A5103194271- Genetic Neurodegenerative Diseases
- Liver Disease Diagnosis and Treatment
- Mitochondrial Function and Pathology
- Diet, Metabolism, and Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced MRI Techniques and Applications
- Neurological disorders and treatments
- Advanced Neuroimaging Techniques and Applications
- Ferroptosis and cancer prognosis
- CRISPR and Genetic Engineering
- Cerebrospinal fluid and hydrocephalus
- Genomics, phytochemicals, and oxidative stress
- Lanthanide and Transition Metal Complexes
- Advanced Photocatalysis Techniques
- Traditional Chinese Medicine Analysis
- Muscle Physiology and Disorders
- Liver Disease and Transplantation
- Immune Response and Inflammation
- Cancer, Lipids, and Metabolism
- Cynara cardunculus studies
- Adipose Tissue and Metabolism
- Phytochemical Studies and Bioactivities
- Ga2O3 and related materials
- Multiple Sclerosis Research Studies
- Traumatic Brain Injury Research
Shandong University of Traditional Chinese Medicine
2022-2025
Johns Hopkins Medicine
2020-2025
Johns Hopkins University
2020-2025
Shandong University
2018-2019
State Key Laboratory of Crystal Materials
2018-2019
Shanghai University of Traditional Chinese Medicine
2013-2017
Shanghai University
2014
Abstract Huntington’s disease (HD) is a fatal, dominantly inherited neurodegenerative disorder caused by CAG trinucleotide expansion in exon 1 of the huntingtin ( HTT ) gene. Since reduction pathogenic mutant messenger RNA therapeutic, we developed allele-sensitive EX RNA-targeting CRISPR–Cas13d system (Cas13d–CAG that eliminates toxic fibroblasts derived from patients with HD and induced pluripotent stem cell-derived neurons. We show intrastriatal delivery Cas13d–CAG via an adeno-associated...
Multiple sclerosis (MS) is a chronic autoimmune neuroinflammatory disease found mostly in young adults the western world. Oxidative stress induced neuronal apoptosis plays an important role pathogenesis of MS. In current study, astragaloside IV (ASI), natural saponin molecule isolated from Astragalus membranceus, given at 20 mg/kg daily attenuated severity experimental encephalomyelitis (EAE) mice significantly. Further studies disclosed that ASI treatment inhibited increase ROS and...
Blood-brain barrier (BBB) plays a critical role in protecting the brain from toxins and pathogens. However, vivo tools to assess BBB permeability are scarce often require use of exogenous contrast agents. In this study, we aimed develop non-contrast arterial-spin-labeling (ASL) based MRI technique estimate water mice. By determining relative fraction labeled spins that were exchanged into tissue as opposed those remained cerebral veins, estimated indices global including extraction (E)...
Inhibition of microglia activation may provide therapeutic treatment for many neurodegenerative diseases. Astragaloside IV (ASI) with anti-inflammatory properties has been tested as a drug in clinical trials China. However, the mechanism ASI inhibiting neuroinflammation is unknown. In this study, we showed that inhibited both vivo and vitro. It could enhance glucocorticoid receptor (GR)-luciferase activity facilitate GR nuclear translocation microglial cells. Molecular docking TR-FRET...
The accumulation of mutant huntingtin protein aggregates in neurons is a pathological hallmark Huntington's disease (HD). glymphatic system, brain-wide perivascular network, facilitates the exchange interstitial fluid (ISF) and cerebrospinal (CSF), supporting solute clearance brain wastes. In this study, we employed dynamic glucose-enhanced (DGE) MRI to measure D-glucose from CSF as tool predict function mouse model HD. We found significantly diminished efficiency HD mice prior phenotypic...
Abstract Huntington’s disease is a dominantly inherited, fatal neurodegenerative disorder caused by CAG expansion in the huntingtin (HTT) gene, coding for pathological mutant HTT protein (mHTT). Because of its gain-of-function mechanism and monogenic aetiology, strategies to lower are being actively investigated as disease-modifying therapies. Most approaches currently targeted at manifest stage, where clinical outcomes used evaluate effectiveness therapy. However, almost 50% striatal volume...
