A5024090135- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Probiotics and Fermented Foods
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Protein Hydrolysis and Bioactive Peptides
- Meat and Animal Product Quality
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Plant Parasitism and Resistance
- Microbial Metabolites in Food Biotechnology
- Banana Cultivation and Research
- Microbial Metabolism and Applications
- Gut microbiota and health
- Polar Research and Ecology
- Microbial Metabolic Engineering and Bioproduction
- Bacteriophages and microbial interactions
- Fungal Biology and Applications
- Bacterial biofilms and quorum sensing
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Nematode management and characterization studies
- Phytochemicals and Antioxidant Activities
- Infant Nutrition and Health
Sofia University "St. Kliment Ohridski"
2014-2024
ORCID
2022
The present work discusses the technological and new selection criteria that should be included for selecting lactic acid bacteria production of fermented meat. Lactic isolated from Bulgarian traditional "lulanka" salami was studied regarding some positive parameters (growth at different temperature, pH, proteolytic activity). presence genes related to virulence factors, biogenic amines, vancomycin resistance were presented in low frequency bacteria. On other hand, antimicrobial peptides...
We examined 62 strains and 21 trade starter cultures from the collection of LB Bulgaricum PLC for proteolytic antimicrobial activity lactic acid bacteria (LAB) grown in goat milk. The aim this study was to investigate fermentation caseins, α-lactalbumin β-lactoglobulin by LAB, using o-phthaldialdehyde (OPA) spectrophotometric assay sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). proteolysis targeted mainly especially β-casein. Whey proteins were proteolyzed,...
The exploration of habitats with unusual environment and poorly explored areas such as Antarctica is one the strategies for discovery new biologically active substances and/or producers. aim this study was to identify actinomycetes isolated from soils island Livingston - investigate their potential synthesize antibacterial agents against phytopathogens. Twenty-three actinomycete strains were object study. Using PCR (polymerase chain reaction) amplification all affiliated genus Streptomyces....
ABSTRACTThe members of genus Burkholderia are various microorganisms, which occupy surprisingly wide range ecological niches—soil, water (including marine water), rhizosphere, animals and human. The includes over 30 species that important causes human, animal plant diseases, as well organisms useful in promoting growth bioremediation. Extremely is the question if "good" "bad" strains equivalent their nature.Keywords: Burkholderiabiodiversitybioagentpathogens
This study aims at the identification and characterization of five actinobacterial strains with presumed belonging to species
During the last few decades, main focus of numerous studies has been on human breast milk microbiota and its influence infant intestinal overall health. The presence lactic acid bacteria in affects both quantitative qualitative composition gut microbiota. aim this study was to assess most frequently detected cultivable rod-shaped lactobacilli, specific for healthy Bulgarian women fecal samples their infants over first month life, 14 mother-infant tandem pairs. Additionally, we evaluated...
During the last 20 years, causative agents of bacterial spot tomato and pepper have been subjected to many studies reclassifications. According current data, species are four (X. euvesicatoria, X. vesicatoria, gardneri, perforans) cause similar symptoms in plants but possess different phenotypic properties. This work provides full metabolic characteristics obtained by Biolog system spot's xanthomonads based on a large selection strains from vegetable-producing regions Bulgaria with accent...
The intestinal lactic acid microflora of the edible snail Cornu aspersum was studied by culture-based methods and phenotypically molecularly characterized. antibacterial activity bacteria (LAB) isolates investigated. Snails in different stages development were collected from farms located several regions Bulgaria. One hundred twenty-two isolates, belonging to group LAB, characterized morphologically divided into four groups. Representative each morphological type subjected phenotypic...
A novel antibacterial substance produced by a strain isolated from Bulgarian yellow cheese was characterized. The producer identified molecular typing to belong the species Lactobacillus delbrueckii, which is rare of bacteriocins. inhibitory agent heat stable and active against lactic acid bacteria several food-borne pathogens : Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Yersinia enterocolitica Y. pseudotuberculosis. Its sensitivity amylolitic...
