A5085225502- Antibiotic Resistance in Bacteria
- Lymphoma Diagnosis and Treatment
- HIV/AIDS drug development and treatment
- Drug Transport and Resistance Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Acute Myeloid Leukemia Research
- Pharmacological Effects and Toxicity Studies
- Antimicrobial Resistance in Staphylococcus
- Antifungal resistance and susceptibility
- Infectious Diseases and Tuberculosis
- Streptococcal Infections and Treatments
- Chronic Lymphocytic Leukemia Research
- COVID-19 Clinical Research Studies
- Antibiotic Use and Resistance
- Aquaculture disease management and microbiota
- Nutrition and Health in Aging
- SARS-CoV-2 detection and testing
- Neutropenia and Cancer Infections
- Vibrio bacteria research studies
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- CNS Lymphoma Diagnosis and Treatment
- Long-Term Effects of COVID-19
Yokohama Municipal Citizen's Hospital
2019-2025
Fujita Health University
2020-2025
National Center for Global Health and Medicine
2014-2025
Center for Global Health
2025
The University of Texas Health Science Center at Houston
2019-2023
Australian National University
2022
St. Luke's International Hospital
2016-2021
St. Luke's International University
2021
National Institute of Infectious Diseases
2021
Fujita Health University Hospital
2020
Natural History of Asymptomatic SARS-CoV-2 Infection The outbreak on the cruise ship Diamond Princess led to identification 96 persons who were infected with but asymptomatic...
The rapid deployment of mRNA vaccines for SARS-CoV-2, such as BNT162b2 (BioNTech-Pfizer) and mRNA-1273 (Moderna), provided a critical tool in combating the COVID-19 pandemic. While their short-term safety efficacy were demonstrated clinical trials, rare adverse events, including hemorrhagic strokes, have been reported after widespread use. However, long-term biodistribution effects remain underexplored. This study aimed to investigate presence SARS-CoV-2 spike protein brain tissues patients...
Several preclinical and clinical studies suggest the importance of naturally occurring polymorphisms drug transporters in individual difference response. To functionally validate nonsynonymous ABCB1 (P-glycoprotein/MDR1) vitro, we generated SNP variant forms (i.e., S400N, R492C, R669C, I849M, A893P, A893S, A893T, M986V, A999T, P1051A, G1063A) expressed them Sf9 cells. The kinetic properties (Km Vmax) those variants were analyzed by measuring ATPase activity to obtain profile for each toward...
One single-nucleotide polymorphism (SNP), 538G>A (Gly180Arg), in the ABCC11 gene determines type of earwax. The G/G and G/A genotypes correspond to wet earwax, whereas A/A corresponds dry type. Wide ethnic differences exist frequencies those alleles, reflecting global migratory waves ancestors humankind. We herein provide evidence that this genetic has an effect on N-linked glycosylation ABCC11, intracellular sorting, proteasomal degradation variant protein. Immunohistochemical studies with...
We evaluated the performance of Verigene Gram-Negative Blood Culture Nucleic Acid Test (BC-GN; Nanosphere, Northbrook, IL, USA), an automated multiplex assay for rapid identification positive blood cultures caused by 9 Gram-negative bacteria (GNB) and detection genes associated with β-lactam resistance. The BC-GN can be performed directly from 5 minutes hands-on 2 hours run time per sample. A total 397 GNB were analyzed using assay. Of samples, 295 simulated samples prepared inoculating into...
Human ATP-binding cassette transporter ABCB11 (SPGP/BSEP) mediates the elimination of bile salts from liver cells and thereby plays a critical role in generation flow. In present study, we have developed vitro high-speed screening quantitative structure-activity relationship (QSAR) analysis methods to investigate interaction with variety drugs. Plasma membrane vesicles prepared insect overexpressing human were used measure ATP-dependent transport [14C]taurocholate. Over 40 different drugs...