Huntington's disease (HD) is a progressive neurodegenerative disorder caused by CAG-repeat expansion in exon-1 of the huntingtin gene. Currently, no disease-modifying therapies are available, with significant challenge evaluating therapeutic efficacy before clinical symptoms emerge. This highlights need for early biomarkers and intervention strategies. Therefore, it essential to develop characterize accurate mouse models identify preclinical development. In this study, we characterized...
Abstract Aim This study aimed to investigate the mechanisms through which diosgenin inhibits pathogenesis of non‐alcoholic fatty liver disease, focusing particularly on ferroptosis‐related pathways and its reliance nuclear factor erythroid 2‐related 2 . Materials Methods Using a rat model, we showed diosgenin's efficacy in reducing lipid deposition throughout body examined impact gene expression vivo Moreover, vitro experiments using human hepatocellular carcinoma cell line cells were...
Radix Astragali is famous for its beneficial effect on inflammation associated diseases. This study was to assess the efficacy of astragalosides (AST) extracted from Astragali, progression experimental autoimmune encephalomyelitis (EAE), and explore possible underlying molecular mechanisms. EAE induced by subcutaneous immunization MOG35–55. Infiltration inflammatory cells examined HE staining. ROS level detected measuring infiltrated hydroethidine. Leakage blood brain barrier (BBB) assessed...
In this work, we fabricated four different Ga<sub>2</sub>O<sub>3</sub> polymorphs, namely, α-, β-, γ-, δ-Ga<sub>2</sub>O<sub>3,</sub> and investigated their photocatalytic activities by the degradation of ethylene under ultraviolet (UV) light irradiation.
Huntington's disease (HD) is an autosomal dominant neurodegenerative caused by a single mutation in the huntingtin gene (HTT). Normal HTT has CAG trinucleotide repeat at its N-terminal within range of 36. However, once repeats exceed 37, mutant (mHTT) will encode protein (mHTT), which results neurodegeneration brain, specifically striatum and other brain regions. Since was discovered, there have been many research efforts to understand mechanism develop therapeutic strategies treat HD. large...
Abstract Emerging cellular and molecular studies are providing compelling evidence that altered brain development contributes to the pathogenesis of Huntington’s disease (HD). There has been lacking longitudinal system-level data obtained from in vivo HD models supporting this hypothesis. Our human MRI study children adolescents with indicates striatal differs between control groups, initial hypertrophy more rapid volume decline group. In study, we aimed determine whether recapitulates...
ABSTRACT Huntington’s disease (HD) is a neurodegenerative disorder that presents with progressive motor, mental, and cognitive impairment leading to early disability mortality. The accumulation of mutant huntingtin protein aggregates in neurons pathological hallmark HD. glymphatic system, brain-wide perivascular network, facilitates the exchange interstitial fluid (ISF) cerebrospinal (CSF), supporting solute clearance including abnormal proteins from mammalian brains. In this study, we...
Objective Some previous studies have suggested a potential link between stroke and gastroesophageal reflux disease (GERD). We used two-sample bidirectional Mendelian randomization (MR) method to explore the causal relationship GERD. Design Summary-level data derived from published genome-wide association (GWAS) were employed for analyses. Single-nucleotide polymorphisms (SNPs) as instrumental variables (IVs) ( n = 446,696) its common subtypes ischemic (IS) 440,328), large vessel (LVS)...
A non-invasive magnetization transfer indirect spin labeling (MISL) MRI method is developed to quantify the water exchange between cerebrospinal fluid (CSF) and other tissues in brain examine age-dependence of exchange.
The excessive activation of microglia in many neurodegenerative diseases is detrimental to neuronal survival. Isoastragaloside I (ISO I) a natural saponin molecule found within the roots Astragalus membranaceus, famous traditional Chinese medicine. In present study, anti‑inflammatory effects and mechanisms action ISO on activated BV-2 cells stimulated with lipopolysaccharide (LPS) were investigated. dose‑dependently inhibited release nitric oxide (NO) tumor necrosis factor (TNF)-α...
Nemo-like kinase (NLK), an evolutionarily conserved serine/threonine kinase, is highly expressed in the brain, but its function adult brain remains not well understood. In this study, we identify NLK as interactor of huntingtin protein (HTT). We report that levels are significantly decreased HD human and models. Importantly, overexpression striatum attenuates atrophy, preserves striatal DARPP32 reduces mutant HTT (mHTT) aggregation mice. contrast, genetic reduction exacerbates atrophy loss...