A5103224963- Diabetes and associated disorders
- Diet, Metabolism, and Disease
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Microbial Inactivation Methods
- IL-33, ST2, and ILC Pathways
- Plant tissue culture and regeneration
- Liver Disease and Transplantation
- Diabetes Management and Research
- RNA Research and Splicing
- Gut microbiota and health
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Advanced biosensing and bioanalysis techniques
- Single-cell and spatial transcriptomics
- Dietary Effects on Health
- Cellular Mechanics and Interactions
- Endoplasmic Reticulum Stress and Disease
- Neuroendocrine regulation and behavior
- Gastrointestinal motility and disorders
- Advanced Biosensing Techniques and Applications
Instituto de Agroquímica y Tecnología de Alimentos
2023-2025
Consejo Superior de Investigaciones Científicas
1994-2025
Centro de Investigacion Principe Felipe
2020-2022
Institute for Integrative Systems Biology
2017
Bio-Rad (United States)
2006-2008
Zero to Three
2006
University of California, Berkeley
1999
Universidad Autónoma de Madrid
1994
Centro de Biología Molecular Severo Ochoa
1994
The animal microbiota (including the human microbiota) plays an important role in keeping physiological status of host healthy. Research seeks greater insight into whether changes composition and function are associated with disease. We analyzed published 16S rRNA shotgun metagenomic sequencing (SMS) data pertaining to gut microbiotas 99 subjects monitored over time. Temporal fluctuations microbial revealed significant differences due factors such as dietary changes, antibiotic intake, age,...
Nicotiana benthamiana plants transformed with the coat protein gene of tomato bushy stunt virus (TBSV) failed to elicit effective resistance when inoculated wild-type virus. Subsequently, R 1 and 2 progeny from 13 transgenic lines were a TBSV mutant containing defective gene. Mild symptoms typical those elicited in nontransformed infected initially appeared. However, within 4 weeks, up 20% sporadically began develop lethal syndrome characteristic infections. RNA hybridization immunoblot...
The Environmental Determinants of Diabetes in the Young (TEDDY) is a prospective birth cohort designed to study type 1 diabetes (T1D) by following children with high genetic risk. An integrative multi-omics approach was used evaluate islet autoimmunity etiology, identify disease biomarkers, and understand progression over time.
B and T cell receptor (immune) repertoires can represent an individual's immune history. While current repertoire analysis methods aim to discriminate between health disease states, they are typically based on only a limited number of parameters. Here, we introduce immuneREF: quantitative multidimensional measure adaptive (and transcriptome) similarity that allows interpretation variation by relying both features cross-referencing simulated experimental datasets. To quantify landscapes...
Abstract Recent evidence suggests a role of sensory neurons expressing the sodium channel Nav1.8 on energy homeostasis control. Using murine diphtheria toxin ablation strategy and ad libitum time-restricted feeding regimens control or high-fat high-sugar diets, here we further explore function these food intake regulation gastrointestinal elements transmitting immune nutrient sensing. The Nav1.8+ neuron increases in feeding, exacerbates daily body weight variations. Mice lacking show...
Hypercaloric diets overactivate the intestinal immune system and disrupt microbiome epithelial cell functions, impairing glucose metabolism. The origins of this inflammatory cascade are poorly characterized. We investigated involvement proinflammatory group 1 innate lymphoid cells (ILC1s) in obesity progression metabolic disruption. In obese mice, we studied longitudinally ILC1s response to diet depletion address its role obesity. required for expansion pro-inflammatory macrophages ILC2s....
Abstract The gut microbiota may protect against obesity and chronic metabolic conditions by regulating the immune response to dietary triggers. Yet specific bacteria that control overactivation of system in their mode action remain largely unknown. Here we surveyed 7,569 human metagenomes observed an association between symbiont Phascolarctobacterium faecium non-obese adults regardless nationality, sex or age. In a mouse model diet-induced obesity, confirmed specificity P. DSM 32890...
Periodontal diseases are highly prevalent in dogs and intricately interconnected with the composition functional attributes of oral microbiota. The demand for non-invasive interventions to support health presents an opportunity ingredients. novel postbiotic heat-treated (HT) Lactiplantibacillus plantarum CECT 9161 inhibited growth biofilm formation microorganisms vitro. vitro saliva-derived biofilms was also revealed microbiome modulation. Two doses (LOW: 5 mg dog/day, HIGH: 25 mg/dog/day)...