A growing number of studies suggest the potential role asymptomatic SARS-CoV-2 carriers as a major driver COVID-19 pandemic; however, virological assessment infection has largely been limited to reverse transcription-PCR (RT-PCR), which can be persistently positive without necessarily indicating presence viable virus (e.g., replication-competent virus). Here, we evaluated infectivity by detecting SARS-CoV-2-induced cytopathic effects on Vero cells using longitudinally obtained nasopharyngeal...
Abstract AmpC-mediated cephalosporin resistance occurs in 1.0% to 3.3% of Escherichia coli isolates due production either plasmid-mediated AmpC (p-AmpC) or chromosomal (c-AmpC). Data on the prevalence and molecular characteristics AmpC-producing E. pediatric patients are limited. We analyzed clinical strains with phenotype consistent isolated from at a hospital Japan between 2015 2022. Sequence types, genes, relevant mutations were identified through whole genome sequencing. Promoter...
Carbapenem-resistant Gram-negative bacilli (CRGNB), especially Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii, are critical pathogens associated with excess morbidity mortality. To elucidate their molecular epidemiology clinical outcomes in Japan, patients CRGNB were enrolled the MDR organisms research network (MDRnet) consisting of eight tertiary care facilities. Between 2019 2022, 246 unique carbapenem-resistant Enterobacterales (CRE), P. (CRPA) A. baumannii (CRAB)...
ABSTRACT The genus Aeromonas is increasingly implicated in human infections. However, accurate species-level identification remains challenging, particularly clinical microbiology laboratories. This study aimed to develop a multiplex polymerase chain reaction (PCR) assay identify four species— hydrophila , caviae veronii and dhakensis— most frequently associated with infectious diseases. A total of 788 whole genome sequencing (WGS) data sets from 31 species were analyzed open reading frames...
The increased identification of carbapenem-resistant Pseudomonas aeruginosa (CR-PA) is an ongoing concern. However, information on the evolving antimicrobial resistance profile and molecular epidemiology CR-PA over time scarce. Thus, we conducted a cross-sectional analysis to investigate phenotypic genotypic characteristics recovered different periods, focusing isolates exhibiting ceftolozane/tazobactam phenotype.A total 169 isolated from clinical specimens at single centre in Houston, TX,...
ABSTRACT Carbapenemase-producing Enterobacterales (CPEs) are one of the top priority antimicrobial-resistant pathogens. Among CPEs, those producing acquired metallo-β-lactamases (MBLs) considered particularly problematic as few agents active against them. Imipenemase (IMP) is most frequently encountered MBL in Japan, but comprehensive assessment clinical and microbiological features IMP-producing infection remains scarce. Here, we retrospectively evaluated 62 patients who were hospitalized...
The Controlling Nutritional Status (CONUT) score predicts the prognosis in several tumors. However, its prognostic significance multiple myeloma (MM) remains unclear. present study investigated correlation between CONUT and survival outcomes of MM patients. A total 178 patients newly diagnosed with were retrospectively enrolled. Patients a high (≥5) had significantly shorter median overall (OS) than those low (≤4) (33 vs. 57 months, p < .001). In multivariate analysis among International...
Several immunochromatographic serological test kits have been developed to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies, but their relative performance and potential clinical utility is unclear.Three commercially available were evaluated using 99 serum samples collected from 29 patients diagnosed with disease 2019 (COVID-19) 100 healthy volunteers in 2017 as negative controls.The specificity of the IgM IgG antibodies showed comparable results among...
Secondary fungal infections are a critical problem that accompany immunosuppressive therapy for severe coronavirus disease 2019 (COVID-19). We report fatal case of COVID-19 with disseminated mucormycosis diagnosed during autopsy. A 58-year-old man diabetes was hospitalized and treated remdesivir, systemic steroids tocilizumab. Following treatment, he provided extracorporeal membrane oxygenation support. However, died multiple organ failure accompanied by pulmonary kidney infarction, as...