A5014135504- Polymer crystallization and properties
- biodegradable polymer synthesis and properties
- Cancer Genomics and Diagnostics
- Polymer Nanocomposites and Properties
- Animal Genetics and Reproduction
- Metabolism, Diabetes, and Cancer
- Cancer-related Molecular Pathways
- Polymer composites and self-healing
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Cancer-related gene regulation
- Diet, Metabolism, and Disease
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Bioinformatics and Genomic Networks
- Nutrition and Health in Aging
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Wnt/β-catenin signaling in development and cancer
- Epigenetics and DNA Methylation
- interferon and immune responses
- Peptidase Inhibition and Analysis
- Synthesis and properties of polymers
- Advanced Breast Cancer Therapies
- Virus-based gene therapy research
Avera Health
2024
Cornell University
2016-2021
Weill Cornell Medicine
2017-2021
Memorial Sloan Kettering Cancer Center
2018-2021
Molecular Oncology (United States)
2021
Tri-Institutional PhD Program in Chemical Biology
2018-2020
Marshall University
2018
Kettering University
2018
NewYork–Presbyterian Hospital
2018
Presbyterian Hospital
2018
Excessive consumption of beverages sweetened with high-fructose corn syrup (HFCS) is associated obesity and an increased risk colorectal cancer. Whether HFCS contributes directly to tumorigenesis unclear. We investigated the effects daily oral administration in adenomatous polyposis coli (APC) mutant mice, which are predisposed develop intestinal tumors. The HFCS-treated mice showed a substantial increase tumor size grade absence metabolic syndrome. concentrations fructose glucose lumen...
Abstract Defining the genetic drivers of cancer progression is a key in understanding disease biology and developing effective targeted therapies. Chromosome rearrangements are common feature human malignancies, but whether they represent bona fide therapeutically actionable targets, requires functional testing. Here, we describe generation transgenic, inducible CRISPR-based mouse systems to engineer study recurrent colon cancer-associated EIF3E–RSPO2 PTPRK–RSPO3 chromosome vivo . We show...
The cancer anorexia cachexia syndrome is a systemic metabolic disorder characterized by the catabolism of stored nutrients in skeletal muscle and adipose tissue that particularly prevalent nonsmall cell lung (NSCLC). Loss results functional impairments increased mortality. aim present study was to characterize changes metabolism genetically engineered mouse model NSCLC. We show portion these animals develop loss muscle, tissue, inflammatory markers mirroring human syndrome. Using noncachexic...
Despite recent clinical successes for irreversible drugs, potential toxicities mediated by unpredictable modification of off-target cysteines represents a major hurdle expansion covalent drug programs. Understanding the proteome-wide binding profile inhibitors can significantly accelerate their development; however, current mass spectrometry strategies typically do not provide direct, amino acid level readout activity complex, selective inhibitors. Here we report development CITe-Id, novel...
Triple-negative breast cancers (TNBC) are genetically characterized by aberrations in
The WNT pathway is a fundamental regulator of intestinal homeostasis, and hyperactivation signaling the major oncogenic driver in colorectal cancer. To date, there are no described mechanisms that bypass dependence tumors. Here, we show although suppression blocks tumor growth most organoid vivo cancer models, accumulation cancer-associated genetic alterations enables drug resistance WNT-independent growth. In epithelial cells harboring mutations KRAS or BRAF, together with disruption TP53...
Inactivation of the tumor suppressor lipid phosphatase INPP4B is common in triple-negative breast cancer (TNBC). We generated a genetically engineered TNBC mouse model deficient INPP4B. found dose-dependent increase incidence homozygous and heterozygous knockout mice compared with wild-type (WT), supporting role for as TNBC. Tumors derived from are enriched AKT MEK gene signatures. Consequently, deficiency more sensitive to PI3K or inhibitors WT mice. Mechanistically, we that increases...
Abstract The areas under the linear loss modulus versus temperature curves (loss area, LA) and tan δ (TA) were evaluated for a number of acrylic, methacrylic, styrenic butadiene based copolymers interpenetrating polymer networks, IPNs, as function crosslink density comliosition, compared with values predicted by group contribution analysis. LAs sequential cross ‐poly(n‐butyl methacrylate)‐ inter ‐ polystyrene, found to exhibit up 30% larger than poly(n‐butyl metacrylate‐ stat ‐styrene)...
The use of naturally functionalized triglyceride oils in interpenetrating polymer networks (IPNs) is reviewed. An oil bearing either hydroxyl or epoxide functionality may be crosslinked to form a soft elastomer the presence another monomer network an IPN, linear polymer, semi‐IPN. Polymerization and characterization are mentioned, with emphasis on distribution effect nontrifunctional triglycerides properties. simultaneous synthesis polystyrene/functional IPNs reviewed, several factors...
Abstract Background Fructose is an abundant source of carbon and energy for cells to use metabolism, but only certain cell types fructose proliferate. Tumor that acquire the ability metabolize have a fitness advantage over their neighboring cells, proteins mediate metabolism in this context are unknown. Here, we investigated determinants fructose-mediated proliferation. Methods Live imaging crystal violet assays were used characterize several lines (RKO, H508, HepG2, Huh7, HEK293T (293T),...
