A5101732491- Acute Myeloid Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Protein Degradation and Inhibitors
- Diet, Metabolism, and Disease
- Metabolism, Diabetes, and Cancer
- Plant Pathogenic Bacteria Studies
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Transgenic Plants and Applications
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Immune cells in cancer
- Histone Deacetylase Inhibitors Research
- Plant-Microbe Interactions and Immunity
- Pituitary Gland Disorders and Treatments
- Chromosomal and Genetic Variations
- Nematode management and characterization studies
- RNA regulation and disease
- PI3K/AKT/mTOR signaling in cancer
- Nuclear Receptors and Signaling
- Neuroblastoma Research and Treatments
Memorial Sloan Kettering Cancer Center
2021-2025
Kettering University
2020-2024
Fujian Agriculture and Forestry University
2023
Ministry of Education of the People's Republic of China
2023
Jiangxi Agricultural University
2023
Gannan Normal University
2019-2022
Cornell University
2020-2021
Zhejiang University
2018-2021
Weill Cornell Medicine
2020-2021
John Jay College of Criminal Justice
2010
Genetic variants of the SLC6A3 gene that encodes human dopamine transporter (DAT) have been linked to a variety neuropsychiatric disorders, particularly attention deficit hyperactivity disorder. In addition, homozygous Slc6a3 knockout mouse displays phenotype. Here, we analyzed 2 unrelated consanguineous families with infantile parkinsonism-dystonia (IPD) syndrome and identified missense mutations (p.L368Q p.P395L) in both families. Functional studies demonstrated were loss-of-function...
Dysregulated gene expression contributes to most prevalent features in human cancers. Here, we show that subtypes of acute myeloid leukemia (AML) depend on the aberrant assembly MYB transcriptional co-activator complex. By rapid and selective peptidomimetic interference with binding CBP/P300 MYB, but not CREB or MLL1, find leukemic functions are mediated by co-activation a distinct set transcription factor complexes. These complexes assemble aberrantly LYL1, E2A, C/EBP family members, LMO2,...
In recent years, a large amount of clinical and experimental data has shown that M2-like polarized tumor-associated macrophages (TAMs) play an important role in cancer metastasis. Therefore, TAMs, especially TAMs is promising target for anti-tumor metastasis therapy. Here, we found celastrol dose-dependently suppressed IL-13 induced CD206 expression both RAW264.7 primary macrophages. Consistently, also inhibited the specific genes, including MRC1, Arg1, Fizz1, Mgl2 CD11c. Further, by...
Cancer stem cells are essential for initiation and therapy resistance of many cancers, including acute myeloid leukemias (AML). Here, we apply functional genomic profiling to diverse human leukemias, high-risk MLL- NUP98-rearranged specimens, using label tracing in vivo. Human leukemia propagation is mediated by a rare quiescent label-retaining cell (LRC) population undetectable current immunophenotypic markers. AML quiescence reversible, preserving genetic clonal competition epigenetic...
Abstract Background Fructose is an abundant source of carbon and energy for cells to use metabolism, but only certain cell types fructose proliferate. Tumor that acquire the ability metabolize have a fitness advantage over their neighboring cells, proteins mediate metabolism in this context are unknown. Here, we investigated determinants fructose-mediated proliferation. Methods Live imaging crystal violet assays were used characterize several lines (RKO, H508, HepG2, Huh7, HEK293T (293T),...
Phosphorylation (P-) of cAMP-response element-binding protein (CREB) by kinase A or mitogen-activated kinases was implicated in mediating the increased tyrosine hydroxylase (TH) gene expression after prolonged exposure to nicotine vivo and cell culture. We examined time course signaling pathways for phosphorylation CREB possible involvement ATF-2. Treatment PC12 cells with 200 microm triggered rapid but transient elevation P-CREB followed a second sustained rise 2-5 h continuous nicotine. In...
Our previous work suggested a role for oligomerization in regulating dopamine transporter (DAT) internalization, with d-amphetamine dissociating DAT oligomers and monomers being endocytosed. This model was put to detailed testing the present use of constructs differentially tagged Myc or Flag, reversal tags co-immunoprecipitation cross-linking assays, application antibodies against different biotinylation experiments. Upon pairing wild-type (WT) W84L mutant, effects on (decrease) but not...
Xanthomonas citri subsp. (Xcc) is the causal agent of citrus bacterial canker (CBC), one most devastating diseases. Most commercial varieties are susceptible to CBC. However, some and wild germplasms CBC resistant promising in genetic increases resistance against We aimed evaluate for First, we developed a rapid evaluation method based on enhanced yellow fluorescent protein (eYFP)-labeled Xcc. The results demonstrated that eYFP does not affect growth virulence Xcc-eYFP allows measurement...