Genomic DNA clones containing the T-cell-specific human MAL gene were isolated. Restriction and sequence analysis revealed four exons three introns. Each hydrophobic segment of together with its adjacent hydrophilic correlates closely one exon gene. RNase protection that previously described mRNA, which contains sequences present in exons, is mRNA species predominant T-cells. A remarkable similarity was found between hydrophobicity pattern those peripheral membrane protein 22 (PMP-22) 16-kDa...
ABSTRACT We have designed a DNA cassette to transcribe defective interfering (DI) RNAs of tomato bushy stunt virus (TBSV) and investigated their potential protect transgenic Nicotiana benthamiana plants from tombusvirus infections. To produce with authentic 5′ 3′ termini identical those the native B10 DI RNA, RNA sequences were flanked by ribozymes (RzDI). When RzDI transcribed in vitro mixed parental TBSV transcripts inoculated into protoplasts or plants, they became amplified, reduced...
Abstract Patients with liver cirrhosis may develop minimal hepatic encephalopathy (MHE) which affects their quality of life and span. It has been proposed that a shift in peripheral inflammation triggers the appearance MHE. However, mechanisms involved this immune system remain unknown. In work we studied broad molecular changes induction MHE goal identifying (1) altered genes pathways blood cells associated to MHE, (2) serum metabolites cytokines modified levels patients (3) MHE-regulated...
T-cell receptor (TCR) analysis is relevant for the study of immune system diseases. The expression TCRs usually measured with targeted sequencing approaches where TCR genes are selectively amplified. However, many non-targeted RNA-seq experiments also contain reads genes, which could be leveraged while reducing sample requirements and costs. Moreover, a step-by-step pipeline processing transcriptome to deliver repertoire data missing, these types analyses not included in studies...
Minimal hepatic encephalopathy (MHE) is associated with changes in the immune system including an increased pro-inflammatory environment and altered differentiation of CD4+ T lymphocytes. The mechanisms remain unknown. Changes extracellular vesicle (EV) cargo proteins miRNAs could play a main role as mediators MHE. aim was to assess whether plasma EVs from MHE patients played inducing lymphocyte subtypes patients. We characterized miRNA protein 50 cirrhotic (27 without 23 MHE) 24 controls....
We have characterized the three cis elements responsible for promoter strength present in 5′-flanking proximal region of MAL, a human T-cell-specific gene encoding proteolipid protein detergent-insoluble complexes high molecular weight. The first element consisted an initiator sequence that, curiously, was reverse orientation compared to that standard elements. other two were contained 126 bp upstream mRNA initiation site, and tandem array one GC box GA box. corresponds consensus site...
Abstract Actin‐binding proteins regulate the dynamic structure and function of actin filaments in cell. Much is known about how manipulation actin‐binding affects filaments; however, little cell proteins. We addressed this question by utilizing two technologies: RNA interference 2‐dimensional gel electrophoresis. knocked down β‐actin expression HeLa cells using short interfering applied 2‐DGE to examine alterations proteome. revealed a 2–5 fold increases four protein spots on 2‐D gels...
Abstract B- and T-cell receptor (immune) repertoires can represent an individual’s immune history. While current repertoire analysis methods aim to discriminate between health disease states, they are typically based on only a limited number of parameters (e.g., clonal diversity, germline usage). Here, we introduce immuneREF: quantitative multi-dimensional measure adaptive (and transcriptome) similarity that allows interpretation variation by relying both features cross-referencing simulated...
Electroporation is a valuable tool for nucleic acid delivery because it can be used wide variety of cell types. Many scientists are shifting towards the use types increasing scientific interest, including lineage‐specific types, bone marrow cells and stem cells, but these difficult to transfect. The ability transfect with molecules interest at relatively high efficiency while maintaining viability would offer new elucidation pathway(s) in which gene product involved. In this poster, we...
Modulating gene expression is essential to achieve a better understanding of function. The transfer exogenous nucleic acids such as plasmids or small interfering (si)RNAs into mammalian cells an important technique for the study and analyses function, expression, regulation, mutation, it has advanced basic cellular research, enabled drug target identification, validation. Electroporation well‐established method, effective means transferring cells. shift today's research more scientifically...