The tissue-specific expression of major histocompatibility complex class I genes is determined by a series upstream regulatory elements, many which remain ill defined. We now report that distal E-box element, located between bp −309 and −314 transcription initiation, acts as cell type-specific enhancer promoter activity. E box very active in neuroblastoma line, CHP-126, but relatively inactive the HeLa epithelial line. basic helix-loop-helix leucine zipper proteins stimulatory factor 1...
Human neuronal cells express neither major histocompatibility complex (MHC) class I RNA nor cell surface molecules but can be induced to do so by various cytokines. In the present studies, we report that expression of MHC in a neuroblastoma line, CHP-126, is actively repressed. This repression mediated combined effects series upstream silencer elements. Removal silencers reveals not only an active promoter element also presence enhancer. Four have been identified and shown distinct...
Abstract The homopolymers, poly(3,3‐bisethoxymethyl oxetane) (polyBEMO), poly(3,3‐bisazidomethyl (polyBAMO), and triblock copolymers based on these homopolymers a statistical copolymer center block composed of BAMO 3‐azidomethyl‐3‐methyl oxetane AMMO were synthesized characterized by differential scanning calorimetry, modulus‐temperature, optical microscopy, membrane osmometry, solution melt viscosity. values K for the Mark‐Houwink equation found to be 7.29 × 10 −3 mL/g 0.80, respectively,...
Abstract Two crystal modifications have been found for poly(3,3‐bisethoxymethyl oxetane) [poly‐ (BEMO)] by wide‐angle x‐ray powder diffraction and differential scanning calorimetry, while only one modification has poly(3,3‐bisazidomethyl [poly(BAMO)]. Melting temperatures the two polymers were nearly same, varying from about 70 to 90°C depending on thermal treatment; higher crystallization resulted in melting temperatures. The equilibrium temperature T was be 125 128°C poly(BEMO) poly(BAMO),...
Abstract The thermal decomposition behavior of poly[3,3‐bis(ethoxymethyl)oxetane] (polyBEMO) was examined and compared to the poly(ethylene oxide) (PEO) poly(tetramethylene (polyTHF). Differential scanning calorimetric (DSC) studies as a function heating rates at constant temperature time yielded activation energies 45–50 kcal/mol, characteristic polyether decomposition. First‐order kinetics were found. reaction is endothermic, with heat 18.6 kcal/mol. Effusion mass spectroscopy on polyBEMO...
Abstract Purpose Fusion genes can be therapeutically relevant if they result in constitutive activation of oncogenes or repression tumor suppressors. However, the prevalence and role fusion female cancers remain largely unexplored. Here, we investigate gene landscape triple-negative breast cancer (TNBC) high-grade serous ovarian (HGSOC), two subtypes with high molecular similarity but limited treatment options at present. Methods RNA-seq was utilized to identify a cohort 18 TNBC HGSOC...
Abstract Semi‐interpenetrating polymer networks based on two elastomers, cis ‐1,4‐polyisoprene (PI) and thermoplastic polyurethane elastomers (TPUs) were prepared in varying compositions. The PI component was cross‐linked using peroxide initiators. Modulus mechanical properties investigated as a function of composition temperature. Slight synergisms observed properties, particularly for compositions containing 10% by weight. Little or no molecular mixing is shown differential scanning...
Diblock, triblock, and alternating block copolymers based on poly[3,3-bis(ethoxymethyl) oxetane] [poly(BEMO)] a random copolymer center poly(BMMO-co-THF) composed of poly[3,3-bis(methoxymethyl)oxetane] [poly(BMMO)], poly(tetrahydrofuran) [poly(THF)] were synthesized characterized with respect to molecular weight. Glass transition temperatures Tg melting Tm via DSC, modulus–temperature, dynamic mechanical spectroscopy (DMS). These polyethers had between 70°C 90°C, −55°C −30°C. The degree...
ABSTRACT The WNT pathway is a fundamental regulator of intestinal homeostasis and hyperactivation signaling the major oncogenic driver in colorectal cancer (CRC). To date, there are no described mechanisms that bypass dependence tumors. Here, we show while suppression blocks tumor growth most organoid vivo CRC models, accumulation CRC-associated genetic alterations enables drug resistance WNT-independent growth. In epithelial cells harboring mutations KRAS or BRAF, together with disruption...
Abstract The relationships between equilibrium melting temperatures ( T m *) and crystalline morphology of poly[3,3bis(ethoxymethyl)oxetane], (polyBEMO), poly[3,3‐bis (azidomethyl)oxetane], (polyBAMO), poly(ethylene oxide), (PEO), were characterized. Hoffman‐Weeks extrapolation procedure led to * values 92, 102, 69°C for these three polymers, respectively. gross morphologies polyBEMO, polyBAMO, PEO studied as a function melt‐liquid temperature i ) isothermal crystallization c by visible...
Abstract Interpenetrating polymer networks are combinations of two crosslinked polymers. Often, they exhibit dual phase continuity, meaning that both phases extend continuously throughout the sample. Recent research on IPNs has shown nucleation and spinodal decomposition mechanisms important in kinetics separation. If polymers almost miscible, a microheterogeneous morphology will develop. Under these conditions, glass transition IPN may be very broad, extending range between homopolymers....