A5073418458- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Asthma and respiratory diseases
- Eosinophilic Esophagitis
- Respiratory viral infections research
- Neonatal Respiratory Health Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Exercise and Physiological Responses
- Immune Response and Inflammation
- Immune cells in cancer
- Air Quality and Health Impacts
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Advanced Glycation End Products research
- Stress Responses and Cortisol
- Pediatric health and respiratory diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Adhesion Molecules Research
- Cancer-related gene regulation
- Heme Oxygenase-1 and Carbon Monoxide
- High Altitude and Hypoxia
- Fibroblast Growth Factor Research
- Smoking Behavior and Cessation
- Influenza Virus Research Studies
- Adipose Tissue and Metabolism
University of Utah
2019-2025
Pulmonary Associates
2024
George E. Wahlen Department of VA Medical Center
2021-2024
University of Utah Health Care
2024
Lake City VA Medical Center
2022-2024
VA Salt Lake City Healthcare System
2021-2022
Veterans Health Administration
2020-2021
University of Nebraska Medical Center
2016-2019
Nebraska Medical Center
2017-2018
University of Nebraska at Omaha
2017
Lymph nodes (LNs) are integral sites for the generation of immune tolerance, migration CD4⁺ T cells, and induction Tregs. Despite importance LNs in regulation inflammatory responses, LN-specific factors that regulate cell precise LN structural domains which differentiation occurs remain undefined. Using intravital fluorescent microscopy, we found alloreactive cells traffic distinctly into tolerant colocalize exclusive regions with alloantigen-presenting a process required Treg induction....
Obesity has been associated with greater severity of influenza virus infection and impaired host defense. Exercise may confer health benefits even when weight loss is not achieved, but it determined if regular exercise improves immune defense against A (IAV) in the obese condition. In this study, diet-induced mice lean control exercised for eight weeks followed by viral infection. reduced disease both non-obese mice, mechanisms differed. reversed obesity-associated delay...
The airway epithelium is a broad interface with the environment, mandating well-orchestrated responses to properly modulate inflammation. Classically, autophagy homeostatic pathway triggered in response external cellular stresses, and elevated chronic diseases. Recent findings highlight additional role of vesicle trafficking protein secretion, implicating pathways complex disease. Th2 cytokines, IL-13 IL-4, are increased asthma other diseases contributing Previously, we observed that...
Agriculture exposures are associated with reducing the risk of allergy and asthma in early life; yet, repeated later life chronic bronchitis obstructive pulmonary diseases. The objective this study was to investigate airway inflammatory response organic dust extract (ODE) mice established ovalbumin (OVA)-induced experimental asthma. C57BL/6 were either OVA sensitized/aerosol-exposed or saline (Sal) sensitized/aerosol-challenged. Both groups then subsequently challenged once intranasal swine...
Here, we examine how prenatal inflammation shapes tissue function and immunity in the lung by reprogramming tissue-resident immune cells from early development. Maternal, but not fetal, type I interferon-mediated provokes expansion hyperactivation of group 2 innate lymphoid (ILC2s) seeding developing lung. Hyperactivated ILC2s produce increased IL-5 IL-13 are associated with acute Th2 bias, decreased Tregs, persistent eosinophilia into adulthood. ILC2 is recapitulated adoptive transfer fetal...
Respiratory viral infections are one of the leading causes need for emergency care and hospitalizations in asthmatic individuals, airway-secreted cytokines released within hours infection to initiate these exacerbations. IL-33, specifically, contributes allergic exacerbations by amplifying type 2 inflammation. We hypothesized that blocking IL-33 RSV-induced exacerbation would significantly reduce Sensitized BALB/c mice were challenged with aerosolized ovalbumin (OVA) establish inflammation,...
Asthma is a chronic airway condition that occurs more often in women than men during reproductive years. Population studies have collectively shown long-term use of oral contraceptives decreased the onset asthma age. In current study, we hypothesized steady-state levels estrogen would reduce inflammation and hyperresponsiveness to methacholine challenge.Ovariectomized BALB/c mice (Ovx) were implanted with subcutaneous hormone pellets (estrogen, OVX-E2) deliver consistent [68 ± 2 pg/mL], or...
Lung M2 macrophages are regulators of airway inflammation, associated with poor lung function in allergic asthma. Previously, we demonstrated that IL-4-induced gene expression correlated tyrosine phosphorylation the insulin receptor substrate-2 (IRS-2) macrophages. We hypothesized negative regulation IRS-2 activity after IL-4 stimulation is dependent upon serine IRS-2. Herein, describe an inverse relationship between (Tyr(P)) and (Ser(P)) stimulation. Inhibiting phosphatase increased...
