical and Biological Ozone Research
- Growth Hormone and Insulin-like Growth Factors
- Drug-Induced Hepatotoxicity and Protection
- Angiogenesis and VEGF in Cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hair Growth and Disorders
- Genetic Mapping and Diversity in Plants and Animals
- Pluripotent Stem Cells Research
- Ethics in medical practice
- Skin and Cellular Biology Research
- Connective Tissue Growth Factor Research
- Cell Adhesion Molecules Research
University of Zurich
2013-2023
Institute of Laboratory Animal Science
2021
ETH Zurich
2001-2008
Institute of Cell Biology
2000-2007
National Institute of Allergy and Infectious Diseases
2004
National Institutes of Health
2004
To gain insight into the molecular mechanisms underlying cutaneous wound repair, we performed a large scale screen to identify novel injury-regulated genes. Here show strong up-regulation of RNA and protein levels two Ca<sup>2+</sup>-binding proteins S100A8 S100A9 in hyperthickened epidermis acute murine human wounds ulcers. Furthermore, both genes were expressed by inflammatory cells wound. The increased expression keratinocytes is most likely related activated state not secondary...
The liver is frequently exposed to insults, including toxic chemicals and alcohol, viral infection or metabolic overload. Although it can fully regenerate after acute injury, chronic damage causes fibrosis cirrhosis, which result in complete failure. In this study, we demonstrate that the NF-E2-related factor 2 (Nrf2) transcription protects from toxin-mediated damage. Repair of injury occurs a single treatment with hepatotoxin carbon tetrachloride (CCl(4)) was severely delayed Nrf2-deficient...
Endostatin is a cleavage product of collagen XVIII that strongly inhibits tumor angiogenesis. To determine if endostatin affects other angiogenic processes, we generated full-thickness excisional wounds on the back mice were systemically treated with recombinant murine endostatin. No macroscopic abnormalities wound healing process observed. Histological analysis revealed normal contraction and re-epithelialization, but slight reduction in granulation tissue formation reduced matrix...
The matrix metalloproteinase stromelysin-2 is expressed in keratinocytes of the epithelial tongue skin wounds, suggesting a role keratinocyte migration. Here, we show that enhances migration cultured keratinocytes. To gain insight into vivo activities repair, generated transgenic mice expressing constitutively active mutant These animals had no alterations architecture, and healing rate wounds was normal. Histologically, however, found abnormalities organization wound epithelium....
The Nrf2 transcription factor is a key player in the cellular stress response through its regulation of cytoprotective genes. In this study we determined role Nrf2-mediated gene expression keratinocytes for skin development, wound repair, and carcinogenesis. To overcome compensation by related Nrf1 Nrf3 proteins, expressed dominant-negative mutant (dnNrf2) epidermis transgenic mice. functionality transgene product was verified vivo using mice doubly dnNrf2 an Nrf2-responsive reporter gene....
Peroxiredoxin 6 (Prdx6) is a cytoprotective enzyme with largely unknown in vivo functions. Here, we use Prdx6 knockout mice to determine its role UV protection and wound healing. UV-mediated keratinocyte apoptosis enhanced Prdx6-deficient mice. Upon skin injury, observe severe hemorrhage the granulation tissue of animals, which correlates extent oxidative stress. At ultrastructural level endothelial cells appear highly damaged, their rate enhanced. Knock-down cultured also increases...
The primary aim of this report is to assist scientists in selecting more reliable/suitable identification (ID) methods for their studies. This especially true genetically altered (GA) animals where individual strictly necessary link samples, research design and genotype. Federation European Laboratory Animal Science Associations working group was provide an update the used identify rodents different situations assess implications animal welfare. ID procedures are indispensable prerequisite...
To identify key regulators of cutaneous wound repair, we analyzed a subtractive cDNA library normal and wounded mouse skin. One the identified genes encodes chemokine receptor CCR1, which binds several chemokines present at site. Expression CCR1 was barely detectable in nonwounded skin, but strong up‐regulation observed after injury to wild‐type mice. Most important, healing abnormalities glucocorticoid‐treated mice activin‐overexpressing transgenic correlated with an altered expression...
An increasing number of patients are being treated with growth hormone (GH) for the enhancement body but also as an anti-aging strategy. However, side effects GH have been poorly defined. In this study we determined effect on wound repair and its mechanisms action at site. For purpose, performed healing studies in transgenic mice overexpressing GH. Full thickness incisional excisional wounds animals developed extensive, highly vascularized granulation tissue. bursting strength was not...
Colony management of gene-modified animals is time-consuming, costly and affected by random events related to Mendelian genetics, fertility litter size. Careful planning mandatory ensure successful outcomes using the least number animals, hence adhering 3R principles animal welfare. Here we have developed an R package, accessible also through interactive public website, that optimizes breeding design providing information about optimal breedings needed obtain defined outcomes, taking into...
Abstract Animal breeding is time-consuming, costly, and affected by stochastic events related to Mendelian genetics, fertility, litter size. Careful planning mandatory ensure a successful outcome using the least number of animals, hence adhering 3Rs animal welfare. We have developed an R package, accessible also through interactive website, that optimizes design provides comprehensive report suitable for any breeder genetically defined traits.
Abstract Animal breeding is time-consuming, costly, and affected by stochastic events related to Mendelian genetics, fertility, litter size. Careful planning mandatory ensure a successful outcome using the least number of animals, hence adhering 3Rs animal welfare. We have developed an R package, accessible also through interactive website, that optimizes design provides comprehensive report suitable for any breeder genetically defined traits.