Although (−)-epigallocatechin gallate (EGCG) has been reported to induce apoptosis in a variety of tumor cells, detailed mechanisms remain be explored. In the present study, we investigated antitumor mechanism EGCG by using human T-cell acute lymphoblastic leukemia Jurkat cells. We focused on involvement reactive oxygen species, as found previously that caused apoptotic cell death osteoclastic cells due mainly promotion reduction Fe(III) Fe(II) trigger Fenton reaction, which affords hydroxyl...

The ATP-binding cassette (ABC) transporter ABCG2 has been implicated to play a significant role in the response of patients medication and/or risk diseases. To clarify possible physiological or pathological relevance polymorphisms, we have functionally validated single nucleotide polymorphisms (SNP) ABCG2. In present study, based on currently available data SNPs and acquired mutations, created total 18 variant forms (V12M, G51C, Q126stop, Q141K, T153M, Q166E, I206L, F208S, S248P, E334stop,...

A girl with hyperglycinemia of nonketotic type was presented. The liver biopsied from the patient studied for glycine metabolism. It found that yield 14CO2 glyeine-l-14C and rate 11C incorporation into serine as well glycine-2-14C were extremely low in patient's than control livers, while showed normal activities serine-hydroxyrnethylase serine-dehydratase. These findings indicate primary lesion is a defect glyeine cleavage reaction which gives rise to formation CO2,...

Although quercetin has suppressed bone resorption in several animal studies, its target cells and the mechanism of action related to not been fully elucidated. We investigated effect on differentiation activation osteoclasts. used cocultures mouse spleen ST2 cells, cultures osteoclast progenitor {M-CSF-dependent (MD) from marrow murine monocytic RAW 264 (RAW) cells}. Quercetin dose-dependently inhibited osteoclast-like (OCL) cell formation at 2—5 μM concentration both coculture MD culture....

Abstract In order to clarify the mechanisms underlying changes in primary afferent neurons trigeminal neuropathic pain, a chronic constriction nerve injury model of infraorbital (ION‐CCI) was developed rats. Mechanical allodynia observed at 3 days after ION‐CCI and lasted more than 14 days. Single‐unit activities were recorded from ION anesthetized C‐, Aβ‐ Aδ‐units identified on basis their conduction velocity. frequently encountered later period ION‐CCI. The highest Aδ‐spontaneous activity...

The human ATP-binding cassette (ABC) transporter ABCG2 (BCRP/MXR1/ABCP) plays a critical role in cellular protection against xenobiotics as well pharmacokinetics of drugs our body. In the present study, we aimed to analyze quantitative structure-activity relationship (QSAR) latently residing ABCG2-drug interactions. We first established standard methods for expression insect cells, quality control plasma membrane samples by using electron microscopy techniques, and high-speed screening...

The human ATP-binding cassette (ABC) transporter, ABCG2 (BCRP/MXR/ABCP), is a plasma membrane protein containing intramolecular and intermolecular disulfide bonds an N-linked glycan at Asn596. We have recently reported that the bond critical checkpoint for determining degradation fates of ABCG2. In present study, we aimed to analyze quantitatively impact on stability For this purpose, incorporated one single copy cDNA into designated site genomic DNA in Flp-In-293 cells stably express or its...

One single-nucleotide polymorphism (SNP), 538G>A (Gly180Arg), in the ABCC11 gene determines type of earwax. The G/G and G/A genotypes correspond to wet earwax, whereas A/A corresponds dry type. Wide ethnic differences exist frequencies those alleles, reflecting global migratory waves ancestors humankind. We herein provide evidence that this genetic has an effect on N-linked glycosylation ABCC11, intracellular sorting, proteasomal degradation variant protein. Immunohistochemical studies with...

Human ABCG2 belongs to the ATP-binding cassette (ABC) transporter family and plays an important role in various biological reactions, such as xenobiotic elimination homeostasis of protoporphyrin. We previously reported that exists plasma membrane a homodimer bound via disulfide bond at Cys-603. In present study, we examined importance intramolecular for stability protein. Substitution either Cys-592 or Cys-608 located extracellular loop glycine resulted significant decrease protein levels...

