A5071453706- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Nanocluster Synthesis and Applications
- DNA and Nucleic Acid Chemistry
- Nanoparticle-Based Drug Delivery
- Phytochemicals and Antioxidant Activities
- Metabolism, Diabetes, and Cancer
- Gold and Silver Nanoparticles Synthesis and Applications
- Pancreatic function and diabetes
- Supramolecular Self-Assembly in Materials
- MicroRNA in disease regulation
- Acute Kidney Injury Research
- Cancer, Hypoxia, and Metabolism
- Electrochemical Analysis and Applications
- Nanopore and Nanochannel Transport Studies
- Diabetic Foot Ulcer Assessment and Management
- Aluminum toxicity and tolerance in plants and animals
- Heme Oxygenase-1 and Carbon Monoxide
- Telomeres, Telomerase, and Senescence
- Apelin-related biomedical research
- Evaluation and Optimization Models
Soochow University
2021-2025
Hubei University
2024
Shanxi Medical University
2017-2023
Shanxi Academy of Medical Sciences
2022-2023
National University of Singapore
2020-2022
Early Warning (United States)
2022
Taiyuan Central Hospital
2022
Beijing Information Science & Technology University
2021
Xiamen University
2019
Huazhong University of Science and Technology
2013-2017
Through rational design of a functional molecular probe with high sequence specificity that takes advantage sensitive isothermal amplification simple operation, we developed one-pot hairpin-mediated quadratic enzymatic strategy for microRNA (miRNA) detection. Our method exhibits ultrahigh sensitivity toward miR-21 detection limits 10 fM at 37 °C and 1 aM 4 °C, which corresponds to nine strands in 15 μL sample, it is capable distinguishing among miRNA family members. More importantly, the...
Pancreatic β cell dysfunction and reduction due to glucose toxicity play a crucial role in the development of type 2 diabetes mellitus (T2DM). Irisin, novel exercise-induced myokine, reduces obesity, improves insulin resistance lowers blood by promoting browning white adipose tissue, thereby enhancing thermogenesis increasing energy expenditure. Recent studies have reported that irisin promotes proliferation protects cells from apoptosis. However, effects on pancreatic are unknown. Thus, aim...
Acute kidney injury (AKI) induced by ischemia reperfusion is closely related to mitochondrial dysfunction. Nicotinamide adenine dinucleotide (NAD+ ) can enhance the function and restrain following inflammation, but it hardly delivered lacks renal targeting ability. To address these problems, herein, an ultrasmall Fe3 O4 nanoparticle used as a carrier deliver nicotinamide mononucleotide (NMN), precursor of NAD+ . An outstanding sophistication current design that once NMN attached on surface...
Radiotherapy (RT) hinges on DNA damage-induced cancer cell death and the subsequent anti-tumor immunity. However, efficacy of RT is curtailed by cycle heterogeneity an immunosuppressive tumor microenvironment, which foster radioresistance. Here ion generator-based enhancement strategy demonstrated in a mouse model radioresistant tumor. The generator degraded resulting iron-triggered ferroptosis that enhanced immunogenic manganese-activated stimulator interferon gene reversed environment. As...
The recently developed DNA−gold nanoparticle (DNA−GNP) biobarcode assay provides polymerase chain reaction (PCR)-like sensitivity for nucleic acid and protein targets without a need enzymatic amplification. However, application of the conventional is challenged by its complex, expensive, time-consuming, labor-intense procedure. Herein, we present new electrochemiluminescence (ECL) method based on cysteamine−GNP conjugates. In this strategy, an ECL nanoprobe fabricated that relies GNP...
3D cross-linked DNA superstructures switch off the ionic flux through solid-state nanopores with extremely high ON–OFF ratios of 103–105. This gating mechanism can be generally applicable in a wide range opening diameters up to 650 nm. The bio-supramolecular gatekeepers outperform previous low-dimensional or simple-structured functional components.
Telomerase, a valuable biomarker, is highly correlated with the development of most human cancers. Here, we develop bidirectional strategy for telomerase activity detection and bladder cancer diagnosis based on four detection-color states difunctional gold nanoparticle (GNP) probes such as blue, purple, red, precipitate. Specifically, define red GNP probe origin, which represents urine extracts inactive implies normal individuals. The forward direction corresponding to relatively high...
Telomerase is a widely used tumor biomarker for early cancer diagnosis. On the basis of combined use aggregation-induced emission (AIE) fluorogens and quencher, quencher group induced high specificity strategy detection telomerase activity from cell extracts patients' urine specimens was creatively developed. In absence telomerase, fluorescence background extremely low due to short distance between AIE dye. addition enhances significantly. The in E-J, MCF-7, HeLa equivalent 5-10 000 cells...