The antimicrobial activity of 18 different extracts from in vivo and vitro grown L. album plants was evaluated against clinical bacteria yeasts using the well diffusion method. All used demonstrated antibacterial activity, whereas only water leaves (in vivo) possessed antifungal Candida albicans NBIMCC 72 glabrata 8673 (14 20 mm diameter inhibition zones MIC 10 mg/ml, respectively). methanol ethanol obtained propagated had a broader spectrum than those plants, while opposite tendency...
Bacteriophages have greatly engaged the attention of scientists worldwide due to continuously increasing resistance phytopathogenic bacteria commercially used chemical pesticides. However, knowledge regarding phages is still very insufficient and must be expanded. This paper presents results isolation, characterization, evaluation potential 11 phage isolates as natural predators a severe bacterium—Xanthomonas euvesicatoria. Phages were isolated from rhizosphere tomato plants with symptoms...
Seventy-one Xanthomonas strains isolated from infected pepper and tomato plants with bacterial spot Bulgaria Macedonia were examined in this study. The pathotypes races of the determined. pepper-tomato pathotype predominated among Bulgarian strains, whereas Macedonian belonged to pathotype. On basis their phenotypic characteristics causal agents on possessed properties at least three species (X. euvesicatoria, X. vesicatoria/X. perforans gardneri). identification thirty euvesicatoria was...
Nine strains of Erwinia amylovora were isolated from new host plants in Bulgaria D chokeberry and strawberry. The characterized morphologically biochemically using the API 20E BIOLOG system. It was established that they showed three different metabolic profiles, not found by previous studies E. amylovora. All identified as due to their fingerprint patterns obtained identification confirmed PCR amplification a specific region plasmid pEA29 genome ams-region. This study is first...
The proteolytic activity of 276 natural isolates from kefir grains was investigated. Evidence for extracellular demonstrated 49 Lactobacillus strains. One strain, DR22x, selected as a producer proteinases. DR22x strain produces cell-wall-bound proteinase. proteinase removed the cell envelope by washing cells with Ca2+- free buffer. crude extract showed highest at pH 7.2 and 37 °C. shown to be maximal in late exponential growth. A study using several protease inhibitors suggested that this is...
ABSTRACT We report the draft genome sequences of two Xanthomonas euvesicatoria strains from Balkan Peninsula, which were isolated symptomatic pepper plants. The availability these will facilitate development modern genotyping assays for X. and to define targets resistance breeding.
Bacterial spot of pepper and tomato is caused by at least three species Xanthomonas, among them two pathovars Xanthomonas euvesicatoria, which are responsible for significant yield losses on all continents. In order to trace back the spread bacterial pathogens within countries, we developed first multilocus variable number tandem repeat analyses (MLVA) scheme pepper- tomato-pathogenic strains X. euvesicatoria. this work, assessed numbers DNA sequencing 16 loci applied new tool analyse a...
Sub-species diversity of pepper populations Xanthomonas euvesicatoria in Bulgaria and Macedonia 2012 was the object this study. Species determination 44 strains performed by molecular methods using two pairs species-specific primers RFLP (restriction fragment length polymorphism) analysis 16S-23S ITS region with HpaII. The were characterized genotypic phenotypic properties. evaluated RAPD (random amplified polymorphic DNA) technique. Primer CUGEA-6 differentiated groups, one which included...
During the last few decades, main focus of numerus studies has been on human breast milk microbiota and its influence infant intestinal overall health. The presence lactic acid bacteria in affects both quantitative qualitative composition gut microbiota. aim this study was to assess dominant cultivable rod-shaped bacteria, specific for healthy Bulgarian women fecal samples their infants over first month life, 14 mother-infant tandem pairs. Additionally, we evaluated strain diversity among...
Bacterial spot is an important disease of pepper in Bulgaria and Macedonia. For characterization Xanthomonas species associated with bacterial spot, 161 strains were collected from various field pepper-growing regions. Among them, 131 identified as euvesicatoria 30 vesicatoria using species-specific primers polymerase chain reaction followed by restriction fragment length polymorphism analysis. To assess the genetic diversity strains, two methods (Random Amplified Polymorphic DNA Repetitive...