Type 2 immunity is initiated through a synergistic response between innate and adaptive immune cells to facilitate host-pathogen defense wound repair, yet aberrant responses can contribute chronic inflammation allergic disease. CD4+ type helper T (Th2) the secretion of cytokines such as IL-4, IL-5, IL-13. While Th2 program governed by transcription factor GATA3, less known about regulators that fine-tune cytokine response. We used proximity labeling system map proteins associated with...
Agriculture organic dust exposures induce lung disease with lymphoid aggregates comprised of both T and B cells. The precise role cells in mediating inflammation is unknown, yet might be relevant given the emerging obstructive pulmonary associated autoimmunity. Using an established animal model, C57BL/6 wild-type (WT) B-cell receptor (BCR) knock-out (KO) mice were repetitively treated intranasal inhalation swine confinement extract (ODE) daily for 3 weeks lavage fluid, tissues, serum...
Skeletal health consequences associated with inflammatory diseases of the airways significantly contribute to morbidity. Sex differences have been described independently for lung and bone diseases. Repetitive inhalant exposure lipopolysaccharide (LPS) induces loss deterioration in male mice, but comparison effects females are unknown. Using an intranasal inhalation model, 8-week-old C57BL/6 female mice were treated daily LPS (100 ng) or saline 3 weeks. Bronchoalveolar lavage fluids,...
Trafficking and differentiation of naive CD4+ regulatory T cells (Treg) within the lymph node (LN) are integral for tolerance induction. The LN is comprised stromal fibers that dictate lymphocyte migration structure, organization, microanatomic domains. Distribution fiber ER-TR7 changes after antigenic challenge, but contributions to resulting immune response remain undefined. We hypothesized these structural affect cell fate subsequently allograft survival.C57BL/6 mice were left (untreated)...
COVID-19 causes severe disease with poor outcomes. We tested the hypothesis that early SARS-CoV-2 viral infection disrupts innate immune responses. These changes may be important for understanding subsequent clinical obtained residual nasopharyngeal swab samples from individuals who requested testing symptoms at drive-through sites operated by University of Utah. applied multiplex immunoassays, real-time polymerase chain reaction assays and quantitative proteomics to 20 virus-positive...
Journal Article Kinetics of UV254 inactivation selected viral pathogens in a static system Get access T. Cutler, Cutler Department Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA Search for other works by this author on: Oxford Academic Google Scholar C. Wang, Wang USADepartment Statistics, Q. Qin, Qin Microbiology Preventive F. Zhou, Zhou K. Warren, Warren K.‐J. Yoon, Yoon S.J. Hoff, Hoff Agriculture Biosystems Engineering, J. Ridpath, Ridpath...
Inhalation of organic dusts in agricultural environments causes airway inflammatory diseases. Despite advances understanding the response to dust-induced inflammation, less is known about transition from lung injury repair and recovery. The objective this study was define post-inflammation homeostasis events following injury. Using an established protocol, mice were intranasally treated with swine confinement facility dust extract (ODE) daily for 3 weeks (repetitive exposure) or ODE followed...
Asthmatic women tend to develop severe airway disease in their reproductive years, and 30%–40% of asthmatic have peri-menstrual worsening asthma symptoms. This indicates that fluctuations ovarian hormones are involved advancement exacerbation Group 2 innate lymphoid cells, or ILC2, readily detected allergic conditions, such as rhinosinusitis, individuals nasal polyps do allergen exposures, asthma. ILC2 localized immune cells activated by IL-33, an cytokine perpetuates inflammation driving...
Abstract Exposure to e-cigarette vapors alters important biologic processes including phagocytosis, lipid metabolism, and cytokine activity in the airways alveolar spaces. Little is known about mechanisms underpinning conversion e-cigarette, or vaping, product use-associated lung injury (EVALI) from normal use otherwise healthy individuals. We compared cell populations inflammatory immune bronchoalveolar lavage fluid individuals with EVALI users without respiratory disease controls found...
SARS-CoV-2 infection has claimed just over 1.1 million lives in the US since 2020. Globally, respiratory spread to 771 people and caused mortality 6.9 individuals date. Much of early literature showed that immunity was defective stages pandemic, leading heightened and, sometimes, chronic inflammatory responses lungs. This lung-associated ‘cytokine storm’ or release syndrome’ led need for oxygen supplementation, distress syndrome, mechanical ventilation a relatively high number people. In...
Perimenstrual worsening of asthma occurs in up to 40% women with asthma, leading increased acute exacerbations requiring clinical care. The role sex hormones during these times remains unclear. In the current study, we used a translational approach determine whether progesterone exacerbates allergic inflammation traditional chicken egg ovalbumin (OVA) model BALB/c mice. Simultaneously, peripheral blood mononuclear cells (PBMC) from healthy human donors assess effects on circulating group 2...