A5082637431- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Insect Resistance and Genetics
- Transgenic Plants and Applications
- Plant Disease Management Techniques
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Virus-based gene therapy research
- Plant and Fungal Interactions Research
- Chromosomal and Genetic Variations
- Agricultural pest management studies
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Fungal Diseases
- Plant Pathogens and Resistance
- Viral Infections and Immunology Research
- Genomics and Phylogenetic Studies
- Plant Genetic and Mutation Studies
- Insect and Pesticide Research
- Berry genetics and cultivation research
- Phytochemistry and biological activities of Ficus species
- Insect symbiosis and bacterial influences
- Plant pathogens and resistance mechanisms
Institute of Soil Science “Nikola Poushkarov”
2012-2022
Agricultural Academy
2013-2022
Plant Protection Institute
2007-2022
Agrobioinstitute
2005-2007
The exploration of habitats with unusual environment and poorly explored areas such as Antarctica is one the strategies for discovery new biologically active substances and/or producers. aim this study was to identify actinomycetes isolated from soils island Livingston - investigate their potential synthesize antibacterial agents against phytopathogens. Twenty-three actinomycete strains were object study. Using PCR (polymerase chain reaction) amplification all affiliated genus Streptomyces....
ABSTRACTThe members of genus Burkholderia are various microorganisms, which occupy surprisingly wide range ecological niches—soil, water (including marine water), rhizosphere, animals and human. The includes over 30 species that important causes human, animal plant diseases, as well organisms useful in promoting growth bioremediation. Extremely is the question if "good" "bad" strains equivalent their nature.Keywords: Burkholderiabiodiversitybioagentpathogens
The aim of the present study is to analyze dynamics microbiological changes white strawberry fruits (Fragaria ananassa ‘Snow White’) grown in a greenhouse under five irrigation and fertil¬ization regimes, stored refrigerated state determine their shelf life depending on influence cultivation time fruiting storage, determined by standard methods for analysis epiphytic microflora. It was found that compared initial state, period, increase fruit losses, total number microorganisms, molds,...
During the last 20 years, causative agents of bacterial spot tomato and pepper have been subjected to many studies reclassifications. According current data, species are four (X. euvesicatoria, X. vesicatoria, gardneri, perforans) cause similar symptoms in plants but possess different phenotypic properties. This work provides full metabolic characteristics obtained by Biolog system spot's xanthomonads based on a large selection strains from vegetable-producing regions Bulgaria with accent...
Potato viruses cause enormous economic loss in agriculture production. Potatoes can be infected by a number of different that affect negatively the harvest and tuber quality. Direct effective drugs against plant virus diseases are still not available control is only applied as agricultural measures pesticides vectors. Y (PVY) transmitted aphids non-persistent manner on account using insecticides to prevent spread infection useless. Breeding resistant cultivars proved always solution problem...
ABSTRACTIntroduction of human lactoferrin (hLF) gene, a cationic iron-binding glycoprotein, in the genome commercial Bulgarian barley (Hordeum vurgare L.) cultivar was performed via particle bombardment. A rapid, efficient, and reproducible system used to generate green, fertile, transgenic plants. Mature zygotic embryos from dry seeds malting Ruen were bombarded with two plasmids (pAFT105 pUBLF) containing gene. The co-transformed either pDM302 selectable marker gene (bar) for herbicide...
Seventy-one Xanthomonas strains isolated from infected pepper and tomato plants with bacterial spot Bulgaria Macedonia were examined in this study. The pathotypes races of the determined. pepper-tomato pathotype predominated among Bulgarian strains, whereas Macedonian belonged to pathotype. On basis their phenotypic characteristics causal agents on possessed properties at least three species (X. euvesicatoria, X. vesicatoria/X. perforans gardneri). identification thirty euvesicatoria was...
Sub-species diversity of pepper populations Xanthomonas euvesicatoria in Bulgaria and Macedonia 2012 was the object this study. Species determination 44 strains performed by molecular methods using two pairs species-specific primers RFLP (restriction fragment length polymorphism) analysis 16S-23S ITS region with HpaII. The were characterized genotypic phenotypic properties. evaluated RAPD (random amplified polymorphic DNA) technique. Primer CUGEA-6 differentiated groups, one which included...
Bacterial spot is an important disease of pepper in Bulgaria and Macedonia. For characterization Xanthomonas species associated with bacterial spot, 161 strains were collected from various field pepper-growing regions. Among them, 131 identified as euvesicatoria 30 vesicatoria using species-specific primers polymerase chain reaction followed by restriction fragment length polymorphism analysis. To assess the genetic diversity strains, two methods (Random Amplified Polymorphic DNA Repetitive...
Summer snowflake/Leucojum aestivum L./is a medical bulbous plant which belongs to family Amarillydaceae. It is wild-spread as natural populations in Bulgaria and valuable source of galantamine. A few virus fungal diseases have been described on plants Amaryllidaceae but only pathogens summer snowflake. This paper the first report Serratia plymuthica Stenotrophomonas maltophilia causal agents bulb decay To our knowledge, this also second phytopathogenic isolates identified two species.The...
The identification of 22 isolates as Burkholderia cepacia and B. gladioli was confirmed by PCR amplification with species- specific primers CMG 16–1, G 16–2, 23–1, CM 23–2. strains were characterized phenotypically BIOLOG metabolic profile, antibiotic susceptibility, interactions other microorganisms. A greater diversity among than revealed. phytopathogenic the clinic clearly distinguished in studied properties. differed from their abilities, significantly lower susceptibility to tested...
Potato virus Y (PVY) is the reason for significant losses in potato crop yearly. The recent technologies molecular biology have given insights on possibilities control of viral infections at epigenetic level. In present study, we obtained a DNA template complementary to key sequence NIb genetic region PVY. leaves were inoculated with dsRNAs and siRNAs 48 h before inoculation PVY strain N/NTN. treated plants did not develop any signs disease. Double antibody sandwich еnzyme-linked...
Today the world population is increasing at most rapid rate ever. It fore cast that by year 2050, world's will double to nearly 12 billion people. In fact it has been esti...