A5054876999- Heart Failure Treatment and Management
- Nitric Oxide and Endothelin Effects
- Bone Metabolism and Diseases
- Connexins and lens biology
- Receptor Mechanisms and Signaling
- Protein Hydrolysis and Bioactive Peptides
- Renin-Angiotensin System Studies
- Heat shock proteins research
- Bone health and treatments
- Protease and Inhibitor Mechanisms
- Hormonal Regulation and Hypertension
- Peptidase Inhibition and Analysis
- Ion channel regulation and function
- Retinoids in leukemia and cellular processes
- Diabetes Treatment and Management
- Molecular Biology Techniques and Applications
- Inflammatory mediators and NSAID effects
- Erythropoietin and Anemia Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Eicosanoids and Hypertension Pharmacology
- Estrogen and related hormone effects
- Adipokines, Inflammation, and Metabolic Diseases
- Pulmonary Hypertension Research and Treatments
- Iron Metabolism and Disorders
- Potassium and Related Disorders
Toin University of Yokohama
2009-2025
Nagoya City University
2015-2023
Fukuda Denshi (Japan)
2021-2022
Weatherford College
2021-2022
Nagoya City University Hospital
2020
Clinical Research Management
2020
Tokyo Institute of Technology
1999-2016
Institute for Rheumatic Diseases (Japan)
2010
Kawamura Hospital
2009
National Institute of Health Sciences
2007-2008
Effects of pre-germinated brown rice (PGBR) on streptozotocin-induced diabetic rats were studied. The feeding a PGBR diet to ameliorated the elevation blood glucose and PAI-1 concentrations significantly, tended decrease plasma lipid peroxide in comparison with fed white diet. These results suggest that intake instead is effective for prevention vascular complications.
The stromal cell line ST2, derived from mouse bone marrow, differentiated into osteoblast-like cells in response to ascorbic acid. Ascorbic acid induced alkaline phosphatase (ALPase) activity, the expression of mRNAs for proteins that are markers osteoblastic differentiation, deposition calcium, and formation mineralized nodules by ST2 cells. We investigated mechanism whereby differentiation Inhibitors collagen triple helices completely blocked effects on cells, an indication matrix type I...
Polyphenol have been reported to physiological effects with respect alleviating diseases such as osteoporosis and osteopetrosis. We recently that the olive polyphenol hydroxytyrosol accelerates bone formation both in vivo vitro. The present study was designed evaluate vitro of apigenin (4',5,7-trihydroxyflavone), one major polyphenols olives parsley, on by using cultured osteoblasts osteoclasts ovariectomized (OVX) mice, respectively. Apigenin markedly inhibited cell proliferation indices...
The effects of natriuretic peptides on the proliferation and differentiation osteoblast-like cells from rat calvariae were examined. Natriuretic are physiological agonists that activate receptor guanylate cyclases, namely, peptide (NPR)-A NPR-B. Exposure to atrial (ANP) C-type (CNP) resulted in large increases rate intracellular production guanosine 3',5'-cyclic monophosphate (cGMP). Moreover, CNP-like immunoreactivity was detected conditioned medium cells, while ANP undetectable. In exposed...
Although quercetin has suppressed bone resorption in several animal studies, its target cells and the mechanism of action related to not been fully elucidated. We investigated effect on differentiation activation osteoclasts. used cocultures mouse spleen ST2 cells, cultures osteoclast progenitor {M-CSF-dependent (MD) from marrow murine monocytic RAW 264 (RAW) cells}. Quercetin dose-dependently inhibited osteoclast-like (OCL) cell formation at 2—5 μM concentration both coculture MD culture....
Natriuretic peptide receptor-B (NPR-B) was identified in rat chondrocytes, and its physiological functions were investigated.Rat tissues, including the xiphoid cartilage, brain, lung, liver, adrenal gland, kidney, screened for NPR-B activity, which we assayed by receptor guanylate cyclase activity specifically stimulated C-type natriuretic (CNP), a known selective activator of NPR-B.Cartilage showed distinctly higher activity.Furthermore, exposure cultured chondrocytes to CNP M) resulted...
We examined the effects of angiotensin II (Ang II) on differentiation rat calvarial osteoblastic cells and formation bone by these cells. Northern blotting analysis revealed that Ang inhibited expression mRNA for osteocalcin, which is a protein specifically expressed during maturation decreased activity alkaline phosphatase, marker differentiation, in cells, acting via type 1 (AT1) receptor. used von Kossa staining to examine mineralized nodules Both number total area were quantified shown...