Herein, we report natural chitosan end-capped MCM-41 type MSNPs as novel, dual stimuli, responsive nano-vehicles for controlled anticancer drug delivery. The nanovalves tightly close the pores of to control premature cargo release under physiological conditions but respond lysozyme and acidic media trapped cargo.
Abstract Diabetic ulcers (DUs) appearing as chronic wounds are difficult to heal due the oxidative stress in wound microenvironment and their high susceptibility bacterial infection. A routine treatment combining surgical debridement with anti‐infection therapy is widely used for treating DUs clinic, but hardly offers a satisfying healing outcome. It known that long‐term antibiotic may also lead drug resistance of pathogens. To address these challenges, new strategies both reactive oxygen...
Herein, we design novel cellulose conjugated mesoporous silica nanoparticle (CLS-MSP) based nanotherapeutics for stimuli responsive intracellular doxorubicin (DOX) delivery. DOX molecules are entrapped in pores of the fabricated nanoparticles (MSPs) while is used as an encapsulating material through esterification on outlet MSPs to avoid premature release under physiological conditions. In vitro studies, successfully achieved from loaded (DOX/CLS-MSPs) by pH and cellulase triggers....
Vaspin (visceral adipose tissue-derived serine protease inhibitor) is a recently discovered adipokine that has been implicated in diabetes mellitus and other metabolic disorders. However, the effects of vaspin on pancreatic β cell function related mechanisms are not fully understood. Thus, present study was performed to investigate potential underlying mechanisms. Both vitro (rat insulinoma cells, INS-1) vivo (high fat diet fed rats) experiments were conducted. The results showed...
Abstract Insulin resistance (IR) is the main pathogenesis of metabolic syndrome and a shared pathophysiological change in conditions such as diabetes mellitus, adiposity, hypertension, atherosclerosis. Visceral adipose tissue-derived serpin (Vaspin) newly discovered adipocytokine with insulin-sensitizing anti-inflammatory effects. To examine if vaspin can improve insulin rats fed high-fat diet via receptor substrate/phosphatidylinositol 3 kinase/protein kinase B/glucose transport...
We combine the telomerase extension reaction and microRNA (miRNA)-induced rolling circle amplification, followed by graphene oxide (GO) nicking enzyme-assisted signal amplification as a method to analyze miRNA-21 in urine samples with following merits. First, it is binary assay can simultaneously output double signals that correspond quantities of miRNA, respectively. Second, activity enhanced using DNA molecular beacon probe inhibit formation G-quadruplex. Third, background noise decreased...
Efforts on bladder cancer treatment have been shifting from extensive surgery to organ preservation in the past decade. To this end, we herein develop a multifunctional nanoagent for downstaging and bladder-preserving therapy by integrating mucosa penetration, reduced off-target effects, internal irradiation into nanodrug. Specifically, an iron oxide nanoparticle was used as carrier that coated with hyaluronic acid (HA) facilitating penetration. Dibenzocyclooctyne (DBCO) introduced HA...
Chirality is one of the fundamental biochemical properties in a living system, and lot biological physiological processes are greatly influenced by chirality molecules. Inspired this phenomenon, we study covalent assembly DNA on chiral molecule modified surfaces further discuss hybridization with nucleic acids. Take methylene blue (MB) as model molecule, show that peak current L-NIBC (NIBC, N-isobutyryl-L(D)-cysteine) gold surface (L-surface) larger than D-surface because stronger...
Recently, the incorporation of biomolecules in Metal-organic frameworks (MOFs) attracts many attentions because controlling functions, properties and stability trapped molecules. Although there are few reports on protein/MOFs composites their applications, none DNA/MOFs composite is reported, as far we know. Here, report a new material which self-assembled from 3D DNA (guest) pre-synthesized MOFs (host) by electrostatic interactions hydrophilic well-dispersed fashion. Its biophysical...
We report a simple colorimetric method using gold nanoparticles as signal generators for the real-time detection of product strand displacement cascade.
Biocomputing, a subarea of unconventional chemical computing, is performed by DNA, protein/enzymes, and other living organisms. Recently, various biomolecular logic gates employing optical changes or PAGE measurements have been studied intensively using inputs. However, the low detection speed, which an inherent characteristic method, has prevented it from being developed technologically. Most reported to date are mainly based on fluorescence, phosphorescence, colorimetric outputs,...
Reprogrammed glucose metabolism is vital for cancer cells, but aspartate, an intermediate metabolic product, the limiting factor cell proliferation. However, due to complexity of pathways, it remains unclear whether primary source endogenous aspartate. Here, we report design innovative molecular deactivator, based on a multifunctional upconversion nanoprobe, explore link between and This deactivator mainly works in acidic, hypoxic tumor microenvironment deactivates multiple types...