A5011772702- Immune cells in cancer
- Epigenetics and DNA Methylation
- Retinoids in leukemia and cellular processes
- Antioxidant Activity and Oxidative Stress
- Retinal Development and Disorders
- Muscle Physiology and Disorders
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- Neonatal Respiratory Health Research
- Estrogen and related hormone effects
- GDF15 and Related Biomarkers
- Cancer-related gene regulation
- Nutrition and Health in Aging
Johns Hopkins All Children's Hospital
2020-2024
Johns Hopkins University
2020-2024
Johns Hopkins Medicine
2020
Muscle regeneration is the result of concerted action multiple cell types driven by temporarily controlled phenotype switches infiltrating monocyte-derived macrophages. Pro-inflammatory macrophages transition into a that drives tissue repair through production effectors such as growth factors. This orchestrated sequence regenerative inflammatory events, which we termed regeneration-promoting program (RPP), essential for proper repair. However, it not well understood how specialized...
Prior exposure to microenvironmental signals could fundamentally change the response of macrophages subsequent stimuli. It is believed that T helper-2 (Th2)-cell-type cytokine interleukin-4 (IL-4) and Toll-like receptor (TLR) ligand-activated transcriptional programs mutually antagonize each other, no remarkable convergence has been identified between them. In contrast, here, we show IL-4-polarized established a hyperinflammatory gene expression program upon lipopolysaccharide (LPS)...
Macrophages polarize into functionally distinct subtypes while responding to microenvironmental cues. The identity of proximal transcription factors (TFs) downstream from the polarization signals are known, but their activity is typically transient, failing explain long-term, stable epigenomic programs developed. Here, we mapped early and late changes interleukin-4 (IL-4)-induced alternative macrophage polarization. We identified TF, growth response 2 (EGR2), bridging transient gene...
Retinoid X receptors (RXRs) are nuclear transcription factors that partner with other to regulate numerous physiological processes. Although RXR represents a valid therapeutic target, only few RXR-specific ligands (rexinoids) have been identified, in part due the lack of clarity on how rexinoids selectively modulate response. Previously, we showed rexinoid UAB30 potentiates all-trans-retinoic acid (ATRA) signaling human keratinocytes, by stimulating ATRA biosynthesis. Here, examined...
Rexinoids are agonists of nuclear rexinoid X receptors (RXR) that heterodimerize with other to regulate gene transcription. A number selective RXR have been developed for clinical use but their application has hampered by the unwanted side effects associated rexinoids and a limited understanding mechanisms action across different cell types. Our previous studies showed treatment organotypic human epidermis low toxicity UAB30 UAB110 resulted in increased steady-state levels all- trans...
Abstract Classical monocytes are recruited to tumors and undergo transcriptional reprogramming resulting in tumor-promoting functions. Epigenomic features, such as post-translational modification of histones chromatin accessibility, key determinants transcription factor binding thereby play an important role determining responses the tissue environment. It is unknown what extent epigenetic landscape peripheral rewired by cancer how this could shape their response upon recruitment tumor...
Alveolar macrophages (AMs) act as gatekeepers of the lung's immune responses, serving essential roles in recognizing and eliminating pathogens. The transcription factor (TF) Early Growth Response 2 (EGR2) has been recently described required for mature AMs mice; however, its mechanisms action have not explored. Here, we identified EGR2 an epigenomic regulator likely direct proximal transcriptional activator using approaches (RNA-sequencing, ATAC-sequencing, CUT&RUN). predicted targets...