A5101506380- Cancer Cells and Metastasis
- Respiratory viral infections research
- Inhalation and Respiratory Drug Delivery
- Neonatal Respiratory Health Research
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Plant Disease Resistance and Genetics
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Influenza Virus Research Studies
- Medical Research and Treatments
- Infection Control and Ventilation
- Plant Stress Responses and Tolerance
- Ferroptosis and cancer prognosis
- Plant Pathogenic Bacteria Studies
- Microfluidic and Bio-sensing Technologies
- Cytomegalovirus and herpesvirus research
- Ethics in Clinical Research
- Fire effects on ecosystems
- Neurological Disease Mechanisms and Treatments
- Plant-Microbe Interactions and Immunity
- Thyroid Cancer Diagnosis and Treatment
- History of Medical Practice
- Silicon Effects in Agriculture
University of Hong Kong
2019-2025
Guangxi University
2019-2025
Chinese University of Hong Kong
2022-2025
Hong Kong Science and Technology Parks Corporation
2022-2024
First Affiliated Hospital of Harbin Medical University
2020-2022
Harbin Medical University
2020-2022
Sichuan Academy of Agricultural Sciences
2022
Zhejiang Chinese Medical University
2021
Zhujiang Hospital
2021
Southern Medical University
2021
Viruses are obligate intracellular microbes that exploit the host metabolic machineries to meet their biosynthetic demands, making these pathways potential therapeutic targets. Here, by exploring a lipid library, we show AM580, retinoid derivative and RAR-α agonist, is highly potent in interrupting life cycle of diverse viruses including Middle East respiratory syndrome coronavirus influenza A virus. Using click chemistry, overexpressed sterol regulatory element binding protein (SREBP) shown...
The high transmissibility of SARS-CoV-2 Omicron subvariants was generally ascribed to immune escape. It remained unclear whether the emerging variants have gradually acquired replicative fitness in human respiratory epithelial cells. We sought evaluate BA.5 and earlier physiologically active organoids. exhibited a dramatically increased capacity infectivity than B.1.1.529 an ancestral strain wildtype (WT) nasal airway spike pseudovirus showed significantly higher entry efficiency that...
The airways and alveoli of the human respiratory tract are lined by two distinct types epithelium, which primary targets viruses. We previously established long-term expanding lung epithelial organoids from tissues developed a 'proximal' differentiation protocol to generate mucociliary airway organoids. However, organoid system with bipotential alveolar remains elusive. Here we defined 'distal' approach same source for derivation consisting type I II cells (AT1 AT2, respectively)...
An in vitro model of the nasal epithelium is imperative for understanding cell biology and virus-host interaction human upper respiratory tract. Here we report an organoid culture system epithelium.
Host AP2M1 protein is exploited by diverse pathogenic viruses via their YxxØ motif during viral replication cycle.
The respiratory epithelium, particularly the airway is primary infection site for pathogens. apical surface of epithelial cells constantly exposed to external stimuli including invading Efforts have been made establish organoid cultures recapitulate human tract. However, a robust and simple model with an easily accessible would benefit research. Here, we report generation characterization apical-out organoids from long-term expandable lung that previously established. morphologically...
Horseshoe bats host numerous SARS-related coronaviruses without overt disease signs. Bat intestinal organoids, a unique model of bat epithelium, allow direct comparison with human organoids. We sought to unravel the cellular mechanism(s) underlying tolerance by comparing innate immunity in and optimized culture medium, which enabled consecutive passage organoids for over one year. Basal expression levels IFNs IFN-stimulated genes were higher than their counterparts. Notably, mounted more...
Rice, being the most important food crop in China, is also world's largest producer and consumer, accounting for 30% of global production consumption. In 2023, brown stripes were observed on leaf sheaths diseased rice seedlings a Jingxi field (22°51'N, 105°56'E) Guangxi, China. The investigated area fields, which was about 1.5 hectares, had an estimated disease incidence 15 ± 5%. Leaf samples exhibiting symptoms suspected stripe collected stored at 4 °C bacterial isolation. junction margins...
Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne bunyavirus that causes severe disease in humans case-fatality rates of up to 30%. There are currently very limited treatment options for SFTSV infection. We conducted a drug repurposing program by establishing two-tier test system rapidly screen Food and Drug Administration- (FDA)-approved library compounds anti-SFTSV activity vitro. identified five inhibited replication at low micromolar concentrations,...
The lack of a robust system to reproducibly propagate HRV-C, family viruses refractory cultivation in standard cell lines, has substantially hindered our understanding this common respiratory pathogen. We sought develop an organoid-based and characterize virus-host interaction using organoids. demonstrate that airway organoids sustain serial virus passage with the aid CYT387-mediated immunosuppression, whereas nasal more closely simulate upper achieve without any intervention. Nasal are...
The lack of a robust in vitro model the human respiratory epithelium hinders understanding biology and pathology system. We describe defined protocol to derive lung organoids from adult stem cells tissue induce proximal differentiation generate mature airway organoids. are then consecutively expanded for over 1 year with high stability, while differentiated used morphologically functionally simulate near-physiological level. Thus, we establish organoid epithelium. long-term expansion...
Background Thyroid carcinoma (THCA) has a low mortality rate, but its incidence been rising over the years. We need to pay attention progression and prognosis. In this study, transcriptome sequencing analysis bioinformatics methods were used screen key genes associated with THCA development analyse their clinical significance diagnostic value. Methods collected 10 pairs of tissues noncancerous tissues, these samples for identify disordered genes. The gene expression profiles obtained from...
A robust in vitro model of the human respiratory epithelium, including alveolar and airway is essential for understanding biology pathology system. We previously described a protocol to derive lung organoids from primary tissues. now describe induce bidirectional differentiation generate mature or organoids. The are consecutively expanded over one year with high stability, while differentiated morphologically functionally simulate epithelium near-physiological level. Thus, we establish...
Multiple PDZ domain protein (MPDZ) is a component of the crumbs cell polarity complex and ubiquitously expressed in mammalian tissues. In mouse sperm, MPDZ was identified as regulator sperm acrosome reaction past two decades. Here, relationship between motility discovered. During breeding, we found that MPDZ-null mice had smaller litter size. The Ca2+ signal decreased. Computer-assisted semen analysis revealed males reduced motility. humans, expression positively associated with Considering...
Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) can induce apoptosis by releasing reactive oxygen species (ROS) and nitrogen species, which cause mitochondrial damage. The present study aimed to investigate the protective effects of flavonoids from leaves Carya cathayensis Sarg. against H<sub>2</sub>O<sub>2</sub>‑induced oxidative damage in vitro. bioactivity total (TFs) five monomeric [cardamonin (Car), pinostrobin chalcone, wogonin, chrysin pinocembrin] <em>Carya cathayensis</em> (LCCS)...
Nowadays so many public fitness facilities in residential quarters are not suitable for the elderly who requires nursing care to use, but their demands of rehabilitation can't be neglected.In this paper, design proposal based on demand characteristic is presented according previous investigation ways living.The principle inclusive applied demonstrate feasibility by analysis practice, function positioning, easiness use and safety, which would provide references care.
Here, we report the genomic sequence and genetic variations of a Tomato yellow mottle-associated virus. The virus isolated from field tomato (Solanum lycopersicum) plant in Chengdu, southwestern China, was sequenced via both Illumina Sanger technologies. Phylogeny indicates that its genome is close to reported S. lycopersicum collected 2013 but far Solanum nigrum 2020.