The authors discuss the safety and efficacy of an ultrasonic bone curette in various spinal surgeries report its advantages clinical application.Between April 2002 September 2003, 76 patients with diseases (29 cervical, five thoracic, 40 lumbosacral, two foramen magnum regions) were treated microsurgically by using a Sonopet longitudinal torsional tips lightweight handpieces. operations performed successfully device was easy to handle. There no instrument-related complications or -induced...

This study was aimed at clarifying the effect of intraoperative hypotensive anesthesia on outcome early surgery in patients with subarachnoid hemorrhage (SAH) caused by saccular cerebral aneurysms. Other factors were also screened for possible effects outcome.Hospital charts 84 consecutive SAH who underwent aneurysm clipping Day 4 examined. Possible affecting analyzed using multiple logistic regression dichotomous Glasgow Outcome Scale score as variable. The relationship between hypotension...

Mature bone-resorbing osteoclasts (OCs) mediate excessive bone loss seen in several disorders, including osteoporosis. Here, we showed that reveromycin A (RM-A), a small natural product with three carboxylic groups its structure, induced apoptosis specifically OCs, but not OC progenitors, nonfunctional osteoclasts, or osteoblasts. RM-A inhibited protein synthesis OCs by selectively blocking enzymatic activity of isoleucyl-tRNA synthetase. The proapoptotic effect was neutralization disruption...

Clinical relevance is implicated between the genetic polymorphisms of ABC (ATP-binding cassette) transporter ABCG2 (ABC subfamily G, member 2) and individual differences in drug response. We expressed a total seven non-synonymous SNP (single nucleotide polymorphism) variants Flp-In-293 cells by using Flp (flippase) recombinase system. Of these, F208S S441N were found to be at markedly low levels, whereas their mRNA levels equal those other WT (wild-type). Interestingly, protein expression...

Object. The authors' goal in this study was to understand the frequency and pattern of dural ossification (DO, evaluate effectiveness neuroimaging modalities used identify disease association with posterior longitudinal ligament (OPLL). Methods. One hundred eleven patients OPLL underwent anterior procedures. Of these patients, 17 (15.3%) had associated dura mater. There were 10 cases DO 94 segmental seven nonsegmental (seven continuous mixed-type OPLL). Retrospective evaluation performed by...

✓Extradural unroofing of the optic canal and subsequent mobilization nerve is a useful technique in surgical treatment parasellar tumors; however, drilling procedure itself associated with risk damage. A safer would certainly be beneficial. The ultrasonic bone curette device developed Japan for removal. Its use intradural anterior clinoidectomy opening internal auditory meatus has been reported before. In this article authors describe their experience using extradural patients tumors....

Human ATP-binding cassette transporter ABCB11 (SPGP/BSEP) mediates the elimination of bile salts from liver cells and thereby plays a critical role in generation flow. In present study, we have developed vitro high-speed screening quantitative structure-activity relationship (QSAR) analysis methods to investigate interaction with variety drugs. Plasma membrane vesicles prepared insect overexpressing human were used measure ATP-dependent transport [14C]taurocholate. Over 40 different drugs...

Computed tomography (CT) has been used in the evaluation of 36 spinal neoplasms. Eleven 19 intraspinal and all 17 paraspinal tumors showed positive CT images. is diagnostic value particularly detecting calcified meningiomas. lipomas, neurofi-bromas with bony changes. The size degree bone destruction can be precisely demonstrated by CT.

Accumulating evidence indicates that ATP-binding cassette (ABC) transporter ABCG2 plays a key role in regulating the cellular accumulation of porphyrin derivatives cancer cells and thereby affects efficacy photodynamic therapy diagnosis. The activity efflux can be affected by genetic polymorphisms gene. On other hand, Nrf2, an NF-E2-related transcription factor, has been shown to involved oxidative stress-mediated induction Since patients have demonstrated individual differences their...

In photodynamic diagnosis, 5-aminolevulinic acid (5-ALA) is widely used for the fluorescence-guided resection of malignant brain tumors, where 5-ALA converted to protoporphyrin IX, which exhibits strong fluorescence. Little known, however, about detailed molecular mechanisms underlying 5-ALA–induced To resolve this issue, we analyzed transcriptome profiles genes encoding enzymes, transporters, and a transcription factor involved in porphyrin-biosynthesis pathway. By quantitative real-time...