A5050537449- Peripheral Artery Disease Management
- Aortic Disease and Treatment Approaches
- Aortic aneurysm repair treatments
- Venous Thromboembolism Diagnosis and Management
- Cardiac Ischemia and Reperfusion
- Angiogenesis and VEGF in Cancer
- Immune cells in cancer
- Vascular Procedures and Complications
- Anesthesia and Neurotoxicity Research
- Cardiac, Anesthesia and Surgical Outcomes
- Anatomy and Medical Technology
- Congenital Heart Disease Studies
- Extracellular vesicles in disease
- Organ Transplantation Techniques and Outcomes
- Acute Ischemic Stroke Management
- Cerebrovascular and Carotid Artery Diseases
- Surgical Simulation and Training
- Electrospun Nanofibers in Biomedical Applications
- Cardiac and Coronary Surgery Techniques
- Renal and Vascular Pathologies
- Traumatic Brain Injury and Neurovascular Disturbances
- Blood transfusion and management
- Blood donation and transfusion practices
- Mesenchymal stem cell research
- Abdominal vascular conditions and treatments
University of Lübeck
2015-2025
University Hospital Schleswig-Holstein
2015-2025
Kiel University
2017-2022
Universität Hamburg
2009
University of British Columbia
1973
1. This report defines the indications, and describes in detail a technique for atlanto-axial arthrodesis. Open reduction, with wire fixation bone grafting, achieves objective of immediate stabilisation an unstable C. 1-2 articulation. 2. The method is illustrated by fifteen consecutive patients who had fourteen whom excellent results. 3. When indications are correct, arthrodesis described safe effective procedure having success rate.
BackgroundPatient Blood Management (PBM) is a patient-centred, systematic, evidence-based approach to improve patient outcomes by managing and preserving patient's own blood whilst promoting safety empowerment. The effectiveness of PBM over longer period have not yet been investigated.MethodsWe performed prospectively designed, multicentre follow-up study with non-inferiority design. Data were retrospectively extracted case-based from electronic hospital information systems. All in-hospital...
Abstract The MMI Symani ® is a recently approved robotic microsurgical system for surgical procedures in adults. enables the surgeon to create microanastomoses. Clinical applications so far include lymphatic vessels surgery and creation of special flap plastics. use coronary arteries has not yet been assessed. aim this preclinical study was evaluate applicability anastomoses cadaveric porcine model. A total 12 were performed by three senior cardiovascular surgeons on left main artery hearts....
Remote ischemic preconditioning (RIPC) is a phenomenon, whereby repeated, non-lethal episodes of ischemia to an organ or limb exert protection against ischemia–reperfusion (I/R) injury in distant organs. Despite intensive research, there still apparent lack knowledge concerning the RIPC-mediated mechanisms, especially intestine. Aim this study was evaluate possible protective effects RIPC on intestinal I/R injury. Thirty rats were randomly assigned four groups: I/R; + RIPC; Sham; Sham RIPC....
Aneurysms of an aberrant right subclavian artery (ARSA) are rare but constitute a potentially lethal condition, especially with concomitant Marfan syndrome (MFS). A 27-year-old female confirmed MFS presented relevant progression known aneurysm ARSA in MRI. The patient had undergone valve-sparing aortic root replacement (David procedure) 4 months prior. After interdisciplinary discussion, she underwent endovascular exclusion the using combination established vascular plugs and novel shape...
The Symani Surgical System is a novel robotic microsurgical platform. This study investigated its feasibility for arterial reconstructive techniques in an vitro model. Two senior surgeons without preliminary expertise surgery performed different porcine artery circulation model (bypass, patch plastic, and direct closure of arteriotomy). There was overall improvement procedure times with minor leakage rate upper range values the qualitative assessment anastomoses. In this model, seems to be...
Abstract A novel developed 4D bioprinting technique is used for the manufacturing of human‐scale, small‐diameter vascular grafts. Accordingly, a bio‐ink synthesized from hybrid molecule containing sodium alginate (SA) and collagen peptide (COP). Endothelial progenitor cells (EPC) isolated human whole blood are integrated into bioartificial graft as an autologous cell source. Likewise, umbilical vein endothelial (HUVEC) experimental standard. The evolving grafts printed by customized...
Abstract Remote ischemic preconditioning (RIPC) protects the heart against myocardial ischemia/reperfusion (I/R) injury and recent work also suggested chronic remote conditioning (cRIPC) for cardiovascular protection. Based on current knowledge that systemic immunomodulatory effects of RIPC anti-inflammatory capacity monocytes might be involved in protection, aim our study was to evaluate whether RIPC/cRIPC blood plasma is able induce in-vitro angiogenesis, identify responsible factors cell...
