A5048396749- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Coenzyme Q10 studies and effects
- Free Radicals and Antioxidants
- Computational Drug Discovery Methods
- ATP Synthase and ATPases Research
- Cholinesterase and Neurodegenerative Diseases
- Liver Disease Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Sirtuins and Resveratrol in Medicine
- Alzheimer's disease research and treatments
- Phytochemicals and Antioxidant Activities
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Telemedicine and Telehealth Implementation
- Cancer, Hypoxia, and Metabolism
- Biochemical Acid Research Studies
- Lipoproteins and Cardiovascular Health
- Genomics, phytochemicals, and oxidative stress
- Electrochemical sensors and biosensors
- Metabolomics and Mass Spectrometry Studies
- Vitamin C and Antioxidants Research
- Sulfur Compounds in Biology
- Birth, Development, and Health
- Diet and metabolism studies
University of Coimbra
2017-2025
University of Trás-os-Montes and Alto Douro
2011-2025
ATP Clinical Research (United States)
2024
Universidade do Porto
2012-2022
Biocant
2017-2022
Radboud University Nijmegen
2015-2020
Radboud University Medical Center
2015-2020
Radboud Institute for Molecular Life Sciences
2015-2020
Hospital TotalCor
2015
MRC Mitochondrial Biology Unit
2012
The olive oil industry generates large quantities of cake (OC), making its use in animal feed an environmentally sustainable alternative. Considering that the ham Bísaro pigs is traditionally used for production cured ham, analysis raw meat essential to understand how diet influences quality before maturation process. This study examined effect different types OC diets pigs, focusing on chemical compositions and fatty acid profiles three fresh muscles-biceps femoris (BF), semimembranosus...
The extracellular pH (pHe) is a key determinant of the cellular (micro)environment and needs to be maintained within strict boundaries allow normal cell function. Here we used HEK293 cells study effects pHe acidification (24 h), induced by mitochondrial inhibitors (rotenone, antimycin A) and/or HCl addition. Lowering from 7.2 5.8 reduced viability 70% was paralleled decrease in cytosolic (pHc), hyperpolarization membrane potential (Δψ), increased levels hydroethidine-oxidizing ROS...
Inhibitor studies with isolated mitochondria demonstrated that complex I (CI) and III (CIII) of the electron transport chain (ETC) can act as relevant sources mitochondrial reactive oxygen species (ROS). Here we studied ROS generation oxidative stress induction during chronic (24h) inhibition CI CIII using rotenone (ROT) antimycin A (AA), respectively, in intact HEK293 cells. Both inhibitors stimulated oxidation sensor hydroethidine (HEt) increased NAD(P)H levels without major effects on...
Targeting mitochondrial oxidative stress is an effective therapeutic strategy. In this context, a rational design of mitochondriotropic antioxidants (compounds 22-27) based on dietary antioxidant (caffeic acid) was performed. Jointly named as AntiOxCINs, these molecules take advantage the known ability triphenylphosphonium cation to target active mitochondria. The study guided by structure-activity-toxicity-property relationships, and we demonstrate in work that novel AntiOxCINs act...
The progression of non-alcoholic fatty liver (NAFL) into steatohepatitis implicates multiple mechanisms, chief which is mitochondrial dysfunction. However, the sequence events underlying failure are still poorly clarified. In this work, male C57BL/6J mice were fed with a high-fat plus high-sucrose diet for 16, 20, 22, and 24 weeks to induce NAFL. Up 20th week, an early remodeling increased OXPHOS subunits levels higher respiration occurred. Interestingly, progressive loss along “Western...
Non-alcoholic fatty liver disease (NAFLD) is a health concern affecting 24% of the population worldwide. Although pathophysiologic mechanisms underlying are not fully clarified, mitochondrial dysfunction and oxidative stress key players in progression. Consequently, efforts to develop more efficient pharmacologic strategies targeting mitochondria for NAFLD prevention/treatment underway. The conjugation caffeic acid anti-oxidant moiety with an alkyl linker triphenylphosphonium cation (TPP+),...
Background and Aims: Receptor‐interacting protein kinase 3 (RIPK3) mediates NAFLD progression, but its metabolic function is unclear. Here, we aimed to investigate the role of RIPK3 in modulating mitochondria function, coupled with lipid droplet (LD) architecture NAFLD. Approach Results: Functional studies evaluating LD biology were performed wild‐type (WT) Ripk3 −/− mice fed a choline‐deficient, amino acid‐defined (CDAA) diet for 32 66 weeks CRISPR‐Cas9 ‐null fat‐loaded immortalized...
The discovery of new chemical entities endowed with potent and selective acetylcholinesterase (AChE) and/or butyrylcholinesterase (BChE) inhibitory activity is still a relevant subject for Alzheimer's disease therapy. Therefore, small library benzoic based amide nitrones (compounds 24 to 42) was synthesized screened toward cholinesterase enzymes. SAR studies showed that the tert-butyl moiety most favourable nitrone pattern. In general, derivatives effectively inhibited AChE, being compound...
Oxidative stress and mitochondrial dysfunction have been associated with metabolic age-related diseases. Thus, the prevention of oxidative damage is nowadays a recognized pharmacological strategy to delay disease progression. Epidemiological studies suggested an association between consumption polyphenol-rich diet different pathologies, including diseases etiology. The development mitochondrial-targeted antioxidants based on dietary may decrease damage. Herein, we report design synthesis two...
Alzheimer's disease (AD) is a multifactorial age-related associated with oxidative stress (OS) and impaired cholinergic transmission. Accordingly, targeting mitochondrial OS restoring transmission can be an effective therapeutic strategy toward AD. Herein, we report for the first time dual-target hydroxybenzoic acid (HBAc) derivatives acting as mitochondriotropic antioxidants cholinesterase (ChE) inhibitors. The studies were performed two AntiOxBEN1 (catechol derivative), AntiOxBEN2...
A novel mitochondria-targeted antioxidant (TPP-OH) was synthesized by attaching the natural hydrophilic caffeic acid to an aliphatic lipophilic carbon chain containing a triphenylphosphonium (TPP) cation. This compound has similar activity as demonstrated measurement of DPPH/ABTS radical quenching and redox potentials, but is significantly more hydrophobic than its precursor indicated relative partition coefficients. The both compounds intrinsic related ortho-catechol system, methoxylation...
Although the lipophilic triphenylphosphonium (TPP+) cation is widely used to target antioxidants mitochondria, TPP+-based derivatives have shown cytotoxicity in several biological vitro models. We confirmed that Mito.TPP cytotoxic both human neuronal (SH-SY5Y) and hepatic (HepG2) cells, decreasing intracellular adenosine triphosphate (ATP) levels, leading mitochondrial membrane depolarization reduced mass after 24 h. surpassed this concern using nitrogen-derived cationic carriers (Mito.PICO,...