Gastric cancer (GC), known for high morbidity and mortality, is poorly prognosed with traditional chemotherapy biological agents. Current studies have found that over-activation of AKT a common molecular characteristic in GC. Although the development this targeted inhibitor has entered clinical phases, limited success reported because its compensatory signaling pathways. Here, we GC cell lines phosphorylation show different sensitivity to inhibitors (AKTis), but reduction p-GSK3β related...
Abstract Chilli pepper is one of the most important and widely cultivated types in China. It has high nutritional economic value. Capsicum frutescens L. chilli production. In October 2022, a new disease showing blight entire plant was observed Nanchang City, Jiangxi Province. The fungal isolates consistently obtained from diseased samples were identified as Diaporthe caryae C. M. Tian & Qin Yang based on morphological characteristics, pathogenicity test phylogenetic analysis four genes,...
<title>Abstract</title> In eukaryotic cells, the retromer complex plays a crucial role in orchestrating sorting and retrograde transport of cargo proteins from endosomes to trans-Golgi network (TGN). Despite its significance, molecular details this intracellular trafficking process remain unclear. Here, we identified Golgi-associated protein (GARP) as mediator vesicular transport, facilitating recruitment TGN exert functions. The GARP is mainly localized TGN, where it interacts with complex....
Transposable elements (TEs) are abundant in the human genome, and they provide sources for genetic functional diversity. The regulation of TEs expression their consequences physiological conditions cancer development remain to be fully elucidated. Previous studies suggested repressed by DNA methylation chromatin modifications. effect 3D topology on TE remains elusive. Here, integrating transcriptome genome architecture studies, we showed that haploinsufficient loss
MYB fusions are recurrently found in select cancers, including blastic plasmacytoid dendritic cell neoplasm (BPDCN), an acute leukemia with poor prognosis. They markedly enriched BPDCN compared to other blood and some patients the only obvious somatic mutation detected. This suggests they may alone be sufficient drive transformation. hypothesized alter normal transcription factor activity of MYB, but mechanistically how promote leukemogenesis is poorly understood. Using CUT&RUN chromatin...
Larvae of click beetles are the most important soil-dwelling agricultural pests and abundant throughout world. Agrypnus sp. belongs to subfamily Agrypninae Elateridae. Here, we sequenced annotated complete mitochondrial genome (mitogenome) sp., new representative mitogenome subfamily. This was 16,056 bp long encoded 13 protein-coding genes (PCGs), 22 transfer RNA (tRNAs), two ribosomal unit (rRNAs). Gene order conserved identical other previously The nucleotide composition biases towards A...
Abstract Many human cancers, including acute myeloid leukemia (AML), arise from mutations of stem and progenitor cells. Immunophenotypic profiling has shown that leukemias develop hierarchically, with in cells associated disease propagation relapse 1,2 . Although initiating can be enriched using cell surface markers, their frequency tends to variable low, obscuring mechanisms hindering effective therapies 3,4 To define AML patients, we performed functional genomic diverse label tracing...
Xanthomonas citri subsp. ( Xcc ) is the agent of citrus bacterial canker (CBC) disease, which has significantly reduced quantity and quality in many producing areas worldwide. Copper-based bactericides are primary products for CBC control management, but problems derived from copper-resistant environmental contamination have become issues anxiety. Thus, there a need to find alternative antibacterial instead relying on single type agent. This study developed method evaluate inhibition agents...
Our previous studies suggest that ACTH play a crucial role in the regulation of catecholamine (CA) biosynthetic enzymes SCG, since its administration was as effective immobilization stress (IMO) increasing expression tyrosine hydroxylase (TH) and dopamine ß-hydroxylase (DBH) rat SCG. Moreover IMO increases receptors (MC2) SCG which directly bind ACTH. We performed several experiments to examine if has direct effect on CA enzyme gene expression. Adrenalectomy (ADX) led robust elevation plasma...
ABSTRACT Dysregulated gene expression contributes to most prevalent features in human cancers. Here, we show that subtypes of acute myeloid leukemia (AML) depend on the aberrant assembly MYB transcriptional co-activator complex. By rapid and selective peptidomimetic interference with binding CBP/P300 MYB, but not CREB or MLL1, find leukemic functions are mediated by co-activation a distinct set transcription factor complexes. These complexes assemble aberrantly LYL1, E2A, C/EBP family...
<title>Abstract</title> <bold>Background: </bold>Fructose is an abundant source of carbon and energy for cells to use metabolism, but only certain cell types fructose proliferate. Tumor that can metabolize have a fitness advantage over their neighboring cells, the proteins mediate metabolism in this context are unknown. Here, we investigated determinants fructose-mediated proliferation.<bold>Methods:</bold> We quantified ability 14 lines proliferate fructose-containing media (i.e....