Abstract Background Large extracellular vesicles (L-EV) with a diameter between 1 and 10 µm are released by various cell types. L-EV contain transport active molecules which crucially involved in to communication. We have shown that secretory products of human regulatory macrophages (Mreg) bear pro-angiogenic potential in-vitro our recent findings show Mreg cultures also numerous large vesicular structures similar so far unknown characteristics function. Aim this study To characterize the...
Abstract Macrophages belong to the innate immune system, and we have recently shown that in vitro differentiated human regulatory macrophages (Mreg) release large extracellular vesicles (L-EV Mreg ) with an average size of 7.5 μm which regulate wound healing angiogenesis vitro. The aim this study was investigate whether L-EV also affect CD3/CD28-mediated activation T-cells. were using blood monocytes isolated from culture supernatants by differential centrifugation. Activation T-cells...
Employing growth factor-induced partial reprogramming in vitro, peripheral human blood monocytes can acquire a state of plasticity along with expression various markers pluripotency. These so-called programmable cells monocytic origin (PCMO) hold great promise regenerative therapies. The aim this translational study was to explore and exploit the functional properties PCMO for allogeneic cell transplantation therapy critical limb ischemia (CLI). Using our previously described differentiation...
Ischemia/reperfusion- (I/R-) induced organ damage represents one of the main causes death worldwide, and new strategies to reduce I/R injury are urgently needed. We have shown that programmable cells monocytic origin (PCMO) respond with release angiogenic mediators transplantation PCMO results in increased neovascularization. Human regulatory macrophages (Mreg), which also origin, been successfully employed clinical studies due their immunomodulatory properties. Here, we investigated whether...
Abstract Objectives The sequence of initial tissue ischaemia and consecutive blood flow restoration leads to ischaemia/reperfusion (I/R) injury, which is typically characterized by a specific inflammatory response. Migrating monocytes seem mediate the immune response in ischaemic tissues influence detrimental as well regenerative effects during I/R injury. Materials Methods To clarify role classical isolated human were subjected vitro (3 hours followed 24 reperfusion). Cellular resilience,...
Background: TAVI indications expand not only to low-risk patients but also with a more complex anatomy and comorbidities. Transfemoral retrograde access is recognized as the first preferred approach according current guidelines. However, this suitable in up 10–15% of patients, for whom an alternative non-femoral route required. Case Presentation: An 83-year-old male patient known aortic isthmus stenosis presented severe symptomatic stenosis. Computed tomography revealed subtotal stenosis,...
Abstract Background Macrophages are involved in tissue homeostasis, angiogenesis and immunomodulation. Proangiogenic anti-inflammatory macrophages (regulatory macrophages, Mreg) can be differentiated in-vitro from CD14 + monocytes by using a defined cell culture medium stimulus of IFNγ. Aim the study To scrutinize potential impact temporal IFNγ exposure on macrophage differentiation as such may lead to emergence distinct novel subtype. Methods Differentiation human Mreg was performed GMP...
Vivostat Platelet-Rich Fibrin® (PRF) is an autologous platelet concentrate used for the local treatment of chronic or complicated wounds. Still, its application this indication not evidence-based. Therefore, we performed monocentric retrospective pilot study investigating clinical outcome a wounds in 35 patients (23 male, 12 female, mean age 68.7 years) treated with PRF®. This population largest among published studies analyzing efficacy PRF® on so far. Using perpendicular method divided...
Intestinal ischemia/reperfusion (I/R)-injury often results in sepsis and organ failure is of major importance the clinic. A potential strategy to reduce I/R-injury application ischemic preconditioning (IPC) during which repeated, brief episodes I/R are applied. The aim this study was evaluate physiological cellular effects intestinal compare influence in-vivo IPC (iIPC) with ex-vivo (eIPC), blood derived factors nerval regulations excluded.Using an established perfused rat intestine model,...
Female gender is reported as an independent risk factor for a poor outcome after coronary artery bypass grafting. We analysed the influence of on surgical in patients with single-vessel disease undergoing minimally invasive direct (MIDCAB).From January 1998 to December 2016, total 607 consecutive (31.9% women) underwent MIDCAB at our institution. Major adverse cardiac and cerebrovascular events (MACCE) were recorded during median follow-up period 8.0 years. Survival time was estimated all...
Abstract Background Numerous tissue-derived factors have been postulated to be involved in tissue migration of circulating monocytes. The aim this study was evaluate whether a defined hypoxic gradient can induce directed naïve human monocytes and identify responsible autocrine/paracrine factors. Methods Monocytes were isolated from peripheral blood mononuclear cells, transferred into chemotaxis chambers subjected oxygen with or without the addition CCL26. Cell recorded secretome analyses...
Abstract Background Blood transfusions are common medical procedures and every age group requires detailed insights treatment bundles. The aim of this study was to examine the association anaemia, co-morbidities, complications, in-hospital mortality, transfusion according groups identify patient who particularly at risk when undergoing surgery. Methods Data from 21 Hospitals Patient Management Network Registry were analysed. Patients divided into subgroups. incidence preoperative surgical...