The aims of this study were to isolate LAB with anti-Listeria activity from salami samples, characterize the bacteriocin/s produced by selected isolates, semi-purify them and evaluate their effectiveness for control Listeria monocytogenes during manufacturing in a pilot scale. Two isolates (differentiated RAPD-PCR) presented against 22 out 23 L. strains bacteriocin MBSa2, while MBSa3 inhibited all addition several other Gram-positive bacteria both antimicrobials identified as Lactobacillus...

Milk is an excellent source of well balanced nutrients and also exhibits a range biological activities that influence digestion, metabolic responses to absorbed nutrients, growth development specific organs, resistance disease. Goat milk as close perfect food possible in nature.Bioactive peptides have been defined protein fragments positive impact on body functions or conditions may ultimately health. It can be generated during fermentation by the proteolytic activity starter cultures. The...

The present work discusses the technological and new selection criteria that should be included for selecting lactic acid bacteria production of fermented meat. Lactic isolated from Bulgarian traditional "lulanka" salami was studied regarding some positive parameters (growth at different temperature, pH, proteolytic activity). presence genes related to virulence factors, biogenic amines, vancomycin resistance were presented in low frequency bacteria. On other hand, antimicrobial peptides...

The objective of this study was to isolate multifunctional bacteriocin-producing strains; characterize the expressed bacteriocin for control Listeria monocytogenes and vancomycin-resistant Enterococcus; evaluate safety studied explore their antifungal activity.Two Pediococcus strains were isolated from silage samples obtained an organic farm in Belogradchik, Bulgaria. identified by 16S rRNA sequencing analysis characterized as bacteriocins producers. Strong antimicrobial activity detected...

The aim of this study was to explore the biochemical and genetic features two-peptide bacteriocin produced by a Lactobacillus plantarum strain isolated from Italian type salami in Brazil (Lb. MBSa4). Identification bacteriocinogenic Lb. MBSa4 performed 16S rRNA sequencing. Expressed evaluated for spectrum activity, heat pH stability, mechanism action, molecular mass. Partial purification achieved cation-exchange, reversed phase - HPLC. Total DNA extracted tested presence previously described...

Aims: The aim of this work was to isolate lactic acid bacteria (LAB) strains from Mongolian tarag (a traditionally homemade yoghurt) displaying antimicrobial activities against food‐borne pathogens, identify inhibitory substances and study the kinetics their production. Methods Results: Inhibitory substance‐producing bacterial were isolated tarag. From 300 clones, 31 able inhibit growth indicator strain Lactobacillus bulgaricus 340. One most active identified as delbrueckii subsp. lactis T31...

We examined 62 strains and 21 trade starter cultures from the collection of LB Bulgaricum PLC for proteolytic antimicrobial activity lactic acid bacteria (LAB) grown in goat milk. The aim this study was to investigate fermentation caseins, α-lactalbumin β-lactoglobulin by LAB, using o-phthaldialdehyde (OPA) spectrophotometric assay sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). proteolysis targeted mainly especially β-casein. Whey proteins were proteolyzed,...

The study aimed at determining the biochemical characteristics of bacteriocin produced by Lactobacillus sakei MBSa1, isolated from salami, correlating results with genetic features producer strain.Identification strain MBSa1 was performed 16S rDNA sequencing. tested for spectrum activity, heat and pH stability, mechanism action, molecular mass amino acid sequence when purified cation-exchange reversed-phase HPLC. Genomic DNA genes commonly present in Lact. sakei. Bacteriocin heat-stable,...

The exploration of habitats with unusual environment and poorly explored areas such as Antarctica is one the strategies for discovery new biologically active substances and/or producers. aim this study was to identify actinomycetes isolated from soils island Livingston - investigate their potential synthesize antibacterial agents against phytopathogens. Twenty-three actinomycete strains were object study. Using PCR (polymerase chain reaction) amplification all affiliated genus Streptomyces....

Twenty morphologically different strains were chosen from French wheat sourdough isolates. Cells Gram-positive, non-spore-forming, non-motile rods. The isolates identified using amplified-fragment length polymorphism, randomly amplified polymorphic DNA and 16S rRNA gene sequence analysis. All members of the genus Lactobacillus . They as representing plantarum , paralimentarius sanfranciscensis spicheri sakei However, two (LP38 T LP39) could be clearly discriminated recognized species on...

Three lactic acid bacteria (LAB) strains identified as Lactobacillus plantarum, Lactobacillusbrevis, and Lactobacillussakei isolated from meat products were tested for their ability to utilize grow on xylooligosaccharides (XOSs). The extent of carbohydrate utilization by the studied was analyzed HPLC. All three showed preferences degree polymerization (DP). added oligosaccharides induced LAB form end-products typical mixed-acid fermentation. XOSs microorganisms requires action important...

Bees are one of the best-known and, at same time, perhaps most enigmatic insects on our planet, known for their organization and social structure, being essential pollination agricultural crops several other plants, playing an role in food production balance ecosystems, associated with high-value-added inputs, a unique universe relation to bees’ microbiota. In this review, we summarize information regarding different varieties bees, emphasis specificity related microbial variations....