Abstract The ability of mammalian central nervous system (CNS) neurons to survive and/or regenerate following injury is influenced by surrounding glial cells. To identify the factors that control cell function CNS injury, we have focused on endothelin B receptor (ET R), which show expressed majority astrocytes are immunoreactive for acid fibrillary protein (GFAP) in both normal and crushed rabbit optic nerve. Optic nerve crush induces a marked increase ET R GFAP immunoreactivity (IR) without...
The receptor for atrial natriuretic peptide (ANP) was purified to apparent homogeneity from bovine lung by a combination of detergent extraction, ammonium sulfate precipitation, and affinity chromatography on ANP-Affi-Gel 10. Mr the is about 140,000 as determined sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After reduction, protein migrated single band with an near 70,000. NH2-terminal sequence analysis material revealed only one sequence, indicating that ANP composed two...
Significance Mammalian B lymphocytes make antibodies of five different heavy chain isotypes, IgM, IgD, IgG, IgE, and IgA. The isotypes are produced at discrete stages in B-cell development from a single immunoglobulin ( Igh ) gene, either by irreversible rearrangement the gene to or IgA alternative splicing RNA transcribed coexpress IgM IgD. Developmentally regulated trans-acting factors have been hypothesized control IgD expression large RNAs, but these remained elusive for several decades....
3-Methylcholanthrene (3MC) is a ligand for arylhydrocarbon receptor (AhR), which binds dioxin. We examined the effects of 3MC on proliferation and differentiation osteoblasts using cultures rat calvarial osteoblast-like cells (ROB cells) mouse clonal preosteoblastic (MC3T3-E1 cells). Analysis by RT-PCR revealed that mRNAs AhR nuclear translocators were expressed in both ROB MC3T3-E1 cells. Cell synthesis DNA markedly inhibited exposure to 3MC. Furthermore, reduced activity alkaline...
Abstract Apigenin is a flavonoid with antioxidant and anticancer effects. It has been reported that apigenin inhibits proliferation, migration, invasion induces apoptosis in cultured lung cancer cells. However, there little information on the involvement of microRNAs (miRNAs) its miRNA microarray analysis polymerase-chain-reaction miRNAs revealed treatment human A549 cells up-regulated level miR-34a-5p. Furthermore, mRNA results three microRNA target prediction tools showed Snail Family...
Abstract Osteoclast-like cells, in cocultures with mouse spleen cells and clonal osteogenic stromal ST2 are formed from monocyte/macrophage lineage response to a combination of osteoclast differentiation factor (RANKL) OPG, decoy receptor for RANKL, produced by 1α,25-dihydroxyvitamin D3. Treatment the natural isoflavonoid genistein 6 h before coculture inhibited formation tartrate-resistant acid phosphatase-positive osteoclast-like cells. When we measured levels RANKL mRNA found that...
A comparative study of the natriuretic‐peptide receptor NPR‐B was performed by cloning and expressing, in COS‐1 cells, subtype from eel gill which exhibited a strong C‐type‐natriuretic‐peptide(CNP)‐induced guanylate cyclase activity. Like other mammalian receptors, consisted ligand‐binding extracellular domain, hydrophobic transmembrane kinase‐like domain domain. Sequence comparison among receptors revealed relatively low similarity (∼44%) compared to very high (∼ 84 %) cytoplasmic...
It has been assumed that prostaglandin (PG)I2 signaling contributes to the negative growth control of lung cancer cells; however, mechanism remains unresolved. PGI2 functions through a cell surface G protein-coupled receptor (prostaglandin I2-binding receptor, IP) and also exerts an effect by interacting with nuclear hormone peroxisome proliferator-activated delta (PPARdelta). We found PPARdelta was key molecule give cells (A549), using carbarprostacyclin, agonist for IP PPARdelta, L-165041,...
Retinol, a morphogen, has been shown to induce morphological changes in vascular endothelial cells, accompanied by an acute and specific accumulation of 80-kDa protein; purification characterization this retinol-induced protein (RIP) have revealed that it is transglutaminase. Endothelial cells from bovine carotid artery were cultured, treated with retinol, examined for morphology profiles. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis extracts prepared retinol-treated...